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APC anti-human IL-13 Antibody

Pricing & Availability
Clone
JES10-5A2 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-13, NC30
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
JES10-5A2_APC_A_030408
Enriched human CD4+ T cells were stimulated with PMA+ionomycin, then surface stained with CD4 PE/Cy5 and intracellular stained with APC rat IgG1,k isotype control (top) or APC JES10-5A2 (bottom)
  • JES10-5A2_APC_A_030408
    Enriched human CD4+ T cells were stimulated with PMA+ionomycin, then surface stained with CD4 PE/Cy5 and intracellular stained with APC rat IgG1,k isotype control (top) or APC JES10-5A2 (bottom)
  • JES10-5A2_APC_B_030408
    Enriched human CD4+ T cells were stimulated with PMA+ionomycin, then surface stained with CD4 PE/Cy5 and intracellular stained with APC rat IgG1,k isotype control (top) or APC JES10-5A2 (bottom)
See APC spectral data
Cat # Size Price Quantity Check Availability Save
501908 50 µg 172 CHF
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501907 100 tests 343 CHF
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Description

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 lymphocytes. IL-13 shares 30% amino acid sequence homology with IL-4 and demonstrates similar biological activities. The biological activities of IL-13 include: suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression, and suppression of proinflammatory cytokine secretion. The JES10-5A2 antibody reacts with human interleukin-13 (IL-13). The JES10-5A2 antibody can neutralize the bioactivity of natural or recombinant IL-13.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
COS-expressed, recombinant human IL-13
Formulation
test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

ELISA or ELISPOT Capture1,2: The Ultra-LEAF™ Purified JES10-5A2 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5020 antibody (Cat. No. 502001) as the detecting antibody. The Ultra-LEAF™ Purified antibody is suggested for ELISPOT capture.

Application References

(PubMed link indicates BioLegend citation)
  1. McKenzie, A., et al. 1994. Curr. Prot. Immunol.. John Wiley & Sons. New York. Section 6.18.
  2. Abrams, J. 1995. Curr. Prot. Immunol.. John Wiley and Sons, New York. Unit 6.20.
  3. Schaerli, P., et al. 2000. J. Exp. Med. 192:1553.
  4. Andersson, J., et al. 1994. Immunology 83:16.
  5. Skansen-Saphir, U., et al. 1994. Eur. J. Immunol. 24:916.
  6. Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
  7. Gelfand, E. W., et al. 2006. J. Aller. and Clinic. Immunol. 117:577.
  8. Knorr, C., et al. 1999. Am. J. Pathol. 155:2019.
Product Citations
  1. Chen L et al. 2018. Immunity. 49(3):464-476 . PubMed
  2. Arif S, et al. 2020. Diabetologia. 63(6):1186-1198.. PubMed
  3. Huber J, et al. 2010. J Immunol. 185:813. PubMed
  4. Neunkirchner A, et al. 2011. J Immunol. 187:4077. PubMed
  5. Barber J, et al. 2013. Proc Natl Acad Sci U S A. 110:8164. PubMed
  6. Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed
  7. Vacaflores A, et al. 2016. PLoS One. 11: 0157175. PubMed
  8. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
  9. Yu KK, et al. 2021. JCI Insight. 6:. PubMed
  10. Corbière V, et al. 2023. Front Immunol. 14:1101366. PubMed
RRID
AB_315203 (BioLegend Cat. No. 501908)
AB_315202 (BioLegend Cat. No. 501907)

Antigen Details

Structure
Cytokine; 17 kD (Mammalian)
Bioactivity
Suppression of macrophage cytotoxic activity; upregulation of IL-1RA expression; suppression of IL-1, IL-6, IL-8, IL-10, IL-12, chemokines MCP, MIP-1; differentiation of B cells, monocytes
Cell Sources
Activated T cells, mast cells, NK cells
Cell Targets
B cells, monocytes, macrophages, basophils
Receptors
Moderate affinity IL-13Rα1; high affinity heterodimer IL-13Rα1/IL-4Rα also binds IL-4; both receptor complexes have γ-subunit in common with IL-2R, IL-4R, IL-7R, IL-15R ; high affinity IL-13Rα2 incapable of signaling
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Hilton, D., et al. 1996. P. Natl. Acad. Sci. USA 93:497.
3. Obiri, N., et al. 1995. J. Biol. Chem. 270:8797.
4. Ochensberger, B., et al. 1996. Blood 88:3028.

Regulation
IL-13Rα2 possible antagonist
Gene ID
3596 View all products for this Gene ID
UniProt
View information about IL-13 on UniProt.org

Related FAQs

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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