- Clone
- UCHT1 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- III 471
- Other Names
- T3, CD3ε
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1_UCHT1_Purified_021805 1_UCHT1_Purified_021805](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/UCHT1_Purified_021805.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes stained with purified UCHT1 and then detected with anti-mouse IgGs FITC -
Human frozen spleen tissue slices were fixed with 4% PFA for ten minutes and blocked with 5% FBS for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD3 antibody (clone UCHT1, red) overnight at 4°C. On the next day, tissue was incubated with Alexa Fluor® 594 Goat anti-mouse IgG (clone Poly4053, red). Nuclei were counter-stained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
300401 | 25 µg | 24 CHF | ||||
300402 | 100 µg | 44 CHF |
CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
CyTOF®, IHC-F - Verified
IP, Activ, WB - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
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Application References
(PubMed link indicates BioLegend citation) -
- Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
- Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
- Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
- Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
- Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
- Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
- Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
- Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
- Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
- Li J, et al. 2015. Cancer Res. 75:508. PubMed
- Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
- Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
- RRID
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AB_314055 (BioLegend Cat. No. 300401)
AB_314056 (BioLegend Cat. No. 300402)
Antigen Details
- Structure
- Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
- Distribution
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Mature T and NK T cells, thymocyte differentiation
- Function
- Antigen recognition, signal transduction, T cell activation
- Ligand/Receptor
- Peptide antigen bound to MHC
- Cell Type
- NKT cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, TCRs
- Antigen References
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1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507. - Gene ID
- 916 View all products for this Gene ID
- UniProt
- View information about CD3 on UniProt.org
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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