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APC/Cyanine7 anti-human CD279 (PD-1) Antibody

Pricing & Availability
Clone
EH12.2H7 (See other available formats)
Regulatory Status
RUO
Other Names
PD-1, PDCD1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
EH12dot2H7_APCCyanine7_CD279_Antibody_031919_updated.png
PHA-stimulated (day-3) human peripheral blood lymphocytes stained with CD279 (PD-1) (clone EH12.2H7) APC/Cyanine7 (filled histogram) or Mouse IgG1, κ APC/Cyanine7 isotype control (open histogram)
  • EH12dot2H7_APCCyanine7_CD279_Antibody_031919_updated.png
    PHA-stimulated (day-3) human peripheral blood lymphocytes stained with CD279 (PD-1) (clone EH12.2H7) APC/Cyanine7 (filled histogram) or Mouse IgG1, κ APC/Cyanine7 isotype control (open histogram)
See APC/Cyanine7 spectral data
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329921 25 tests 176 CHF
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329922 100 tests 382 CHF
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Description

Programmed cell death 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1) and PD-L2 (B7-DC) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of ligand binding1-3, immunohistochemical staining of paraformaldehyde fixed frozen sections13, and spatial biology (IBEX)15,16. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329911 and 329912). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 329926) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Dorfman DM, et al. 2006 Am. J. Surg. Pathol. 30:802. (FA)
  2. Radziewicz H, et al. 2007. J. Virol. 81:2545. (FA)
  3. Velu V, et al. 2007. J. Virol. 81:5819. (FA)
  4. Zahn RC, et al. 2008. J. Virol. 82:11577. PubMed
  5. Chang WS, et al. 2008. J. Immunol. 181:6707. (FC) PubMed
  6. Nakamoto N, et al. 2009. PLoS Pathog. 5:e1000313. (FA)
  7. Jones RB, et al. 2009. J. Virol. 83:8722. (FC) PubMed
  8. Vojnov L, et al. 2010. J. Virol. 84:753. (FC) PubMed
  9. Radziewicz H, et al. 2010. J. Immunol. 184:2410. (FC) PubMed
  10. Monteriro P, et al. 2011. J. Immunol. 186:4618. PubMed
  11. Conrad J, et al. 2011. J. Immunol. 186:6871. PubMed
  12. Salisch NC, et al. 2010. J. Immunol. 184:476. (Rhesus reactivity)
  13. Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)
  14. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  15. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  16. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Perrot I et al. 2019. Cell Rep. 27(8):2411-2425 . PubMed
  2. Andersch L, et al. 2019. BMC Cancer. 19:895. PubMed
  3. Linedale R, et al. 2017. PLoS One. 12:e0175755. PubMed
  4. Wang J, et al. 2019. Oncol Lett. 4.948611111. PubMed
  5. Yost KE, et al. 2019. Nat Med. 25:1251. PubMed
  6. Fisher J, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.03.002. PubMed
  7. Toews K, et al. 2020. Mol Carcinog. 724:59. PubMed
  8. Zhan Y, et al. 2021. JCI Insight. 6:. PubMed
  9. Rascle P, et al. 2021. iScience. 24:103109. PubMed
  10. Klaver Y, et al. 2020. J Immunother Cancer. 8:. PubMed
  11. Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed
  12. Eiva MA, et al. 2022. Eur J Immunol. 52:96. PubMed
  13. Hu M, et al. 2022. Int J Mol Sci. 23: . PubMed
  14. Ogura H, et al. 2022. Nat Commun. 13:7063. PubMed
  15. Pandit H, et al. 2023. iScience. 26:105929. PubMed
RRID
AB_10900982 (BioLegend Cat. No. 329921)
AB_10933429 (BioLegend Cat. No. 329922)

Antigen Details

Structure
Immunoglobulin superfamily
Distribution

Transiently expressed on CD4- CD8- thymocytes; upregulated in thymocytes and splenic T and B lymphocytes; expressed on activated myeloid cells

Ligand/Receptor
B7-H1 (also known as PD-L1) and B7-DC (PD-L2)
Cell Type
B cells, Lymphocytes, T cells, Thymocytes, Tregs
Biology Area
Cancer Biomarkers, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Gene ID
5133 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org
Go To Top Version: 4    Revision Date: 03.19.2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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