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APC anti-human CD279 (PD-1) Antibody

Pricing & Availability
Clone
EH12.2H7 (See other available formats)
Regulatory Status
RUO
Other Names
PD-1, PDCD1
Isotype
Mouse IgG1, κ
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Product Citations
publications
EH12dot2H7_APC_022508
PHA-stimulated (day-3) human peripheral blood lymphocytes were stained with CD279 (clone EH12.2H7) APC (filled histogram) or mouse IgG1, κ APC (open histogram).
  • EH12dot2H7_APC_022508
    PHA-stimulated (day-3) human peripheral blood lymphocytes were stained with CD279 (clone EH12.2H7) APC (filled histogram) or mouse IgG1, κ APC (open histogram).
  • EH12dot2H7_APC_072209.jpg
    Human peripheral blood lymphocytes were stained with CD279 (clone EH12.2H7) APC and CD3 (clone UCHT1) PerCP/Cy5.5.
See APC spectral data
Cat # Size Price Quantity Check Availability Save
329907 25 tests 149 CHF
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329908 100 tests 314 CHF
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Description

Programmed cell death 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1) and PD-L2 (B7-DC) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of ligand binding1-3, immunohistochemical staining of paraformaldehyde fixed frozen sections13, and spatial biology (IBEX)15,16. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329911 and 329912). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 329926) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Dorfman DM, et al. 2006 Am. J. Surg. Pathol. 30:802. (FA)
  2. Radziewicz H, et al. 2007. J. Virol. 81:2545. (FA)
  3. Velu V, et al. 2007. J. Virol. 81:5819. (FA)
  4. Zahn RC, et al. 2008. J. Virol. 82:11577. PubMed
  5. Chang WS, et al. 2008. J. Immunol. 181:6707. (FC) PubMed
  6. Nakamoto N, et al. 2009. PLoS Pathog. 5:e1000313. (FA)
  7. Jones RB, et al. 2009. J. Virol. 83:8722. (FC) PubMed
  8. Vojnov L, et al. 2010. J. Virol. 84:753. (FC) PubMed
  9. Radziewicz H, et al. 2010. J. Immunol. 184:2410. (FC) PubMed
  10. Monteriro P, et al. 2011. J. Immunol. 186:4618. PubMed
  11. Conrad J, et al. 2011. J. Immunol. 186:6871. PubMed
  12. Salisch NC, et al. 2010. J. Immunol. 184:476. (Rhesus reactivity)
  13. Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)
  14. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  15. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  16. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Lecciso M, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01918. PubMed
  2. Wang Z, et al. 2018. Nat Commun. 9:824. PubMed
  3. Niu C, et al. 2018. Front Immunol. 9:1759. PubMed
  4. Frasca D, et al. 2018. PLoS One. 13:e0197472. PubMed
  5. Liu Y, et al. 2018. Cancer Cell. 33:480. PubMed
  6. Zhao Y et al. 2018. Cell reports. 24(2):379-390 . PubMed
  7. Kraig E, et al. 2018. Exp Gerontol. 105:53. PubMed
  8. Wei J, et al. 2019. J Immunother Cancer. 7:209. PubMed
  9. Zou F, et al. 2019. Nat Commun. 10:4109. PubMed
  10. Martín–Gayo E, et al. 2020. Cell Rep. 30:984. PubMed
  11. Renner K, et al. 2020. Cell Reports. 29(1):135-150.e9.. PubMed
  12. Zhu Y, et al. 2019. Cell Stem Cell. 25:542. PubMed
