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Brilliant Violet 421™ anti-human CD45RA Antibody

Pricing & Availability
Clone
HI100 (See other available formats)
Regulatory Status
RUO
Workshop
IV N906
Other Names
GP180, L-CA, LCA, LY5, T200, PTPRC
Isotype
Mouse IgG2b, κ
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Product Citations
publications
1_HI100_BV421_1_040411
Human peripheral blood lymphocytes were stained with CD45RO FITC and CD45RA (clone HI100) Brilliant Violet 421™ (top) or mouse IgG2b, κ Brilliant Violet 421™ isotype control (bottom).
  • 1_HI100_BV421_1_040411
    Human peripheral blood lymphocytes were stained with CD45RO FITC and CD45RA (clone HI100) Brilliant Violet 421™ (top) or mouse IgG2b, κ Brilliant Violet 421™ isotype control (bottom).
  • 2_HI100_BV421_2_040411
  • 3_HI100_BV421_CD45RA_Antibody_IHC-P_010318
    Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate-Buffered 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes, permeabilized with 0.5% Tween 20 in PBS and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of Alexa Fluor® 594 anti-human CD45RO (clone UCHL1) antibody (red) and Brilliant Violet 421™ anti-human CD45RA (clone HI100) antibody (blue) overnight at 4°C. The image was taken with a 10X objective.
See Brilliant Violet 421™ spectral data
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304129 25 tests 176 CHF
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304130 100 tests 380 CHF
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Description

CD45RA is a 205-220 kD single chain type I glycoprotein. It is an exon 4 splice variant of the tyrosine phosphatase CD45. The CD45RA isoform is expressed on resting/naïve T cells, medullary thymocytes, B cells and monocytes. CD45RA enhances both T cell receptor and B cell receptor signaling. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for relevant formats of this clone) include: inhibition of CD45 functions2, immunohistochemical staining of frozen tissue sections3 and formalin-fixed paraffin-embedded tissue sections4, and immunocytochemistry15,16.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Yamada T, et al. 2002. J. Biol. Chem. 277:28830. (WB, Block)
  3. Weninger W, et al. 2003 J. Immunol. 170:4638. (IHC-F)
  4. Imanguli MM, et al. 2009. Blood. 113:3620 (IHC-P)
  5. Roque S, et al. 2007. J. Immunol. 178:8028. (FC) PubMed
  6. Smeltz RB. 2007. J. Immunol. 178:4786. (FC) PubMed
  7. Palendira U, et al. 2008. Blood (FC) PubMed
  8. Kuttruff S, et al. 2009. Blood 113:358. (FC) PubMed
  9. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  10. Alanio C, et al. 2010. Blood 115:3718. (FC) PubMed
  11. Iannello A, et al. 2010. J. Immunol. 184:114. (FC) PubMed
  12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  13. Guereau-de-Arellan M, et al. 2011. Brain. 134:3578. PubMed
  14. Canque B, et al. 2000. Blood 96:3748. (ICC)
  15. Imanguli MM, et al. 2009. Blood 13:3620. (ICC)
  16. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  17. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Sckisel G, et al. 2017. J Immunother Cancer . 10.1186/s40425-017-0235-4. PubMed
  2. Balakrishnan A, et al. 2017. Biol Blood Marrow Transplant. 10.1016/j.bbmt.2017.07.016. PubMed
  3. Mukhopadhyay M, et al. 2017. J Immunol. 10.4049/jimmunol.1700953. PubMed
  4. Lichtenegger FS, et al. 2018. Front Immunol. 9:385. PubMed
  5. Carisey AF, et al. 2018. Curr Biol. 28:489. PubMed
  6. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  7. Claireaux M, et al. 2018. MBio. 9:e00317. PubMed
  8. Sagnella SM, et al. 2020. Cancer Cell. 37(3):354-370. PubMed
  9. Sekine T, et al. 2020. Cell. 183:158. PubMed
  10. Causi E, et al. 2015. PLoS One. 10: 0136717. PubMed
  11. Thommen D, et al. 2015. Cancer Immunol Res. 3: 1344 - 1355. PubMed
  12. Graav G, et al. 2016. PLoS One. 11: 0148604. PubMed
  13. Ward D, et al. 2016. Haematologica. 101: 286 - 296. PubMed
  14. Ryan J, et al. 2016. Proc Natl Acad Sci U S A. 113: 1286 - 1295. PubMed
  15. Penkava F, et al. 2020. Nat Commun. 3.76875. PubMed
  16. Ma X, et al. 2021. Cell Metabolism. 33(5):1001-1012.e5. PubMed
  17. Xu S, et al. 2021. Immunity. . PubMed
  18. Tarke A, et al. 2021. Cell Reports Medicine. 2(7):100355. PubMed
  19. Schouest B, et al. 2021. J Virol. 95:. PubMed
  20. Yankova E, et al. 2021. Nature. 593:597. PubMed
  21. Lu J, et al. 2021. Front Immunol. 12:732612. PubMed
  22. Mudd PA, et al. 2022. Cell. 185:603. PubMed
  23. Mo Y, et al. 2022. Front Immunol. 12:799896. PubMed
  24. Yu ED, et al. 2022. Cell Host Microbe. . PubMed
  25. Jarosch S, et al. 2022. STAR Protoc. 3:101374. PubMed
  26. Sehgal R, et al. 2022. Front Immunol. 13:828949. PubMed
  27. Celik H, et al. 2021. Cancer Discov. 11:3126. PubMed
  28. Peluso MJ, et al. 2021. Cell Rep. 36:109518. PubMed
  29. Yu ED, et al. 2022. Cell Host Microbe. 30:1269. PubMed
  30. Weidner T, et al. 2021. Nat Protoc. 16:3210. PubMed
  31. Reuschl AK, et al. 2022. Cell Rep. 39:110650. PubMed
  32. Jo T, et al. 2022. Cancer Immunol Immunother. Online ahead of print. PubMed
  33. Zhong W, et al. 2022. Front Immunol. 13:1001255. PubMed
  34. Gartshteyn Y, et al. 2023. Life Sci Alliance. 6: . PubMed
  35. Imai H, et al. 2023. iScience. 26:106822. PubMed
  36. Zhang C, et al. 2023. Front Immunol. 14:1182409. PubMed
RRID
AB_10900421 (BioLegend Cat. No. 304129)
AB_10965547 (BioLegend Cat. No. 304130)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane (exon 4 splicing of CD45 gene), 205-220 kD
Distribution

B cells, naïve T cells, monocytes

Function
Enhances TCR and BCR signaling
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.

Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45RA on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 2    Revision Date: 07.25.2013

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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