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Purified anti-DYKDDDDK Tag Antibody

Pricing & Availability
Clone
L5 (See other available formats)
Regulatory Status
RUO
Other Names
FLAG tag
Isotype
Rat IgG2a, λ
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Product Citations
publications
L5_WB_100608
Cell extracts from untransfected 293T cells (lane 1) or 293T cells transfected with a plasmid encoding DYKDDDDK-tagged protein (lane 2), using anti-DYKDDDDK, clone L5.
  • L5_WB_100608
    Cell extracts from untransfected 293T cells (lane 1) or 293T cells transfected with a plasmid encoding DYKDDDDK-tagged protein (lane 2), using anti-DYKDDDDK, clone L5.
Cat # Size Price Quantity Check Availability Save
637319 25 µg 89 CHF
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637301 200 µg 253 CHF
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637302 500 µg 382 CHF
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637303 1 mg 475 CHF
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637304 5 mg 780 CHF
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Description

The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins.This unique amino acid sequence allows for specific antibody detection in western blotting, immunoprecipitation, and immunostaining techniques.Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Epitope tag
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
DYKDDDDK-tagged mouse Langerin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP, ICC, ELISA, FC, Purification - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The L5 clone has been demonstrated to have 2-8 fold better sensitivity in WB than another commonly used antibody clone, M2.

