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Alexa Fluor® 647 Goat anti-mouse IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4053 (See other available formats)
Regulatory Status
RUO
Isotype
Goat Polyclonal IgG
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Product Citations
publications
Poly4053_A647_IgG_Antibody_1_111120
Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of purified anti-human CD309/VEGFR2 (clone A16085I) and Alexa Fluor® 594 anti-human PNAD (clone MECA-79) (green) at 4°C overnight, followed by 2.5 µg/ml of Alexa Fluor® 647 anti-mouse IgG (clone Poly4053) (red) for two hours at room temperature. The image was captured with a 10X objective.
  • Poly4053_A647_IgG_Antibody_1_111120
    Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of purified anti-human CD309/VEGFR2 (clone A16085I) and Alexa Fluor® 594 anti-human PNAD (clone MECA-79) (green) at 4°C overnight, followed by 2.5 µg/ml of Alexa Fluor® 647 anti-mouse IgG (clone Poly4053) (red) for two hours at room temperature. The image was captured with a 10X objective.
See Alexa Fluor® 647 spectral data
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405322 100 µg 183 CHF
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Description

This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Goat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 2.5 - 5.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.

Application References

(PubMed link indicates BioLegend citation)
  1. Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed
  2. Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC)
  3. Winters T, et al. 2014. EMBO J. 33:1256-70.
Product Citations
  1. Smith R, et al. 2017. Biophys J. 10.1016/j.bpj.2017.05.005. PubMed
  2. Jung WH, et al. 2019. Cells. 8:813. PubMed
  3. Zhao Y, et al. 2020. Immunity. 51(6):1059-1073.e9.. PubMed
  4. özdilek A, et al. 2020. Proc Natl Acad Sci U S A. 117:1280. PubMed
  5. Pandey N, et al. 2020. J Cell Biol. 219:00:00. PubMed
  6. Zheng W, et al. 2020. Nature. 577:543. PubMed
  7. Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed
  8. Bose S, et al. 2020. Nat Biomed Eng. 4:814. PubMed
  9. Scott WA, et al. 2021. PLoS Genet. 17:e1009909. PubMed
  10. Middleton DR, et al. 2021. J Bacteriol. 203:. PubMed
  11. Khan AA, et al. 2022. Bioconjug Chem. . PubMed
  12. Wong JJ, et al. 2021. Sci Adv. 7:. PubMed
  13. Chen BR, et al. 2021. Elife. 10:. PubMed
  14. Della Marina A, et al. 2021. Cells. 10:. PubMed
  15. Pruett L, et al. 2020. Laryngoscope. 130:2432. PubMed
  16. Chen BR, et al. 2022. Bio Protoc. 12:e4413. PubMed
RRID
AB_2563045 (BioLegend Cat. No. 405322)
Go To Top Version: 4    Revision Date: 11.11.2020

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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