Alexa Fluor® 488 anti-mouse Ly-6C Antibody

Product Details

Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

L3 cloned CTL cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Blue Laser (488 nm)

Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C5⁸.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells^1-3 and immunohistochemistry of frozen sections⁴.

Application References

(PubMed link indicates BioLegend citation)

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed

Product Citations

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RRID

AB_10640820 (BioLegend Cat. No. 128021)
AB_10639728 (BioLegend Cat. No. 128022)

Antigen Details

Structure

14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.

Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8⁺ T cells.

Cell Type

Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells

Biology Area

Immunology

Molecular Family

CD Molecules

Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID

17067 View all products for this Gene ID

UniProt

View information about Ly-6C on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Other Formats

Concentration & Expiration Lookup

Certificate of Analysis