APC anti-mouse IgM Antibody

Product Details

Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse Ig cocktail

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Red Laser (633 nm)

Application Notes

The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.

Application References

(PubMed link indicates BioLegend citation)

1. Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed

Product Citations

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24. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
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28. Leung G, et al. 2021. JCI Insight. 6:. PubMed
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32. Herrejon Chavez F, et al. 2023. Nat Commun. 14:2290. PubMed

RRID

AB_315059 (BioLegend Cat. No. 406509)

Antigen Details

Structure

Ig family

Distribution

B cells

Function

B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis

Cell Type

B cells

Biology Area

Immunology

Gene ID

16019 View all products for this Gene ID

UniProt

View information about IgM on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Other Formats

Concentration & Expiration Lookup

Certificate of Analysis