- Clone
- QA16A02 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-aseGranzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-ase
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![QA16A02_PEDazzle594_GranzymeB_Antibody_032118 QA16A02_PEDazzle594_GranzymeB_Antibody_032118](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/QA16A02_PEDazzle594_GranzymeB_Antibody_032118.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood mononuclear cells were stained with CD8 FITC, fixed, permeabilized, and then stained with Granzyme B (clone QA16A02, left) PE/Dazzle™ 594 or mouse IgG1, κ PE/Dazzle™ 594 isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
372215 | 25 tests | 159 CHF | ||||
372216 | 100 tests | 370 CHF |
Granzyme B is a 32 kD serine protease, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.
Product DetailsProduct Details
- Reactivity
- Human,Mouse
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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ICFC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Product Citations
- RRID
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AB_2728382 (BioLegend Cat. No. 372215)
AB_2728383 (BioLegend Cat. No. 372216)
Antigen Details
- Structure
- 32 kD serine protease
- Distribution
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Cytotoxic T cells, NK cells, and neutrophils, low on granulocytes, B cells and activated dendritic cells
- Function
- Granzyme B is able to induce target cell apoptosis by activating caspase independent pathways. Granzyme B is induced in CD8+ T lymphocytes with ConA/ IL-2 and CD4+ T lymphocytes with anti CD3/CD28 or CD3/CD46.
- Interaction
- Caspase-3
- Ligand/Receptor
- Mannose-6-phosphate receptor
- Cell Type
- T cells, NK cells, Neutrophils
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience
- Molecular Family
- Proteases, Enzymes and Regulators
- Antigen References
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1. Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203.
2. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885.
3. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610.
4. Wagner C, et al. 2008. Mol. Immunol. 45:1761. - Gene ID
- 3002 View all products for this Gene ID 14939 View all products for this Gene ID
- UniProt
- View information about Granzyme B on UniProt.org
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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