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Pacific Blue™ anti-human CD3 Antibody

Pricing & Availability
Clone
OKT3 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T3, CD3ε
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
OKT3_PB_CD3_Antibody_FC_031213
Human peripheral blood lymphocytes were stained with CD3 (clone OKT3) Pacific Blue™ (filled histogram) or mouse IgG2a, κ Pacific Blue™ isotype control (open histogram).
  • OKT3_PB_CD3_Antibody_FC_031213
    Human peripheral blood lymphocytes were stained with CD3 (clone OKT3) Pacific Blue™ (filled histogram) or mouse IgG2a, κ Pacific Blue™ isotype control (open histogram).
See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability Save
317313 25 µg 124 CHF
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317314 100 µg 265 CHF
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Description

CD3ε is a 20 kD chain of the CD3/T cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.

Clone OKT3 can block the binding of clones SK7 and UCHT1.4 The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  4. Li B, et al. 2005. Immunology 116:487.
  5. Jeong HY, et al. 2008. J. Leuckocyte Biol. 83:755. PubMed
  6. Alter G, et al. 2008. J. Virol. 82:9668. PubMed
  7. Manevich-Mendelson E, et al. 2009. Blood 114:2344. PubMed
  8. Pinto JP, et al. 2010. Immunology. 130:217. PubMed
  9. Biggs MJ, et al. 2011. J. R. Soc. Interface. 8:1462. PubMed
Product Citations
  1. Zaikos TD, et al. 2018. Cell Rep. 25:3759. PubMed
  2. Daniels J, et al. 2020. Nat Commun. 11:1806. PubMed
  3. Hope C, et al. 2014. Kidney Int. 86:175. PubMed
  4. Collins D, et al. 2015. PLoS Pathog. 11: 1005054. PubMed
  5. Guo X, et al. 2016. Mol Ther Methods Clin Dev. 3:15054. PubMed
  6. Bernardes JP, et al. 2020. Immunity. 53:1296. PubMed
  7. Qian Y, et al. 2021. Cell Reports. 36(8):109602. PubMed
  8. Charles TP, et al. 2021. PLoS Pathog. 17:e1009257. PubMed
  9. Welbourn S, et al. 2022. PLoS Pathog. 18:e1010488. PubMed
  10. Xia Q, et al. 2022. Methods Mol Biol. 2574:251. PubMed
  11. LaSalle TJ, et al. 2022. Cell Rep Med. 3:100779. PubMed
  12. Côrte-Real BF, et al. 2022. Front Immunol. 13:1005965. PubMed
  13. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
  14. Beck C, et al. 2023. Biomedicines. 11:. PubMed
RRID
AB_571908 (BioLegend Cat. No. 317313)
AB_571909 (BioLegend Cat. No. 317314)

Antigen Details

Structure
Ig superfamily, the subunits CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) form the CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
  2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
  3. Lanier L, et al. 1986. J. Immunol. 137:2501.
Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org
Go To Top Version: 3    Revision Date: 06.12.2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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