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FITC anti-human CD326 (EpCAM) Antibody

Pricing & Availability
Clone
9C4 (See other available formats)
Regulatory Status
RUO
Other Names
Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, TROP1
Isotype
Mouse IgG2b, κ
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Product Citations
publications
9C4_FITC_020708
Human colon carcinoma cell line HT29 stained with 9C4 FITC
  • 9C4_FITC_020708
    Human colon carcinoma cell line HT29 stained with 9C4 FITC
See FITC spectral data
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324203 25 tests 106 CHF
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324204 100 tests 235 CHF
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Description

CD326 is also known as Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, and TROP1. CD326 is a type I transmembrane protein containing six disulfide bridges and one THYRO domain. This cell surface glycosylated 40 kD protein is highly expressed in bone marrow, colon, lung, and most normal epithelial cells and is expressed on carcinomas of gastrointestinal origin. Recently, it has been reported that CD326 expression occurs during the early steps of erythrogenesis. CD326 functions as a homotypic calcium-independent cell adhesion molecule and is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation. CD326 antigen is an immunotherapeutic target for the treatment of human carcinomas.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
DU.4475 breast carcinoma
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the revelant formats) include: immunofluorescence, immunohistochemistry3, and spatial biology (IBEX)4,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Lammers R, et al. 2002. Exp. Hematol. 30:537.
  2. Schultz LD, et al. 2010. P. Natl. Acad. Sci. USA 107:13022. PubMed
  3. Human Protein Atlas http://www.proteinatlas.org/ENSG00000119888/antibody (IHC)
  4. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Kang H, et al. 2017. PLoS One. 12(6):e0180251. PubMed
  2. Zhao Q, et al. 2018. Cancer Res. 78:2370. PubMed
  3. Mair B et al. 2019. Nat Biomed Eng. 3(10):796-805 . PubMed
  4. McGinnis CS, et al. 2019. Nat Methods. 1.096527778. PubMed
  5. Xuan Z, et al. 2020. Am J Cancer Res. 0.458333333. PubMed
  6. Dinh PC, et al. 2020. Nat Commun. 1.197222222. PubMed
  7. Roca H, et al. 2013. PLoS One. 8:76773. PubMed
  8. Tanimizu N, et al. 2013. J Cell Sci. 126:5239. PubMed
  9. Jaganathan H, et al. 2014. PLoS One. 9:91986. PubMed
  10. Peng J, et al. 2015. Cancer Res . 75: 5034 - 5045. PubMed
  11. Barnicle A, et al. 2016. Physiol Genomics. 48: 257-273. PubMed
  12. Hao M, et al. 2021. Developmental Cell. 56(3):341-355.e5. PubMed
  13. Zheng X, et al. 2020. Cancer Res. 4414:80. PubMed
  14. Liu J, et al. 2020. Cell Mol Gastroenterol Hepatol. . PubMed
  15. Cassandras M, et al. 2020. Nat Cell Biol. 1295:22. PubMed
  16. Wimberger T, et al. 2020. Bioelectrochemistry. 134:107495. PubMed
  17. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
  18. Fasanya HO, et al. 2021. J Bone Oncol. 28:100357. PubMed
  19. Garcia TM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:943. PubMed
  20. Leonard NA, et al. 2021. Cancers (Basel). 13:. PubMed
  21. Martins Garcia T, et al. 2022. Antibiotics (Basel). 11:. PubMed
  22. Hansen FJ, et al. 2022. Int J Mol Sci. 23:. PubMed
  23. Jung Y, et al. 2020. Prostate. 80:1145. PubMed
  24. Choi J, et al. 2021. Nat Cell Biol. 23:953. PubMed
  25. Roos FJM, et al. 2021. Clin Transl Med. 11:e566. PubMed
  26. Dong L, et al. 2020. BJU Int. 126:191. PubMed
  27. Urbaniak A, et al. 2020. J Biochem Mol Toxicol. 34:e22487. PubMed
  28. Wu SZ, et al. 2020. EMBO J. 39:e104063. PubMed
  29. Liang D, et al. 2022. Front Cell Dev Biol. 10:883314. PubMed
  30. Jowett GM, et al. 2022. Cell Rep. 40:111281. PubMed
  31. Lütge M, et al. 2023. Nat Immunol. . PubMed
  32. Vokshi BH, et al. 2023. Nat Commun. 14:3034. PubMed
  33. Chen Z, et al. 2023. Nat Commun. 14:3469. PubMed
RRID
AB_756077 (BioLegend Cat. No. 324203)
AB_756078 (BioLegend Cat. No. 324204)

Antigen Details

Structure
Type I transmembrane protein, contains six disulfide bridges, one THYRO domain, approximate molecular weight 40 kD.
Distribution

Highly expressed in bone marrow, colon, lung, and most normal epithelial cells. Also highly expressed on carcinomas of gastrointestinal origin. Expressed during early erythrogenesis.

Function
Homotypic calcium-independent cell adhesion. CD326 is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation.
Modification
Glycosylated.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Strnad J, et al. 1989. Cancer Res. 49:314.
2. Munz M, et al. 2004. Oncogene 23:5748.
3. Rao CG, et al. 2005. Int. J. Oncol. 27:49.

Gene ID
4072 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

Related FAQs

There are no FAQs for this product.
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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