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APC anti-human CD11b Antibody

Pricing & Availability
Clone
ICRF44 (See other available formats)
Regulatory Status
RUO
Workshop
IV M047
Other Names
Integrin αM chain, C3biR, CR3, Mac-1, Mo1, ITGAM
Isotype
Mouse IgG1, κ
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Product Citations
publications
ICRF44_APC_061407
Human peripheral blood granulocytes stained with ICRF44 APC
  • ICRF44_APC_061407
    Human peripheral blood granulocytes stained with ICRF44 APC
See APC spectral data
Cat # Size Price Quantity Check Availability Save
301309 25 tests 44 CHF
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301310 100 tests 132 CHF
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301350 100 µg 296 CHF
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Description

CD11b is a 165-170 kD type I transmembrane glycoprotein also known as αM integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin β2 (CD18) and is expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils. It is also involved in granulocyte adhesion, phagocytosis, and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, and factor X.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The ICRF44 antibody inhibits heterotypic adhesion of granulocytes in response to fMLP. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, immunofluorescence microscopy5, stimulation of monocytes3, blocking of heterotypic PMN aggregation8, and blocking of granulocyte activation12. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 301361 & 301362).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  2. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  3. Rezzonico R, et al. 2001. Blood 97:2932. (Stim)
  4. Marsik C, et al. 2003. Shock 20:493. (FC)
  5. David A, et al. 2003. J. Leukoc. Biol. 74:551. (IF)
  6. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  7. Thurlow LR, et al. 2010. Infect. Immun. 128:1128. (FC) PubMed
  8. Jadhav S, et al. 2001. J. Immunol. 167:5986. (Block)
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  10. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21. (FC)
  11. Wen T, et al. 2014. J Immunol. 192:5481. (FC) PubMed
  12. Sprong T, et al. 2003. Blood 102:3702. (Block)
  13. Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed
  14. Larsson K, et al. 2015. PNAS. PubMed
Product Citations
  1. Hazeldine J, et al. 2017. PLoS Med. 10.1371/journal.pmed.1002338. PubMed
  2. Surdziel E, et al. 2017. PLoS One.. 10.1371/journal.pone.0183679. PubMed
  3. Halim TYF et al. 2018. Immunity. 48(6):1195-1207 . PubMed
  4. Saggu G, et al. 2018. Nat Commun. 9:5058. PubMed
  5. Jardine L, et al. 2019. Nat Commun. 10:1999. PubMed
  6. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  7. Morton JJ, et al. 2018. Mol Carcinog. 57:1651. PubMed
  8. Velázquez–Avila M, et al. 2019. Leukemia. 33:1337. PubMed
  9. Horn LA, et al. 2017. Oncotarget. 8:57964. PubMed
  10. Dan H, et al. 2020. Mol Oncol. 14:795. PubMed
  11. Eriksson E, et al. 2019. J Immunol. 202:787. PubMed
  12. Zhai Y, et al. 2018. Autophagy. 1.714583333. PubMed
  13. Thurlow L, et al. 2010. Infect Immun. 78:4936. PubMed
  14. Berger M, et al. 2012. J Biol Chem. 287:19409. PubMed
  15. Sun Z, et al. 2013. PLoS One. 8:74672. PubMed
  16. André V, et al. 2012. Blood Cancer J. 2:94. PubMed
  17. Hünniger K, et al. 2015. J Immunol. 194:1199. PubMed
  18. Bigley V, et al. 2015. J Leukoc Biol. 97:627. PubMed
  19. Guo X, et al. 2016. Mol Ther Methods Clin Dev. 3:15054. PubMed
  20. Sade-Feldman M, et al. 2016. Clin Cancer Res. 22(23):5661-5672. PubMed
  21. Julian Buchrieser, William James, Michael D. Moore 2017. Stem Cell Reports. 8(2):334-345. PubMed
  22. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  23. Wang E, et al. 2021. Cell Stem Cell. 28(4):718-731.e6. PubMed
  24. Richardson SE, et al. 2021. STAR Protocols. 2(2):100420. PubMed
  25. Johnston G, et al. 2020. Gene. 752:144758. PubMed
  26. Fu Y, et al. 2021. Cell Reports. 36(2):109344. PubMed
  27. Hemkemeyer SA, et al. 2020. J Biol Chem. . PubMed
  28. Kiehlmeier S, et al. 2021. Leukemia. Online ahead of prin. PubMed
  29. Ren X, et al. 2021. Cell Death Dis. 12:484. PubMed
  30. Wegner J, et al. 2021. Sci Rep. 11:14983. PubMed
  31. Szentkereszty M, et al. 2020. Pathol Oncol Res. 26:1117. PubMed
  32. Krampert L, et al. 2021. Front Immunol. 12:712948. PubMed
  33. Schwabenland M, et al. 2021. Acta Neuropathol. 142:923. PubMed
  34. Tripathi H, et al. 2020. J Mol Cell Cardiol. 149:95. PubMed
  35. Perelman SS, et al. 2021. Nat Microbiol. 6:731. PubMed
  36. Yang E, et al. 2021. Exp Mol Med. 53:1902. PubMed
  37. Li Y, et al. 2022. Cell Rep. 38:110284. PubMed
  38. Qiu X, et al. 2022. Cell. 185:690. PubMed
  39. Schimek V, et al. 2022. Cell Death Dis. 13:113. PubMed
  40. Hemkemeyer SA, et al. 2020. J Biol Chem. 296:100136. PubMed
  41. Konduru NV, et al. 2021. Nanotheranostics. 5:309. PubMed
  42. Luque-Martin R, et al. 2021. J Immunol. 207:555. PubMed
  43. Collado A, et al. 2021. Front Pharmacol. 12:656244. PubMed
  44. Wu X, et al. 2022. Front Immunol. 13:849493. PubMed
  45. Zelter T, et al. 2022. EMBO Rep. 23:e53641. PubMed
  46. Song M, et al. 2022. J Gene Med. 24:e3455. PubMed
  47. LaSalle TJ, et al. 2022. Cell Rep Med. 3:100779. PubMed
  48. Rodionov G, et al. 2022. Bone Marrow Transplant. 57:1250. PubMed
  49. Tessaro FHG, et al. 2022. Cell Rep. 39:110977. PubMed
  50. Zhang W, et al. 2022. Dev Cell. 57:329. PubMed
  51. Olsen SN, et al. 2022. Mol Cell. 82:1140. PubMed
  52. Lee CW, et al. 2022. J Adv Res. 39:73. PubMed
  53. Mauracher LM, et al. 2023. Res Pract Thromb Haemost. 7:100126. PubMed
RRID
AB_314161 (BioLegend Cat. No. 301309)
AB_314162 (BioLegend Cat. No. 301310)
AB_2564134 (BioLegend Cat. No. 301350)

Antigen Details

Structure
Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 165-170 kD
Distribution

Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subset of T cells, subset of B cells

Function
Adhesion, phagocytosis, chemotaxis, neutrophil activation
Ligand/Receptor
ICAM-1(CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Stewart M, et al. 1995. Curr Opin Cell Biol. 7:690.

Gene ID
3684 View all products for this Gene ID
UniProt
View information about CD11b on UniProt.org
Go To Top Version: 4    Revision Date: 07.13.2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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