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Purified anti-human CD29 Antibody

Pricing & Availability
Clone
TS2/16 (See other available formats)
Regulatory Status
RUO
Workshop
V A-S202
Other Names
Integrin β1 chain, VLA-β chain, gpIIa, ITGB1
Isotype
Mouse IgG1, κ
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Product Citations
publications
TS216_Purified_062107
Human peripheral blood lymphocytes stained with purified TS2/16, followed by anti-mouse IgGs FITC
  • TS216_Purified_062107
    Human peripheral blood lymphocytes stained with purified TS2/16, followed by anti-mouse IgGs FITC
  • zTS2slash16_IF_072512
    BT474 breast cancer cells were stained with anti-CD29 (clone TS2/16) followed by DyLight™ 649 Goat anti-mouse Ig secondary antibody (red), plus DAPI staining for nuclei (blue). Images were taken under 20x bin4 (Filter set: EX647/10x, Dichroic 665LP, EM 700/70x) at exposure 4s. Data provided by Er Liu and John Nolan, La Jolla Institute for Bioengineering.
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303001 25 µg 59 CHF
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303002 100 µg 112 CHF
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Description

CD29 is a 130 kD single chain type I glycoprotein also known as integrin β1, VLA-β chain, or gpIIa. It is broadly expressed on a majority of hematopoietic and non-hematopoietic cells, including leukocytes (although at low level on granulocytes), platelets, fibroblasts, endothelial cells, epithelial cells, and mast cells. CD29 is a member of the integrin family. It is non-covalently associated with integrin α1-α6 chains to form VLA-1 to VLA-6 molecules, respectively. Integrins, which include CD29, bind to several cell surface (e.g. VCAM-1, MadCAM-1) and extracellular matrix molecules. CD29 acts as a fibronectin receptor and is involved in a variety of cell-cell and cell-matrix interactions.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC - Verified
IHC, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen tissue sections3,5, and activation of integrin ß14,7,8. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 303010). Clone TS2/16 recognizes epitope A2.10

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Gutierrez-Lopez M, et al. 2003. J. Biol. Chem. 278:208.
  3. Hemler ME, et al. 1984. J. Immunol. 132:3011. (IHC, IP)
  4. Sanchez-Aparicio P, et al. 1994. J. Cell Biol. 126:271. (Activ)
  5. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC)
  6. Murga M, et al. 2005. Blood 105:1992. (FC) PubMed
  7. Porter JC and Hogg N. 1997. J. Cell Biol. 138:1437. (Activ)
  8. Conway RE, et al. 2006. Mol. Cell. Biol. 26:5310. (Activ)
  9. Wesseling J, et al. 1995. J. Cell. Biol. 129:255. (Dog Reactivity)
  10. Rubio G, et al. 2002. Cancer Immunol. Immunother. 51:130.
  11. Dong A, et al. 2015. J Biol Chem. 290:8016. PubMed
  12. Paebst F, et al. 2014. Cytometry A. 85(8):678-87. (Horse reactivity)
Product Citations
  1. Mendt M, et al. 2018. JCI Insight. 9:1523. PubMed
  2. Olin A, et al. 2018. Cell. 174:1277. PubMed
  3. Cho YY, et al. 2019. PLoS One. 14:e0210535. PubMed
  4. Ran LJ, et al. 2018. Mol Med Rep. 18:723. PubMed
  5. Horwacik I, et al. 2017. Int J Oncol. 50:1899. PubMed
  6. Tanigawa K, et al. 2019. J Cell Physiol. 234:17280. PubMed
  7. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  8. Novoseletskaya E, et al. 2020. Front Cell Dev Biol. 8:555378. PubMed
  9. Conway R, et al. 2006. Mol Cell Biol. 26:5310. PubMed
  10. Pillozzi S, et al. 2011. Blood. 117:902. PubMed
  11. Zhang X, et al. 2011. Am J Physiol Cell Physiol. 301:1017. PubMed
  12. Dong A, et al. 2015. J Biol Chem. 290:8016. PubMed
  13. Sivasubramaniyan K, et al. 2015. Glycobiology. 25: 902-917. PubMed
  14. Blázquez-Prunera A, et al. 2017. Stem Cell Res Ther. 10.1186/s13287-017-0552-z. PubMed
  15. Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
  16. Henrick BM, et al. 2021. Cell. . PubMed
  17. Freeman SA, et al. 2020. Science. 367:301. PubMed
  18. Rogers MA, et al. 2022. Front Cardiovasc Med. 9:778919. PubMed
  19. Ceccarelli S, et al. 2022. Front Cell Dev Biol. 10:926180. PubMed
  20. Friedman D, et al. 2021. J Cell Sci. 134:. PubMed
  21. Pilbauerova N, et al. 2021. Int J Mol Sci. 22:. PubMed
RRID
AB_314317 (BioLegend Cat. No. 303001)
AB_314318 (BioLegend Cat. No. 303002)

Antigen Details

Structure
Integrin, type I glycoprotein, forms VLA-1 to VLA-6 heterodimers with CD49a-f (α1-α6), also associates with CD51 (αV), and α7- α9, 130 kD
Distribution

Lymphocytes, monocytes, granulocytes (low), platelets, mast cells, fibroblasts, endothelial cells

Function
Cell-cell and cell-matrix interactions
Ligand/Receptor
VCAM-1, MAdCAM-1, ECM
Cell Type
Embryonic Stem Cells, Endothelial cells, Fibroblasts, Granulocytes, Lymphocytes, Mast cells, Mesenchymal Stem Cells, Monocytes, Platelets, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Hemler M. 1990. Annu. Rev. Immunol. 8:365.
2. Hynes R. 1992. Cell 69:11.

Gene ID
3688 View all products for this Gene ID
UniProt
View information about CD29 on UniProt.org
Go To Top Version: 7    Revision Date: 08.31.2022

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