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Anti-β-Amyloid, 1-16 Antibody (Previously Covance catalog# SIG-39300)

Pricing & Availability
Clone
6E10 (See other available formats)
Regulatory Status
RUO
Other Names
AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously
Signet Catalog# 9300-02
Signet Catalog# 9300-05
Signet Catalog# 9300-10
Covance Catalog# SIG-39300
Isotype
Mouse IgG1, κ
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Product Citations
publications
A_6E10_ASCITES_betaAmyloid_1-16_WB_050818
Western blot of anti-β-amyloid, 1-16 antibody (clone 6E10). Lane 1: Molecular weight marker; Lane 2: 50 ng of the human Aβ1-40 peptide; Lane 3: 50 ng of the Aβ1-42 peptide; Lane 4: 50 ng of the recombinant human APP751 protein; Lane 5: 20 µg of the human brain lysate; Lane 6: 50 ng of the rodent Aβ1-42 peptide. The blot was incubated with 1:10,000 dilution of the primary antibody overnight at 4°C, followed by incubation with the HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A_6E10_ASCITES_betaAmyloid_1-16_WB_050818
    Western blot of anti-β-amyloid, 1-16 antibody (clone 6E10). Lane 1: Molecular weight marker; Lane 2: 50 ng of the human Aβ1-40 peptide; Lane 3: 50 ng of the Aβ1-42 peptide; Lane 4: 50 ng of the recombinant human APP751 protein; Lane 5: 20 µg of the human brain lysate; Lane 6: 50 ng of the rodent Aβ1-42 peptide. The blot was incubated with 1:10,000 dilution of the primary antibody overnight at 4°C, followed by incubation with the HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • B_6E10_ASCITES_betaAmyloid_1-16_DirectELISA_050818
    Direct ELISA of anti-β-amyloid, 1-16 (clone 6E10) antibody binding to the plate-immobilized human Aβ1-40, human Aβ1-42, rodent Aβ1-42 peptides, and recombinant human APP751 protein. ELISA was performed by coating the wells with 100 ng of peptide or recombinant protein. The wells were then incubated with the primary antibody at 37°C for 45 minutes, followed by incubation with HRP labeled goat anti-mouse IgG secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system.
  • C_6E10_ASCITES_betaAmyloid_1-16_IHCP_050818
    IHC staining of anti-β-Amyloid, 1-16 antibody (clone 6E10) on formalin-fixed paraffin-embedded normal human (left panel) and Alzheimer’s disease (right panel)brain tissues. Following antigen retrieval using 70% formic acid for 20 minutes, the tissues were incubated with 1:5,000 dilution of the primary antibody for 1 hour at room temperature. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining and bluing solution counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm
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803016 500 µL 2143 CHF
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803017 1 mL 3593 CHF
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803015 200 µL 1054 CHF
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803014 50 µL 300 CHF
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Description

Alzheimer's disease is characterized by the accumulation of aggregated Aβ peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor proteins (APP) through sequential proteolytic cleavage of APP by β-secretases and γ-secretases generating diverse Aβ species. Aβ can aggregate to form soluble oligomeric species and insoluble fibrillar or amorphous assemblies. Some forms of the aggregated peptides are toxic to neurons.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Preparation
Ascites
Concentration
The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
Storage & Handling
Store at -20°C or below. Upon initial thawing, apportion into working aliquots and store at -20°C or below. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Do not store in frost-free freezers.
Application

WB - Quality tested
Direct ELISA, IHC-P - Verified
IHC-F, EM - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution of this reagent is 1:1,000 to 1:20,000. For Direct ELISA, the suggested dilution of this reagent is 1:10,000 to 1:100,000. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, the suggested dilution of this reagent is 1:1,000 to 1:20,000. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is reactive to amino acid residue 1-16 of beta amyloid. The epitope lies within amino acids 3-8 of beta amyloid (EFRHDS).

This antibody clone has been reported for use in immunohistochemistry of free-floating sections2,13.

