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Brilliant Violet 785™ anti-mouse/human CD45R/B220 Antibody

Pricing & Availability
Clone
RA3-6B2 (See other available formats)
Regulatory Status
RUO
Other Names
B220
Isotype
Rat IgG2a, κ
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Product Citations
publications
RA3-6B2_BV785_061812
C57BL/6 mouse splenocytes were stained with CD45R/B220 (clone RA3-6B2) Brilliant Violet 785™.
  • RA3-6B2_BV785_061812
    C57BL/6 mouse splenocytes were stained with CD45R/B220 (clone RA3-6B2) Brilliant Violet 785™.
See Brilliant Violet 785™ spectral data
Cat # Size Price Quantity Check Availability Save
103245 125 µL 209 CHF
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103246 50 µg 273 CHF
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Description

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse,Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunofluorescent staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.

Application References

(PubMed link indicates BioLegend citation)
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  14. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  15. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  2. Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
  3. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  4. Orr MT, et al. 2019. NPJ Vaccines. 4:1. PubMed
  5. Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
  6. Hartwig T et al. 2018. Cell reports. 25(13):3564-3572 . PubMed
  7. Mintz MA, et al. 2019. Immunity. 51:310. PubMed
  8. Tran NT, et al. 2019. Cell Rep. 28:3510. PubMed
  9. Mesin L, et al. 2020. Cell. 180(1):92-106.e11.. PubMed
  10. Blagih J, et al. 2020. Cell Rep. 30:481. PubMed
  11. Vanderleyden I, et al. 2020. Cell Rep. 30:611. PubMed
  12. Hollern DP, et al. 2020. Cell. 179(5):1191-1206.e21.. PubMed
  13. Pennock ND, et al. 2018. J Immunother Cancer. 0.318055556. PubMed
  14. Benhamou D, et al. 2018. Front Immunol. 2.260416667. PubMed
  15. Ochiai S, et al. 2014. J Immunol. 193:2504. PubMed
  16. Ahadome S, et al. 2016. JCI Insight. 1:e87012. PubMed
  17. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  18. Gallizioli M, et al. 2020. Cell Rep. 33:108291. PubMed
  19. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
  20. Delgado-Benito V, et al. 2020. J Exp Med. 217:00:00. PubMed
  21. Webb LMC, et al. 2021. Aging Cell. 20:e13295. PubMed
  22. Ruhland MK, et al. 2020. Cancer Cell. 37(6):786-799.e5. PubMed
  23. Silva-Cayetano A, et al. 2020. Med. 2(3):243-262.e8. PubMed
  24. Montalban-Arques A, et al. 2021. Cell Host Microbe. :. PubMed
  25. Nascimento DC, et al. 2021. Immunity. :. PubMed
  26. Verheijen M, et al. 2020. Cell Rep. 108376:33. PubMed
  27. Hofmann J, et al. 2021. Front Immunol. 11:599495. PubMed
  28. Onodera T, et al. 2021. Immunity. 54:2385. PubMed
  29. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  30. Kline JM, et al. 2021. J Biol Chem. :100373. PubMed
  31. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  32. Almutairi F, et al. 2021. Front Immunol. 12:772288. PubMed
  33. Chavez JS, et al. 2022. Cells. 11:. PubMed
  34. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  35. Bello A, et al. 2021. Immunol Cell Biol. 99:879. PubMed
  36. Duan L, et al. 2021. Immunity. 54:2256. PubMed
  37. Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
  38. Mujal AM, et al. 2022. Cancer Immunol Res. 10:403. PubMed
  39. Lee JL, et al. 2022. Aging Cell. 21:e13692. PubMed
  40. Lee JH, et al. 2022. Cell Rep. 38:110485. PubMed
  41. Halin C, et al. 2022. EMBO J. 41:e111528. PubMed
  42. Schiepers A, et al. 2023. Nature. 615:482. PubMed
  43. Lombard-Vadnais F, et al. 2023. iScience. 26:105852. PubMed
  44. Xiong E, et al. 2023. Front Immunol. 13:1083119. PubMed
  45. Yeh CH, et al. 2022. Immunity. 55:272. PubMed
  46. Kedmi R, et al. 2022. Nature. 610:737. PubMed
  47. Jia DJ, et al. 2022. Gut Microbes. 14:2145843. PubMed
  48. Pasqual G, et al. 2023. Methods Mol Biol. 2618:71. PubMed
RRID
AB_11218795 (BioLegend Cat. No. 103245)
AB_2563256 (BioLegend Cat. No. 103246)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about CD45R on UniProt.org
Go To Top Version: 2    Revision Date: 04.25.2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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