- Clone
- HMα2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- α2 integrin, VLA-2 α chain, Integrin α2 chain, ITGA2, HMa2
- Isotype
- Armenian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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103525 | 100 µg | 231 CHF | ||||
103526 | 1 mg | 710 CHF |
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD49b is a 150 kD glycoprotein, also known as α2 integrin, VLA-2 α chain, Integrin α2 chain, and HMα2. It is a member of the integrin family, expressed on NK cells, a subset of splenic CD4+ T cells, NK-T cells, intestinal intraepithelial and lamina propria lymphocytes, epithelial cells, and platelets. By associating with CD29 (integrin β1 subunit), CD49b forms the VLA-2 (integrin α2β1 ) complex. It plays a critical role in both adhesion and lymphocyte activation. The primary ligands for CD49b are collagen, laminin, and fibronectin. The HMα2 antibody has been shown to be useful for partially blocking CD49b mediated interactions with collagen. Additionally, this antibody blocks staining of splenic NK cells by the monoclonal antibody DX5.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Mouse colon carcinoma cell line Colon26
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
IHC-F, IP, Block - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence on frozen sections4, and blocking of cell adhesion1-3. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 103525 & 103526).
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Application References
(PubMed link indicates BioLegend citation) -
- Miyake S, et al. 1994. Eur. J. Immunol. 24:2000. (Block, IP)
- Noto K, et al. 1995. Int. Immunol. 7:835. (Block)
- Arase H, et al. 2001. J. Immunol. 167:1141. (Block)
- Zhang Z, et al. 2008. Blood 111:1980. (IF)
- Product Citations
-
- RRID
-
AB_2888793 (BioLegend Cat. No. 103525)
AB_2888793 (BioLegend Cat. No. 103526)
Antigen Details
- Structure
- Integrin family, associates with CD29 (β1 integrin), 150 kD
- Distribution
-
NK cells, subset splenic CD4+ T cells, NK-T cells, intestinal intraepithelial and lamina propria lymphocytes, epithelial cells, platelets
- Function
- Adhesion, lymphocyte activation
- Ligand/Receptor
- Collagen, laminin, fibronectin
- Cell Type
- Epithelial cells, Lymphocytes, NK cells, NKT cells, Platelets, T cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Sasaki K, et al. 2003. Int. Immunol. 15:701.
3. Inoue O, et al. 2003. J. Cell Biol. 160:769. - Gene ID
- 16398 View all products for this Gene ID
- UniProt
- View information about CD49b on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD49b Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
FITC anti-mouse CD49b | HMα2 | FC |
PE anti-mouse CD49b | HMα2 | FC |
Purified anti-mouse CD49b | HMα2 | FC,IHC-F,IP,Block |
Alexa Fluor® 488 anti-mouse CD49b | HMα2 | FC |
Alexa Fluor® 647 anti-mouse CD49b | HMα2 | FC |
Purified anti-mouse CD49b (Maxpar® Ready) | HMα2 | FC,CyTOF® |
APC anti-mouse CD49b | HMα2 | FC |
PE/Cyanine7 anti-mouse CD49b | HMα2 | FC |
PerCP/Cyanine5.5 anti-mouse CD49b | HMα2 | FC |
Biotin anti-mouse CD49b | HMα2 | FC |
TotalSeq™-A0421 anti-mouse CD49b | HMα2 | PG |
TotalSeq™-C0421 anti-mouse CD49b | HMα2 | PG |
Ultra-LEAF™ Purified anti-mouse CD49b | HMα2 | FC,IHC-F,IP,Block |
TotalSeq™-B0421 anti-mouse CD49b | HMα2 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-mouse CD49b
C57BL/6 splenocytes stained with HMα2 PE and 145-2C11 FITC -
PE anti-mouse CD49b
C57BL/6 splenocytes stained with HMα2 PE and 145-2C11 FITC -
Purified anti-mouse CD49b
C57BL/6 mouse splenocytes stained with purified HMα2, follow... -
Alexa Fluor® 488 anti-mouse CD49b
C57BL/6 mouse splenocytes stained with HMα2 Alexa Fluor® 488... -
Alexa Fluor® 647 anti-mouse CD49b
C57BL/6 mouse splenocytes stained with HMα2 Alexa Fluor® 647... -
Purified anti-mouse CD49b (Maxpar® Ready)
C57BL/6 mouse splenocytes stained with 152Sm-anti-CD3e (145-... -
APC anti-mouse CD49b
C57BL/6 splenocytes were stained with NK1.1 PE and CD49b (cl... -
PE/Cyanine7 anti-mouse CD49b
C57BL/6 splenocytes were stained with NK1.1 APC and CD49b (c... -
PerCP/Cyanine5.5 anti-mouse CD49b
C57BL/6 splenocytes were stained with NK-1.1 APC and CD49b (... -
Biotin anti-mouse CD49b
C57BL/6 splenocytes were stained with NK1.1 APC and biotinyl... -
TotalSeq™-A0421 anti-mouse CD49b
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TotalSeq™-C0421 anti-mouse CD49b
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Ultra-LEAF™ Purified anti-mouse CD49b
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TotalSeq™-B0421 anti-mouse CD49b
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