- Clone
- BM16 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Chemoattractant receptor-homologous molecule expressed on Th2 cells, GPR44, G-protein coupled receptor 44, DP2
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![BM16_AF647_1_081910 BM16_AF647_1_081910](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/BM16_AF647_1_081910.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes stained with CCR4 PerCP/Cy5.5 and purified BM16 conjugated with Alexa Fluor® 647 (top) or rat IgG2a Alexa Fluor® 647 isotype control (bottom) (gated on CD4+ lymphocyte population) -
Human Th2 polarized CD4+ T cells stained with purified BM16 conjugated with Alexa Fluor® 647 -
Human Th2 polarized CD4+ T cells stained with purified BM16 conjugated with Alexa Fluor® 647
Cat # | Size | Price | Quantity Check Availability | Save | ||
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350102 | 100 µg | 126 CHF |
CD294, also known as CRTH2, is a seven-transmembrane protein coupled with heterotrimeric G proteins. CRTH2 is the prostaglandin D2 receptor and is expressed by Th2 cells, eosinophils, and basophils. CD294 prevents the apoptosis of Th2 cells and mediates the chemotaxis of CRTH2 expressing cells to the sites of allergic inflammation, such as the asthmatic lung.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Rat cell line TART/B19-12.10 transfected with human CRTH2
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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This product is sold under license agreement with BML, Inc for research use only.
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Application References
(PubMed link indicates BioLegend citation) -
- Nagata K, et al. 1999. J. Immunol. 162:1278. (FC)
- De Fanis U, et al. 2007. Blood 109:4343. (FC)
- Tsuda H, et al. 2001. Clin. Exp. Immunol. 123:105. (FC)
- Hamada K, et al. 2004. Allergology International 53:179. (FC)
- Product Citations
- RRID
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AB_10639863 (BioLegend Cat. No. 350102)
Antigen Details
- Structure
- Seven-transmembrane protein coupled with G proteins
- Distribution
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Effector T helper 2 (Th2) cells, eosinophils, basophils
- Function
- Chemotaxis of eosinophils, basophils and Th2 cells, prevention of apoptosis in Th2 cells
- Interaction
- Heterotrimeric G proteins
- Ligand/Receptor
- Prostaglandin D2
- Cell Type
- Th2, Eosinophils, Basophils
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
- Molecular Family
- CD Molecules
- Antigen References
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1. Luster AD and Tager AM. 2004. Nat. Rev. Immunol. 4:711.
2. Monneret G, et al. 2001. Blood 98:1942.
3. Xue L, et al. 2009. J. Immunol. 182:7580.
4. Schratl P, et al. 2007. J. Immunol. 179:4792. - Gene ID
- 11251 View all products for this Gene ID
- UniProt
- View information about CD294 on UniProt.org
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