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PE/Dazzle™ 594 anti-human CD3 Antibody

Pricing & Availability
Clone
SK7 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T3, CD3ε
Isotype
Mouse IgG1, κ
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Product Citations
publications
SK7_PEDZL594_CD3_Antibody_061016
Human peripheral blood lymphocytes were stained with CD3 (clone SK7) PE/Dazzle™ 594 (filled histogram) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (open histogram).
  • SK7_PEDZL594_CD3_Antibody_061016
    Human peripheral blood lymphocytes were stained with CD3 (clone SK7) PE/Dazzle™ 594 (filled histogram) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (open histogram).
See PE/Dazzle™ 594 spectral data
Cat # Size Price Quantity Check Availability Save
344843 25 tests 159 CHF
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344844 100 tests 393 CHF
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections4,5,8, immunofluorescent staining6, and Western blotting3.

Application References

(PubMed link indicates BioLegend citation)
  1. Kan EA, et al. 1983. J. Immunol. 131:536.
  2. Wood GS, et al. 1985. Am. J. Pathol. 120:371.
  3. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  4. Haringman JJ, et al. 2005. Arthritis Res. Ther. 7:R862. (IHC)
  5. Carbone A, et al. 1999. Blood 93:2319. (IHC)
  6. Goval JJ, et al. 2006. J. Histochem. Cytochem. 54:75. (IF)
  7. Rutjens E, et al. 2007. J. Immunol. 178:1702.
  8. Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Product Citations
  1. Shishkova D, et al. 2022. Int J Mol Sci. 23: . PubMed
RRID
AB_2616969 (BioLegend Cat. No. 344843)
AB_2616970 (BioLegend Cat. No. 344844)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ, (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, during thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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