Loading...

PE anti-human CD3 Antibody

Pricing & Availability
Clone
UCHT1 (See other available formats)
Regulatory Status
RUO
Workshop
III 471
Other Names
T3, CD3ε
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
UCHT1
Human peripheral blood lymphocytes stained with UCHT1 PE
  • UCHT1
    Human peripheral blood lymphocytes stained with UCHT1 PE
  • UCHT1_PE_CD3_Antibody_042922
    Pre-lysed human blood leukocytes were stained with PE anti-human CD3 (clone UCHT1) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).

    Data was acquired on the Moxi Flow, exported, and processed using FlowJo software.
See PE spectral data
Cat # Size Price Quantity Check Availability Save
300407 25 tests 28 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
300408 100 tests 99 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
300456 100 µg 104 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
300441 500 tests 303 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Shoda H, et al. 2017. Arthritis Research & Therapy . 10.1186/s13075-017-1308-y. PubMed
  2. Yuan Z, et al. 2018. Emerg Microbes Infect. 7:59. PubMed
  3. Tiwarekar V, et al. 2018. J Virol. 92:17. PubMed
  4. Miggitsch C, et al. 2019. EBioMedicine. 46:387. PubMed
  5. Wang X, et al. 2020. Exp Ther Med. 19:3698. PubMed
  6. Teijeira á, et al. 2020. Immunity. 52(5):856-871. PubMed
  7. Sun B, et al. 2019. Cancer Immunol Res. 1.550694444. PubMed
  8. Thakral D, et al. 2008. J Immunol. 180:7431. PubMed
  9. Tomita K, et al. 2013. Neuroscience. 236:55. PubMed
  10. Wang L, et al. 2014. Proc Natl Acad Sci U S A. 111:3146. PubMed
  11. Groen R, et al. 2015. J Leukoc Biol. 98: 623 - 630. PubMed
  12. O’Connor K, et al. 2016. Genes Immun. 10.1038/gene.2016.27. PubMed
  13. Yuan Z, et al. 2016. J Virol. 90: 7728 - 7739. PubMed
  14. Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed
  15. Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
  16. Mineo M, et al. 2020. Molecular Cell. 78(6):1207-1223.e8. PubMed
  17. Nabet BY, et al. 2020. Cell. 183(2):363-376.e13. PubMed
  18. Bonifacius A, et al. 2021. Immunity. 54(2):340-354.e6. PubMed
  19. Lanz AL, et al. 2021. Cell Reports. 36(2):109375. PubMed
  20. Dong G, et al. 2020. Haematologica. . PubMed
  21. Deng W, et al. 2021. Emerg Microbes Infect. 10:1272. PubMed
  22. Singh KS, et al. 2021. Nature. 589:597. PubMed
  23. Hirama T, et al. 2021. JCI Insight. 6:. PubMed
  24. Ramendra R, et al. 2021. Front Immunol. 12:694152. PubMed
  25. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
  26. Georg P, et al. 2022. Cell. 185:493. PubMed
  27. Bessy T, et al. 2021. J Cell Biol. 220:. PubMed
  28. Zhao NQ, et al. 2020. PLoS One. 15:e0238347. PubMed
  29. Mysore V, et al. 2021. Med (N Y). 2:1050. PubMed
  30. Yu W, et al. 2022. Front Cell Dev Biol. 10:928140. PubMed
  31. Merah-Mourah F, et al. 2021. Curr Protoc. 1:e229. PubMed
  32. Hsiao CC, et al. 2022. STAR Protoc. 3:101422. PubMed
  33. Jeon SB, et al. 2022. Int J Stem Cells. Online ahead of print. PubMed
  34. Sefik E, et al. 2022. Nature. 606:585. PubMed
  35. Sušac L, et al. 2022. Cell. 185:3201. PubMed
  36. Jenkins E, et al. 2023. Nat Commun. 14:1611. PubMed
RRID
AB_314061 (BioLegend Cat. No. 300407)
AB_314062 (BioLegend Cat. No. 300408)
AB_2564150 (BioLegend Cat. No. 300456)
AB_2562047 (BioLegend Cat. No. 300441)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Customers Also Purchased

APC anti-human CD3
Clone: UCHT1

FITC anti-human CD3
Clone: UCHT1

PE anti-human CD19
Clone: HIB19

PE anti-human CD14
Clone: M5E2

Go To Top Version: 1    Revision Date: 11.30.2012

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account