- Clone
- HIM6 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- VI N-L109
- Other Names
- Neurothelin, Basigin, EMMPRIN
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![HIM6_PE_CD147_Antibody_072718 HIM6_PE_CD147_Antibody_072718](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/HIM6_PE_CD147_Antibody_072718.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Human peripheral blood lymphocytes were stained with CD147 (clone HIM6) PE (filled histogram) or Mouse IgG1, κ PE isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
306211 | 25 tests | 117 CHF | ||||
306212 | 100 tests | 262 CHF |
CD147, also known as neurothelin or basigin, is a member of the Ig superfamily. It is a 55-65 kD type I transmembrane glycoprotein which is primarily expressed on leukocytes, erythrocytes, platelets, and endothelial cells. CD147 is reported to have a function during embryonal brain development and/or play a role in integrin-mediated adhesion in brain endothelia.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human PBMCs
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: inhibition of T cell activation2, immunohistochemical staining1,3 of frozen tissue sections and formalin-fixed paraffin-embedded tissue sections, and Western blotting1. The Ultra-LEAF™ Purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 306221 and 306222).
-
Application References
(PubMed link indicates BioLegend citation) -
- Menashi S, et al. 2003. Cancer Res. 63:7575. (WB IHC)
- Woodhead VE, et al. 2000. Int. Immunol. 12:1051. (Block)
- Reimers N, et al. 2004. Clin. Cancer Res. 10:3422. (IHC)
- Product Citations
- RRID
-
AB_2750167 (BioLegend Cat. No. 306211)
AB_2750168 (BioLegend Cat. No. 306212)
Antigen Details
- Structure
- Ig superfamily, type I transmembrane glycoprotein, 55-65 kD
- Distribution
-
Leukocytes, erythrocytes, platelets, endothelial cells
- Function
- Adhesion
- Cell Type
- Endothelial cells, Erythrocytes, Leukocytes, Platelets
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Biswas C, et al. 1995. Cancer Res. 55:434.
2. Fadool J, et al. 1993. Dev. Dyn. 196:252.
3. Felzmann T, et al. 1991. J. Clin. Immunol. 11:205. - Gene ID
- 682 View all products for this Gene ID
- UniProt
- View information about CD147 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Customers Also Purchased
![](https://d1spbj2x7qk4bg.cloudfront.net/Files//Images/media_assets/products/product_images/TRA-2-10_PE_061011.jpg)
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Follow Us