Application References

(PubMed link indicates BioLegend citation)
  1. Park SH, et al. 2008. J Immunol Methods. 331:27.
  2. Moon SH, et al. 2010. J. Biol Chem. 285:12935. PubMed
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  4. Sonder SU, et al. 2012. J Immunol. 188:5906. PubMed
  5. Jiang Y, et al. 2013. Int Immunol. 25:235. PubMed
  6. Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
  7. Toyo-Oak K, et al. 2014. J Neurosci. 34:12168. PubMed
Product Citations
  1. Mamedov T, et al. 2017. PLoS One.. 10.1371/journal.pone.0183589. PubMed
  2. Bala Tannan N, et al. 2018. PLoS Genet. 14:e1007153. PubMed
  3. Wroblewska A et al. 2018. Cell. 175(4):1141-1155 . PubMed
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  6. Wen XY, et al. 2018. JCI Insight. 3:. PubMed
  7. Mamedov T, et al. 2019. PLoS One. 14:e0213438. PubMed
  8. Stroukov W, et al. 2019. Front Genet. 10:163. PubMed
  9. Mamedov T, et al. 2019. Sci Rep. 9:9868. PubMed
  10. Xu C, et al. 2019. J Immunol. 203:2141. PubMed
  11. Liu X, et al. 2019. Nat Commun. 10:3020. PubMed
  12. Sundaram A et al. 2017. eLife. 6 pii: e27187. PubMed
  13. Golec E, et al. 2019. FASEB J. 33:12425. PubMed
  14. Willcox CR, et al. 2020. Immunity. 51(5):813-825.e4.. PubMed
  15. Karunakaran MM, et al. 2020. Immunity. 52:487. PubMed
  16. Yuan Y, et al. 2020. Nat Commun. 11:1472. PubMed
  17. Zhang S, et al. 2020. Nat Commun. 11:1312. PubMed
  18. Carlevaro G, et al. 2019. Methods Mol Biol. 1955:135. PubMed
  19. Cross SH, et al. 2020. PLoS Genet. 16:e1008583. PubMed
  20. Takahashi K, et al. 2020. Cell Reports. 31(9):107715. PubMed
  21. Moon S, et al. 2010. J Biol Chem. 285:12935. PubMed
  22. Sønder S, et al. 2012. J Immunol. 188:5906. PubMed
  23. Jiang Y, et al. 2013. Int Immunol. 25:235. PubMed
  24. Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
  25. Toyo-oka K, et al. 2014. J Neurosci. 34:12168. PubMed
  26. Yin J, et al. 2015. Brain. 138: 2553-2570. PubMed
  27. Ren S, et al. 2016. PLoS One. 11: 0152721. PubMed
  28. Das A, et al. 2016. J Biol Chem. 291: 6096 - 6110. PubMed
  29. Shi Y, et al. 2016. Cell Death Differ. 10.1038/cdd.2016.110. PubMed
  30. Bonet-Ponce L, et al. 2020. Sci Adv. 6:00. PubMed
  31. Yang Q, et al. 2020. Elife. 9:00. PubMed
  32. Zhu C, et al. 2020. Cell Rep. 31:107745. PubMed
  33. Yang M, et al. 2020. Appl Environ Microbiol. 86:00:00. PubMed
  34. Torres-Ayuso P, et al. 2021. Cancer Discovery. 11(6):1411-1423. PubMed
  35. Park GY, et al. 2020. Immune Netw. e43:20. PubMed
  36. Liu X, et al. 2021. Hum Mol Genet. 30:30. PubMed
  37. Asano Y, et al. 2021. Nat Commun. 12:4372. PubMed
  38. Fujii T, et al. 2021. Bone Res. 9:4. PubMed
  39. Kim J, et al. 2021. J Cell Biol. 220:. PubMed
  40. Driskill JH, et al. 2021. Genes Dev. 35:495. PubMed
  41. Sims S, et al. 2021. Adv Virol. 2021:5569844. PubMed
  42. Okamoto Y, et al. 2021. J Cell Sci. 134:. PubMed
  43. He H, et al. 2021. J Immunother Cancer. 9:. PubMed
  44. Figueroa F, et al. 2021. Front Cell Dev Biol. 9:739445. PubMed
  45. Sharma VP, et al. 2021. Nat Commun. 12:7300. PubMed
  46. Chaouat AE, et al. 2021. PLoS Pathog. 17:e1010175. PubMed
  47. Wu G, et al. 2021. Nat Cancer. 2:1170. PubMed
  48. Hu J, et al. 2021. Cell Death Dis. 12:391. PubMed
  49. Sandhu R, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  50. Nabar NR, et al. 2022. Autophagy. 18:204. PubMed
  51. Adam S, et al. 2021. Nat Cancer. 2:1357. PubMed
  52. Velay F, et al. 2022. Plant Methods. 18:69. PubMed
  53. Zang RX, et al. 2022. ACS Omega. 7:34378. PubMed
  54. Sánchez-Bellver L, et al. 2022. Int J Mol Sci. 23:. PubMed
  55. Trothen SM, et al. 2022. FEBS Lett. 596:232. PubMed
  56. Sun X, et al. 2022. Appl Environ Microbiol. 88:e0230321. PubMed
  57. Basavarajappa SC, et al. 2022. iScience. 25:104716. PubMed
  58. Wang W, et al. 2022. Cell Rep. 41:111582. PubMed
  59. Meleppattu S, et al. 2022. Cell. 185:4986. PubMed
  60. Setiaputra D, et al. 2022. Mol Cell. 82:1359. PubMed
  61. Kluss JH, et al. 2022. Neurobiol Dis. 170:105769. PubMed
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RRID
AB_2749907 (BioLegend Cat. No. 637319)
AB_1134266 (BioLegend Cat. No. 637301)
AB_1134268 (BioLegend Cat. No. 637302)
AB_1134265 (BioLegend Cat. No. 637303)
AB_1134267 (BioLegend Cat. No. 637304)

Antigen Details

Biology Area
Cell Biology
Antigen References

1. Einhauer A. 2001. J. Biochem. Biophys. Methods. 49:455.
2. Knappik A and Pluckthun A. 1994. Biotechniques. 17:754.

Gene ID
NA
UniProt
View information about DYKDDDDK on UniProt.org

Related FAQs

I am unable to detect FLAG tag with L5 antibody clone by IP and/or western blotting.
Some points to consider here include
  • Sometimes changing tag location (form N-terminus to C-terminus or vice versa) or use tandem FLAG tag (3x) can also increase the chances of tag detection
  • Tagged protein is either not expressed or lowly expressed
  • Always include a positive control to make sure the process of working fine
Does anti tag antibody recognize the tag sequence at N terminal or C terminal or within the fusion protein?
Our antibody can recognize the fusion protein with tag either at N-terminus or C-terminus or within the protein if the tag is exposed.
Go To Top Version: 4    Revision Date: 09.21.2023

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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