  13. Schultheiß C, et al. 2020. Immunity. 53(2):442-455.e4.. PubMed
  14. Xu H, et al. 2013. J Leukoc Biol. 93:943. PubMed
  15. Tian X, et al. 2015. J Immunol. 194:3873. PubMed
  16. Blackburn M, et al. 2015. J Immunol. 195: 3227 - 3236. PubMed
  17. Miles B, et al. 2015. Nat Commun. 6: 8608. PubMed
  18. Javed A, et al. 2015. PLoS One. 10: 0142086. PubMed
  19. Chew G, et al. 2016. PLoS Pathog. 12: 1005349. PubMed
  20. Beyer M, et al. 2016. Nat Immunol. 17:593-603. PubMed
  21. Miles B, et al. 2016. PLoS Pathog. 12:e1005924. PubMed
  22. Montes de Oca M, et al. 2016. Cell Rep. 17:399-412. PubMed
  23. Wang D, et al. 2017. Leukemia. 10.1038/leu.2017.69. PubMed
  24. Chan JA, et al. 2020. Cell Rep Med. 1:100157. PubMed
  25. Rodda LB, et al. 2020. Cell. 184(1):169-183.e17. PubMed
  26. Wang S, et al. 2021. Exp Ther Med. 21:37. PubMed
  27. Zhang Y, et al. 2020. Oncol Lett. 1.053472222. PubMed
  28. Tan EE, et al. 2020. J Clin Invest. 130:5817. PubMed
  29. Cai J, et al. 2021. eLife. 10:00. PubMed
  30. Todorova D, et al. 2020. EBioMedicine. 62:103120. PubMed
  31. Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed
  32. Ng KW, et al. 2019. eLife. 0.333333333333333. PubMed
  33. Frasca D, et al. 2021. Front Immunol. 616650:12. PubMed
  34. Huang Y, et al. 2020. FASEB J. 34:1768. PubMed
  35. Wei JL, et al. 2021. J Immunother Cancer. 9: . PubMed
  36. Paul S, et al. 2021. Sci Transl Med. 13:. PubMed
  37. Panwar B, et al. 2021. Genome Res. 31:659. PubMed
  38. Porsche CE, et al. 2021. JCI Insight. 6:. PubMed
  39. Jia X, et al. 2021. Clin Transl Immunology. 10:e1336. PubMed
  40. Cheng L, et al. 2021. Cancer Immunol Immunother. Online ahead of print. PubMed
  41. Daugan MV, et al. 2021. Cancer Immunol Res. 9:891. PubMed
  42. Hirama T, et al. 2021. JCI Insight. 6:. PubMed
  43. Liao JB, et al. 2021. J Immunother Cancer. 9:. PubMed
  44. Zhang J, et al. 2022. Nature. 609:369. PubMed
  45. Yamaguchi A, et al. 2021. J Thorac Dis. 13:5430. PubMed
  46. Rasmussen TA, et al. 2022. Cell Rep Med. 3:100766. PubMed
  47. Cao B, et al. 2022. Nat Commun. 13:6203. PubMed
  48. Xu C, et al. 2022. Cell Mol Gastroenterol Hepatol. 15:327. PubMed
  49. Gao Y, et al. 2023. JCI Insight. 8: . PubMed
  50. Babl N, et al. 2023. Front Oncol. 13:1107484. PubMed
  51. Cheng K, et al. 2023. Int J Mol Sci. 24:. PubMed
  52. Segaliny AI, et al. 2023. Commun Biol. 6:380. PubMed
  53. Shi J, et al. 2023. Front Immunol. 14:1166052. PubMed
  54. Menevse AN, et al. 2023. Acta Neuropathol Commun. 11:75. PubMed
  55. Imai H, et al. 2023. iScience. 26:106822. PubMed
RRID
AB_940473 (BioLegend Cat. No. 329907)
AB_940475 (BioLegend Cat. No. 329908)

Antigen Details

Structure
Immunoglobulin superfamily
Distribution

Transiently expressed on CD4- CD8- thymocytes; upregulated in thymocytes and splenic T and B lymphocytes; expressed on activated myeloid cells

Ligand/Receptor
B7-H1 (also known as PD-L1) and B7-DC (PD-L2)
Cell Type
B cells, Lymphocytes, T cells, Thymocytes, Tregs
Biology Area
Cancer Biomarkers, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Gene ID
5133 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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