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Alexa Fluor® 700 anti-human CD8a Antibody

Pricing & Availability
Clone
RPA-T8 (See other available formats)
Regulatory Status
RUO
Workshop
IV T171
Other Names
T8, Leu2
Isotype
Mouse IgG1, κ
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Product Citations
publications
RPA-T8_Alx700_072408.jpg
Human peripheral blood lymphocytes stained with RPA-T8 Alexa Fluor® 700
  • RPA-T8_Alx700_072408.jpg
    Human peripheral blood lymphocytes stained with RPA-T8 Alexa Fluor® 700
See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability Save
301027 25 µg 126 CHF
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301028 100 µg 268 CHF
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Description

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation, and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

The RPA-T8 antibody does not block the binding of HIT8a antibody to CD8a. Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed frozen sections3 and costimulation of T cell responses4. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 301073 & 301074).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  3. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  4. Magidovich E, et al. 2007. P. Natl. Acad. Sci. USA 104:13022.
  5. Thakarl D, et al. 2008.J. immunol. 180:7431. PubMed
  6. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  7. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  10. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
Product Citations
  1. Ickrath P, et al. 2019. Biomed Rep. 10:119. PubMed
  2. Ickrath P, et al. 2018. Int J Mol Med. 42:1116. PubMed
  3. Swadling L, et al. 2020. Cell Rep. 30:687. PubMed
  4. Freeman ZT, et al. 2020. J Clin Invest. 130:1405. PubMed
  5. Katsuyama E, et al. 2020. Cell Reports. 30(1):112-123.e4.. PubMed
  6. Boland J, et al. 2014. BMJ Support Palliat Care. 4:357. PubMed
  7. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  8. Iwabuchi R, et al. 2021. Frontiers in Immunology. 12:643040. PubMed
  9. Nielsen CM, et al. 2021. Cell Reports Medicine. 2(3):100207. PubMed
  10. Carre C, et al. 2021. iScience. 24:102970. PubMed
  11. Pagel J, et al. 2020. Front Immunol. 11:565257. PubMed
  12. Gatla H, et al. 2022. Front Med Technol. 4:850565. PubMed
  13. Lee-Chang C, et al. 2021. J Exp Med. 218:. PubMed
  14. Vanshylla K, et al. 2022. Cell Host Microbe. 30:69. PubMed
  15. Vadrevu KM, et al. 2022. Sci Rep. 12:12038. PubMed
  16. Oostindie SC, et al. 2022. Nat Biotechnol. :. PubMed
  17. Alhaj Hussen K, et al. 2020. Front Immunol. 11:579776. PubMed
  18. Chandran A, et al. 2022. Cell Rep Med. 3:100557. PubMed
  19. Lorvik KB, et al. 2021. Front Immunol. 12:744155. PubMed
  20. Terahara K, et al. 2022. iScience. 25:104959. PubMed
RRID
AB_493744 (BioLegend Cat. No. 301027)
AB_493745 (BioLegend Cat. No. 301028)

Antigen Details

Structure
Ig superfamily, homodimer or heterodimer with CD8β, 32-34 kD
Distribution

Majority of thymocytes, T cell subset, NK cells

Function
MHC class I co-receptor, thymic differentiation, T cell activation
Ligand/Receptor
MHC Class I molecules
Cell Type
Dendritic cells, NK cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.

Gene ID
925 View all products for this Gene ID
UniProt
View information about CD8alpha on UniProt.org
Go To Top Version: 4    Revision Date: 07.13.2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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