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Biotin anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
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Product Citations
publications
6D5_Biotin_080207
C57BL/6 mouse splenocytes were stained with biotinylated CD19 (clone 6D5) (filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by Sav-PE.
  • 6D5_Biotin_080207
    C57BL/6 mouse splenocytes were stained with biotinylated CD19 (clone 6D5) (filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by Sav-PE.
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115503 50 µg 54 CHF
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115504 500 µg 134 CHF
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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Application References

(PubMed link indicates BioLegend citation)
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Joachim R, Suber F, and Kobzik L 2017. Sci Rep. . 10.1038/s41598-017-16743-1. PubMed
  2. Tanimura N et al. 2018. Developmental cell. 46(5):581-594 . PubMed
  3. Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
  4. Macal M et al. 2018. Immunity. 48(4):730-744 . PubMed
  5. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  6. Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed
  7. Kimball A, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1102. PubMed
  8. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  9. Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
  10. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  11. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  12. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  13. Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed
  14. Blecher-Gonen R, et al. 2019. Cell Syst. 8:109. PubMed
  15. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
  16. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  17. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  18. Akkaya B, et al. 2017. J Immunol Methods. 441:67. PubMed
  19. Mitsumura T, et al. 2018. Blood Adv. 2.502083333. PubMed
  20. O'Boyle C, et al. 2020. Int J Stroke. 0.746527778. PubMed
  21. Park DS, et al. 2019. PLoS Comput Biol. 15:e1006913. PubMed
  22. von Gamm M, et al. 2019. J Exp Med. 216:1700. PubMed
  23. Khaw YM, et al. 2020. J Neuroinflammation. 17:49. PubMed
  24. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  25. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  26. Au-Yeung BB, et al. 2017. J Immunol. 198:2445. PubMed
  27. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  28. Ji Y, et al. 2019. Nat Immunol. 1.303472222. PubMed
  29. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  30. Niss K, et al. 2020. Cell Reports. 31(11):107763. PubMed
  31. Kunimoto H, et al. 2012. Sci Rep. 0.272916667. PubMed
  32. Datta S, et al. 2014. J Leukoc Biol. 95:853. PubMed
  33. Adachi Y, et al. 2015. J Exp Med. 212: 1709-1723. PubMed
  34. Olsson A, et al. 2016. Nature. 10.1038/nature19348. PubMed
  35. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  36. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  37. Laubreton D, et al. 2020. Viruses. 12:00. PubMed
  38. Liu YY, et al. 2020. Chin Med J (Engl). 133:1688. PubMed
  39. Kimball AS, et al. 2020. Thromb Haemost. 120:289. PubMed
  40. Geiger SS, et al. 2021. Nature Communications. 12(1):2745. PubMed
  41. Milner JJ, et al. 2020. Immunity. 52(5):808-824.e7. PubMed
  42. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  43. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  44. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  45. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  46. Davis FM, et al. 2020. J Immunol. 2503:204. PubMed
  47. Hutter K, et al. 2020. FEBS J. . PubMed
  48. Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed
  49. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  50. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  51. Tsyklauri O, et al. 2021. EMBO Rep. 22:e50785. PubMed
  52. Dong S, et al. 2021. Nature. 591:117. PubMed
  53. Hoffmann A, et al. 2021. EBioMedicine. 71:103568. PubMed
  54. Rødahl I, et al. 2021. STAR Protoc. 2:100842. PubMed
  55. Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed
  56. Davis FM, et al. 2021. J Exp Med. 218:. PubMed
  57. Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed
  58. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  59. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  60. She L, et al. 2021. JCI Insight. 6:e143509. PubMed
  61. Zhou Z, et al. 2022. Infect Immun. 90:e0045321. PubMed
  62. Sasse C, et al. 2022. Pathogens. 11:. PubMed
  63. Tian Q, et al. 2021. Infect Immun. 89:e0020521. PubMed
  64. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  65. Wolf SJ, et al. 2022. JCI Insight. 7:. PubMed
  66. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  67. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  68. Song R, et al. 2022. Immunology. 165:274. PubMed
  69. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  70. Chanda MK, et al. 2022. J Immunol Methods. 506:113290. PubMed
  71. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  72. Koizumi M, et al. 2022. J Biol Chem. 298:102506. PubMed
  73. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  74. Giannou AD, et al. 2023. Immunity. 56:125. PubMed
  75. Khaw YM, et al. 2023. EMBO Rep. 24:e54228. PubMed
  76. Gonçalves R, et al. 2023. iScience. 26:105972. PubMed
  77. Agrafiotis A, et al. 2023. Acta Neuropathol. 145:335. PubMed
  78. Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed
  79. Friedman D, et al. 2022. J Immunol. 208:1845. PubMed
  80. Obi AT, et al. 2023. Sci Rep. 13:3253. PubMed
  81. Xu H, et al. 2023. Elife. 12:. PubMed
  82. Miyake K, et al. 2023. Nat Commun. 14:2694. PubMed
RRID
AB_313638 (BioLegend Cat. No. 115503)
AB_313639 (BioLegend Cat. No. 115504)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 05.24.2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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