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Pacific Blue™ anti-mouse F4/80 Antibody

Pricing & Availability
Clone
BM8 (See other available formats)
Regulatory Status
RUO
Other Names
EMR1, Ly71
Isotype
Rat IgG2a, κ
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Product Citations
publications
BM8_Blue_021808
Thioglycollate elicited Balb/c peritoneal macrophage cells stained with Pacific Blue™ BM8
  • BM8_Blue_021808
    Thioglycollate elicited Balb/c peritoneal macrophage cells stained with Pacific Blue™ BM8
See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability Save
123123 25 µg 138 CHF
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123124 100 µg 303 CHF
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Description

F4/80, also known as EMR1 or Ly71, is a 160 kD glycoprotein of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80 has been widely used as a murine macrophage marker. It is expressed on a majority of tissue macrophages, including macrophages in the lung, gut, peritoneal cavity, thymus, and red pulp of the spleen, Kupffer cells, Langerhans cells, microglia, and certain dendritic cells. It is not expressed on macrophages located in the T cell areas of the spleen, lymph node, or Peyer's patch. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for the induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.

Application References

(PubMed link indicates BioLegend citation)
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Draijer C, et al. 2018. Sci Rep. 8:5105. PubMed
  2. Billi AC, et al. 2019. JCI Insight. 4:8. PubMed
  3. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  4. Schneider C, et al. 2018. Cell. 174:271. PubMed
  5. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  6. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  7. Ganeshan K et al. 2019. Cell. 177(2):399-413 . PubMed
  8. Leonard JD et al. 2017. Immunity. 47(1):107-117 . PubMed
  9. Kälin S et al. 2017. Cell metabolism. 26(3):475-492 . PubMed
  10. Murphy M, et al. 2017. Eur J Immunol. 47:880. PubMed
  11. Fotakis P, et al. 2019. Arterioscler Thromb Vasc Biol. 39:e253. PubMed
  12. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  13. Challa TD, et al. 2020. Cell Reports. 30(10):3424-3433. PubMed
  14. Jones M, et al. 2010. J Immunol. 185:3583. PubMed
  15. Goncalves R, et al. 2011. J Exp Med. 208:1253. PubMed
  16. Huanga J, et al. 2013. J Immunol Methods. 387:254. PubMed
  17. Ni P, et al. 2014. J Immunol . 193:1778. PubMed
  18. Stack G, et al. 2015. PLoS Pathog. 11:1004641. PubMed
  19. Syed A, et al. 2015. Infect Immun . 83: 3428-3437. PubMed
  20. Tanaka Y, et al. 2015. PLoS One. 10: 0138621. PubMed
  21. Krishnamurthy B, et al. 2015. Diabetes. 64: 3229-3238. PubMed
  22. Bucher K, et al. 2015. J Leukoc Biol. 2015;98: 365-372. PubMed
  23. Izquierdo-Useros N, et al. 2014. J Immunol . 192:4852-4858. PubMed
  24. Cohen H, et al. 2015. J Immunol. 195: 3828 - 3837. PubMed
  25. Saravia J, et al. 2015. PLoS Pathog. 11: e1005217. PubMed
  26. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  27. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  28. Kwon O, et al. 2016. Stem Cell Res. 16: 682-691. PubMed
  29. Stolt C, et al. 2016. J Immunol. 197: 834 - 846. PubMed
  30. Zhang B, et al. 2016. Mol Cell. 63: 976-89. PubMed
  31. Philip N, et al. 2016. PLoS Pathog. 12:e1005910. PubMed
  32. Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
  33. Zhang X, et al. 2020. Endocr Relat Cancer. 27:469. PubMed
  34. Flores AM, et al. 2020. Nat Nanotechnol. 0.731944444. PubMed
  35. Jard T, et al. 2020. Cell Stem Cell. 27(4):646-662.e7. PubMed
  36. Jiang L, et al. 2020. Cell. 183(5):1219-1233.e18. PubMed
  37. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  38. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  39. Marangoni F, et al. 2021. Cell. . PubMed
  40. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  41. Dichtl S, et al. 2021. Sci Adv. 7: . PubMed
  42. Yang Y, et al. 2021. Nat Commun. 12:525. PubMed
  43. Shah D, et al. 2021. Oncoimmunology. 10:1939601. PubMed
  44. Okamura T, et al. 2021. Front Immunol. 12:669629. PubMed
  45. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  46. Jee JJ, et al. 2022. Nat Commun. 13:18. PubMed
  47. Dar HH, et al. 2022. JCI Insight. 7:. PubMed
  48. Chen WS, et al. 2021. Cell Rep. 37:109974. PubMed
  49. Boukelmoune N, et al. 2021. Brain Behav Immun. 93:43. PubMed
  50. O'Leary CE, et al. 2021. Curr Protoc. 1:e77. PubMed
  51. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
  52. Gerlach BD, et al. 2021. Cell Metab. 33:2445. PubMed
  53. Xin B, et al. 2022. Hepatology. 76:630. PubMed
  54. Zhou Y, et al. 2022. Br J Pharmacol. 179:1978. PubMed
  55. Du Y, et al. 2022. FASEB J. 36:e22248. PubMed
  56. Shi C, et al. 2023. Nat Nanotechnol. 18:86. PubMed
RRID
AB_893487 (BioLegend Cat. No. 123123)
AB_893475 (BioLegend Cat. No. 123124)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kupffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org
Go To Top Version: 2    Revision Date: 10.25.2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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