- Clone
- AK4 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- VI P-44
- Other Names
- GMP-140, PADGEM
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![AK4_APCCyanine7_CD62P_Antibody_041219.png AK4_APCCyanine7_CD62P_Antibody_041219.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/AK4_APCCyanine7_CD62P_Antibody_041219.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Thrombin-activated human peripheral blood platelets were stained with CD62P (P-Selectin) (clone AK4) APC/Cyanine7 (filled histogram) or mouse IgG1, κ APC/Cyanine7 isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
304943 | 25 tests | 141 CHF | ||||
304944 | 100 tests | 352 CHF |
CD62P is a 140 kD type I transmembrane glycoprotein also known as P-selectin, platelet activation-dependent granule membrane protein (PADGEM), and GMP-140. It is expressed on activated platelets, megakaryocytes, and endothelial cells. CD62P is primarily stored in secretory α-granules in platelets and Weibel-Palade bodies in endothelial cells, and is rapidly relocated to the plasma membrane upon activation. The ligands for CD62P are CD162 and CD24. A primary function of CD62P is cell adhesion during neutrophil rolling, and platelet-neutrophil and platelet-monocyte interactions.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
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Red Laser (633 nm)
- Application Notes
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Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections4 and in vitro blocking of adhesion of platelets1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 304947 & 304948).
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Application References
(PubMed link indicates BioLegend citation) -
- Skinner M, et al. 1991. J. Biol. Chem. 266:5371. (Block)
- Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
- Yen YT, et al. 2006. J. Virol. 80:2684.
- Sato Y, et al. 2005. Blood 106:428. (IHC)
- RRID
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AB_2810453 (BioLegend Cat. No. 304943)
AB_2810454 (BioLegend Cat. No. 304944)
Antigen Details
- Structure
- Selectin, type I glycoprotein, 140 kD
- Distribution
-
Activated platelets, megakaryocytes, endothelial cells
- Function
- Adhesion, neutrophil rolling, platelet-neutrophil and platelet-monocyte interactions
- Ligand/Receptor
- CD162 (PSGL-1), CD24 and sialylated Lewis X
- Cell Type
- Endothelial cells, Megakaryocytes, Neutrophils, Platelets
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Neuroscience, Synaptic Biology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. McEver R, et al. 1995. J. Biol. Chem. 270:11025.
2. Varki A. 1994. P. Natl. Acad. Sci. USA 91:7390. - Gene ID
- 6403 View all products for this Gene ID
- UniProt
- View information about CD62P on UniProt.org
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