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Brilliant Violet 605™ anti-mouse CD62L Antibody

Pricing & Availability
Clone
MEL-14 (See other available formats)
Regulatory Status
RUO
Other Names
L-selectin, LECAM-1, Ly-22, LAM-1, MEL-14
Isotype
Rat IgG2a, κ
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Product Citations
publications
MEL-14_BV605_020312
C57BL/6 mouse splenocytes cells were stained with CD62L (clone MEL-14) Brilliant Violet 605™.
  • MEL-14_BV605_020312
    C57BL/6 mouse splenocytes cells were stained with CD62L (clone MEL-14) Brilliant Violet 605™.
See Brilliant Violet 605™ spectral data
Cat # Size Price Quantity Check Availability Save
104437 125 µL 248 CHF
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104438 50 µg 273 CHF
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Description

CD62L is a 74-95 kD glycoprotein also known as L-selectin, LECAM-1, Ly-22, LAM-1, and MEL-14. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte "rolling" on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naïve, effector, and memory T cells. CD62L has been reported to interact with CD34, GlyCAM-1, and MAdCAM-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/eb mouse B lymphoma 38C-13
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunofluorescent staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, complement-dependent cytotoxicity4, in vivo and in vitro blocking of adhesion1-3,5, and immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections6. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104457-104462).

Application References

(PubMed link indicates BioLegend citation)
  1. Gallatin WM, et al. 1983. Nature 304:30. (IP, Block)
  2. Siegelman MH, et al. 1990. Cell 61:611. (IP, Block)
  3. Lewinsohn DM, et al. 1987. J. Immunol. 138:4313. (IP, Block)
  4. Iwabuchi K, et al. 1991. Immunobiology 182:161. (CMCD)
  5. Pizcueta P, et al. 1994. Am. J. Pathol. 145:461.
  6. Reichert RA, et al. 1986. J. Immunol. 136:3535. (IHC, FC)
  7. Olver S, et al. 2006. Cancer Res. 66:571.
  8. Fukushima A, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:657. PubMed
  9. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Lee JW, et al. 2006. Nature Immunol. 8:181.
  12. Shigeta A, et al. 2008. Blood 112:4915 (FC) PubMed
  13. de Vries VC, et al. 2009. Am. J. Transplant. 9:2270 PubMed
Product Citations
  1. Cobbold SP, et al. 2018. Front Immunol. 9:1381. PubMed
  2. Wang D, et al. 2018. Immunity. 48:659. PubMed
  3. Waight JD, et al. 2018. Cancer Cell. 33:1033. PubMed
  4. Beura LK, et al. 2018. Immunity. 48:327. PubMed
  5. Cao W, et al. 2017. Immunity. 47:1182. PubMed
  6. Walker JA, et al. 2020. Immunity. 51(1):104-118. PubMed
  7. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  8. Gagnon JD, et al. 2019. Cell Rep. 28:2169. PubMed
  9. Chiou NT et al. 2018. Cell reports. 25(12):3356-3370 . PubMed
  10. Demircioglu F, et al. 2020. Nat Commun. 11:1290. PubMed
  11. Loo CS, et al. 2020. Immunity. 53:143. PubMed
  12. Andersen L, et al. 2020. Cell Reports. 29(13):4447-4459.e6.. PubMed
  13. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  14. Gutierrez D, et al. 2014. Diabetes. 63:3827. PubMed
  15. D’Amico L, et al. 2016. J Exp Med. 213: 827 - 840. PubMed
  16. Pérol L, et al. 2016. Nat Commun. 7:13027. PubMed
  17. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  18. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
  19. Dumas AA, et al. 2020. EMBO J. 39:e103790. PubMed
  20. Borges da Silva H, et al. 2020. Immunity. 53(1):158-171.e6. PubMed
  21. Weulersse M, et al. 2020. Immunity. 53(4):824-839.e10. PubMed
  22. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  23. Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed
  24. Barkaway A, et al. 2021. Immunity. . PubMed
  25. Dong L, et al. 2021. Cancer Cell. . PubMed
  26. Montalban-Arques A, et al. 2021. Cell Host Microbe. :. PubMed
  27. Korin B, et al. 2020. Sleep. :43. PubMed
  28. Yin Q, et al. 2021. Proc Natl Acad Sci U S A. 118: . PubMed
  29. Subramaniam N, et al. 2021. Blood Adv. 5:1259. PubMed
  30. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  31. Rao E, et al. 2021. Sci Immunol. 6:. PubMed
  32. Zebley CC, et al. 2021. Cell Rep. 37:109796. PubMed
  33. Pardy RD, et al. 2021. Nat Commun. 12:4051. PubMed
  34. Coe D, et al. 2022. JCI Insight. 7:. PubMed
  35. Kwok T, et al. 2022. Front Aging. 3:838943. PubMed
  36. Hu W, et al. 2021. Nat Immunol. 22:1163. PubMed
  37. Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
  38. Pierson M, et al. 2021. Curr Protoc. 1:e53. PubMed
  39. Peng C, et al. 2022. Immunity. 55:98. PubMed
  40. Liedmann S, et al. 2022. Mol Cell. 82:2401. PubMed
  41. Gonçalves R, et al. 2023. iScience. 26:105972. PubMed
  42. Dhalech AH, et al. 2022. J Virol. 96:e0123222. PubMed
  43. Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed
  44. Schroeter CB, et al. 2022. J Neuroinflammation. 19:270. PubMed
  45. Masle-Farquhar E, et al. 2023. Front Immunol. 14:1095257. PubMed
RRID
AB_11125577 (BioLegend Cat. No. 104437)
AB_2563058 (BioLegend Cat. No. 104438)

Antigen Details

Structure
Selectin, 95 kD (neutrophils) or 74 kD (lymphocytes)
Distribution

Subsets of B and T cells, monocytes, granulocytes, subset of NK cells

Function
Lymphocyte homing to HEV, rolling on activated endothelium
Ligand/Receptor
CD34, GlyCAM-1, MAdCAM-1
Cell Type
B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kishimoto TK, et al. 1990. P. Natl. Acad. Sci. USA 87:2244.
3. Tedder TF, et al. 1995. J. Exp. Med. 181:2259.

Gene ID
20343 View all products for this Gene ID
UniProt
View information about CD62L on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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