- Clone
- RMG1-1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Immunoglobulin G1
- Isotype
- Rat IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A_RMG1-1_AF647_IgG1_Antibody_FC_1_041714.jpg A_RMG1-1_AF647_IgG1_Antibody_FC_1_041714.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/RMG1-1_AF647_IgG1_Antibody_FC_1_041714.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with purified anti-human CD3 (clone UCHT1, mouse IgG1 isotype, filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG1 (clone RMG1-1) Alexa Fluor® 647. -
FVB/N mouse frozen thymus section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of purified anti-rat CD90/mouse CD90.1 (clone OX-7) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 647 Goat anti-mouse IgG1 (clone RMG1-1) (red) for two hours at room temperature. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective. -
IHC staining of Alexa Fluor® 647 anti-mouse IgG1 on formalin-fixed paraffin-embedded human tonsil. Following antigen retrieval using Tris-EDTA, pH 9.0, the tissue was incubated with 5 µg/mL of purified mouse anti-CD8a (Cat. No. 372902) at 4°C. This was followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG1 (clone RMG1-1) at 2 µg/mL. Nuclei were counterstained with DAPI (blue, panel A) (Cat. No. 422801) and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale: 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
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406617 | 25 µg | 89 CHF | ||||
406618 | 100 µg | 194 CHF |
The RMG1-1 monoclonal antibody reacts with immunoglobulin G1 (IgG1) in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG1-1 monoclonal antibody may be used as a primary or secondary reagent for ELISA or immunofluorescent analysis.
Product DetailsProduct Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse Ig cocktail
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
IHC-F, IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 µg/mL is suggested. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Red Laser (633 nm)
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Application References
(PubMed link indicates BioLegend citation) - Product Citations
- RRID
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AB_2563476 (BioLegend Cat. No. 406617)
AB_2563477 (BioLegend Cat. No. 406618)
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