Loading...

PE/Cyanine7 anti-human CD8 Antibody

Pricing & Availability
Clone
SK1 (See other available formats)
Regulatory Status
RUO
Other Names
T8, Leu2
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
SK1_PECy7_021610
Human peripheral blood lymphocytes stained with SK1 PE/Cyanine7
  • SK1_PECy7_021610
    Human peripheral blood lymphocytes stained with SK1 PE/Cyanine7
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
344711 25 tests 138 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
344750 100 µg 229 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
344712 100 tests 325 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
µg size: 0.2 mg/mL
Test sizes: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics8, immunohistochemistry of acetone-fixed frozen tissue sections, and spatial biology (IBEX)9,10. This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter JA, et al. 1981. J. Exp. Med. 153:310.
  2. Campanelli R, et al. 2002. Intl. Immunol. 14:39.
  3. Evans RL, et al. 1981. Immunol. 78:544.
  4. Wooldridge L, et al. 2005. J. Bio. Chem. 280:27491.
  5. Ch'el IL, et al. 2011. J Exp Med. 208:633. PubMed
  6. Carbone A, et al. 1999. Blood 93:2319. (IHC-F)
  7. Ahmed A, et al. 2001. J. Pathol. 193:383. (IHC)
  8. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  9. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  10. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Buchan SL et al. 2018. Immunity. 49(5):958-970 . PubMed
  2. Neal JT, et al. 2018. Cell. 175:1972. PubMed
  3. Franco LM, et al. 2019. J Exp Med. 216:384. PubMed
  4. Renner K, et al. 2020. Cell Reports. 29(1):135-150.e9.. PubMed
  5. Arif S, et al. 2020. Diabetologia. 63(6):1186-1198.. PubMed
  6. Lickliter JD, et al. 2020. Drug Des Devel Ther. 14:1177. PubMed
  7. van Beek JJP, et al. 2020. Cell Reports. 30(4):1027-1038.e4.. PubMed
  8. Lima J, et al. 2016. Reprod Sci. 10.1177/1933719116653680. PubMed
  9. Iannetta M, et al. 2016. PLoS One. 11: 0160277. PubMed
  10. James KR, et al. 2020. Nat Immunol. 1.113194444. PubMed
  11. Pierce CA, et al. 2020. Sci Transl Med. 12:00. PubMed
  12. Bonifacius A, et al. 2021. Immunity. 54(2):340-354.e6. PubMed
  13. Chen Y, et al. 2020. Cell. 1496:183. PubMed
  14. Alsaleh G, et al. 2020. Elife. 9: . PubMed
  15. Liu R, et al. 2021. Commun Biol. 4:652. PubMed
  16. Ho CH, et al. 2021. Arthritis Res Ther. 23:199. PubMed
  17. Hohmann MS, et al. 2021. JCI Insight. 6:. PubMed
  18. Ma C, et al. 2021. Signal Transduct Target Ther. 6:353. PubMed
  19. Manukyan G, et al. 2020. Auto Immun Highlights. 11:5. PubMed
  20. Arenas EJ, et al. 2021. Nat Commun. 12:1237. PubMed
  21. Gussarow D, et al. 2021. Front Med (Lausanne). 8:770381. PubMed
  22. Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed
  23. Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
  24. Mo Y, et al. 2022. Front Immunol. 12:799896. PubMed
  25. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  26. Dai G, et al. 2021. JCI Insight. 6:. PubMed
  27. Hu J, et al. 2022. J Immunother Cancer. 10:. PubMed
  28. Lai Y, et al. 2022. Clin Transl Med. 12:e999. PubMed
  29. Batikan O, et al. 2022. J Coll Physicians Surg Pak. 32:1004. PubMed
  30. Wang H, et al. 2022. iScience. 25:105065. PubMed
  31. Castellano E, et al. 2022. Front Immunol. 13:970931. PubMed
  32. Song M, et al. 2022. J Gene Med. 24:e3455. PubMed
  33. Reitinger C, et al. 2022. Front Immunol. 13:970290. PubMed
  34. Martínez-Sabadell A, et al. 2022. STAR Protoc. 3:101712. PubMed
  35. Bhatt RS, et al. 2021. Cancer Immunol Res. 9:156. PubMed
  36. Yuan H, et al. 2022. J Clin Exp Hematop. 62:52. PubMed
  37. Stunnenberg M, et al. 2022. J Leukoc Biol. 112:289. PubMed
  38. Zhang T, et al. 2022. Am J Reprod Immunol. 87:e13524. PubMed
  39. Chen Y, et al. 2022. Sci Immunol. 7:eabp8328. PubMed
  40. Idiz UO, et al. 2022. J Med Virol. 94:4744. PubMed
  41. Sušac L, et al. 2022. Cell. 185:3201. PubMed
  42. Martínez-Sabadell A, et al. 2022. Cell Rep. 41:111430. PubMed
  43. Ritacco C, et al. 2023. iScience. 26:106085. PubMed
  44. Battistello E, et al. 2023. Mol Cell. 83:1216. PubMed
  45. Saeland E, et al. 2023. NPJ Vaccines. 8:45. PubMed
  46. van Os BW, et al. 2023. Eur Heart J Open. 3:oead013. PubMed
  47. Boucher LE, et al. 2023. MAbs. 15:2195517. PubMed
  48. Xiao Q, et al. 2023. Bio Protoc. 13:e4655. PubMed
  49. Imai H, et al. 2023. iScience. 26:106822. PubMed
RRID
AB_2044007 (BioLegend Cat. No. 344711)
AB_2687201 (BioLegend Cat. No. 344750)
AB_2044008 (BioLegend Cat. No. 344712)

Antigen Details

Structure
Ig superfamily, homodimer or heterodimer with CD8b, 32-34 kD
Distribution

Majority of thymocytes, T cell subset, NK cells

Function
MHC class I co-receptor, thymic differentiation, T cell activation
Ligand/Receptor
MHC Class I molecules
Cell Type
NK cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.

Gene ID
925 View all products for this Gene ID
UniProt
View information about CD8 on UniProt.org
Go To Top Version: 4    Revision Date: 07.13.2015

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account