Application References

(PubMed link indicates BioLegend citation)
  1. Thakker DR, et al. 2009. Proc. Natl. Acad. Sci. USA. 106(11):4501-6. (IHC) PubMed
  2. Oddo S, et al. 2005. Proc. Natl. Acad. Sci. USA. 102(8):3046-51. (IHC-other) PubMed
  3. Herzig M, et al. 2004. Nat. Neuro. 7(9):954-959. (WB) PubMed
  4. Zheng Y, et al. 2012. PLoS One 6:39035. (IHC-F) PubMed
  5. Abramowksi D, et al. J Neurosci. 32:1273. (WB) PubMed
  6. Forny-Germano L, et al. 2014. J. Neurosci. 34:13629. (WB, IHC) PubMed
  7. Gowert NS, et al. 2014. PLoS One 2:e90523. (ICC, EM) PubMed
  8. Sandoval-Hernández A, et al. 2015. PLoS One. 10: 0145467. (IHC-F)
  9. Kumar R, et al. 2016. Brain. 139:174-92 (WB)
  10. Miyamoto T, et al. 2016. J. Biol. Chem. 291:1719-34. (WB)
  11. Saito S, et al. 2017. Acta Neuropathol. Commun. 5:26-9. (IHC-P) PubMed
  12. Omata Y, et al. 2016. Aging (Albany NY) 8(3):427. (IHC-P) PubMed
  13. Peng W, et al. 2016. Neurobiol. Dis. 93:215. (IHC-other) PubMed
  14. Mandler M, et al. 2015. PLoS One. e0115237. (WB, IHC, ELISA) PubMed
Product Citations
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  25. Forny-Germano L, et al. 2014. J Neurosci. 34:13629-13643. PubMed
  26. Gowert N, et al. 2014. PLoS One. 9:e90523. PubMed
  27. Abramowski D, et al. 2012. J Neurosci. 32:1273-1283. PubMed
  28. Chan E, et al. 2016. Sci Rep. 6: 26119. PubMed
  29. Heiss J, et al. 2016. Cereb Cortex. 10.1093/cercor/bhw188. PubMed
  30. Li T, et al. 2016. Nat Commun. 7:12082. PubMed
  31. Fujita K, et al. 2016. Sci Rep. 6:31895. PubMed
  32. Beraldo F, et al. 2016. J Biol Chem. 291: 21945 - 21955. PubMed
  33. Zhang H, et al. 2016. J Neurosci. 36: 11837 - 11850. PubMed
  34. Scholtzova H, et al. 2017. J Neurosci. 37(4):936-959. PubMed
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  37. Arber C, et al. 2020. Mol Psychiatry. 25:2919. PubMed
  38. Quartey MO, et al. 2021. Sci Rep. 0.757638889. PubMed
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  44. Casali BT, et al. 2020. Neurobiol Dis. 142:104956. PubMed
  45. Zhang J, et al. 2021. Aging Cell. 20:e13380. PubMed
  46. Ye T, et al. 2021. Commun Biol. 4:195. PubMed
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  48. Ge W, et al. 2021. Am J Transl Res. 13:1471. PubMed
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  57. Ren X, et al. 2022. J Neuroinflammation. 19:61. PubMed
  58. Shen Q, et al. 2022. Aging Cell. 21:e13573. PubMed
  59. Lai RH, et al. 2021. Aging Cell. 20:e13509. PubMed
  60. Lin H, et al. 2022. Front Aging Neurosci. 14:885145. PubMed
  61. Peng D, et al. 2022. Theranostics. 12:3862. PubMed
  62. Kaur H, et al. 2021. Bioorg Med Chem Lett. 46:128164. PubMed
  63. Cone AS, et al. 2021. Theranostics. 11:8129. PubMed
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  67. Qiu Y, et al. 2022. Front Aging Neurosci. 14:896522. PubMed
  68. Shang Y, et al. 2022. Acta Neuropathol. 144:911. PubMed
  69. Lai RH, et al. 2022. Aging Cell. 21:e13670. PubMed
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  72. Januário YC, et al. 2022. J Biol Chem. 298:102172. PubMed
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  77. Bathini P, et al. 2023. Brain Behav Immun Health. 28:100568. PubMed
  78. Lin W, et al. 2023. J Neuroinflammation. 20:21. PubMed
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RRID
AB_2565329 (BioLegend Cat. No. 803016)
AB_2565327 (BioLegend Cat. No. 803017)
AB_2565328 (BioLegend Cat. No. 803015)
AB_2728527 (BioLegend Cat. No. 803014)

Antigen Details

Structure
Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.
Distribution

Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, muscle.
Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.

Function
The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Antigen References
  1. Kumar A, et al. 2015. Pharmacol. Rep. 67(2):195.
  2. Sadigh-Eteghad S, et al. 2015. Med. Princ. Pract. 24(1):1
  3. Hampel H, et al. 2015. Expert Rev. Neurother. 15(1):83.
  4. Puig KL, et al. 2012.  Exp. Gerontol. 48(7): 608.
  5. Selkoe DJ, et al. 2016. EMBO Mol. Med. 8(6):595.
  6. Walsh DM, et al.  2007. J. Neurochem. 101(5):1172.
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-Amyloid 1-16 on UniProt.org
Go To Top Version: 4    Revision Date: 03.12.2021

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