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Purified anti-human CD39 Antibody

Pricing & Availability
Clone
A1 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
gp80, E-ATPDase, NTPDase-1, ecto-apyrase, Ec3.6.1.5
Isotype
Mouse IgG1, κ
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Product Citations
publications
A1_Pure_011408
Human peripheral blood lymphocytes stained with purified A1, followed by anti-mouse IgG FITC
  • A1_Pure_011408
    Human peripheral blood lymphocytes stained with purified A1, followed by anti-mouse IgG FITC
Cat # Size Price Quantity Check Availability Save
328202 100 µg 112 CHF
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Description

Human CD39 is an integral membrane protein with two transmembrane domains. It exists as a homotetramer. Expression of CD39 is found on activated lymphocytes, a subset of T cells and B cells, and dendritic cells with weak staining on monocytes and granulocytes. CD39 and CD73 have been found on regulatory T cells, specifically the effector/memory like T cells. CD39 can hydrolyze both nucleoside triphosphates and diphosphates. CD39 is the dominant ecto nucleotidase of vascular and placental trophoblastic tissues and appears to modulate the functional expression of type 2 purinergic (P2) G protein coupled receptors (GPCRs). CD39 has intrinsic ecto-ATPase activity. Expression of CD39 is induced on T cells and increased on B cells as a late activation antigen.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
PHA activated human lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P, Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The A1 antibody binds to the human CD39 cell surface antigen and has been shown to block MHC independent target cell recognition by hapten-specific CTL. Additional reported applications (for the relevant formats) include: in vitro CD39 blockade3,  immunofluorescence4, immunohistochemistry6, and spatial biology (IBEX)7,8. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for blocking assays (contact our custom solutions team).

Application References

(PubMed link indicates BioLegend citation)
  1. Aversa GG, et al. 1988. Transplant. P. 20:4952.
  2. Aversa GG, et al. 1989. Transplant. P. 21:34950.
  3. Borsellino G, et al. 2007. Blood. 110:1225. (Block)
  4. Stockl J, et al. 2001. J. Immunol. 167:2724. (IF)
  5. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
  6. Lyck L, et al. 2008. J. Histochem. Cytochem. 56:201. (IHC)
  7. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  8. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
  2. Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed
  3. Azizi E et al. 2018. Cell. 174(5):1293-1308 e36. PubMed
  4. Olin A, et al. 2018. Cell. 174:1277. PubMed
  5. Brinkmann CR, et al. 2018. mSphere. 3:e00616. PubMed
  6. Lakshmikanth T, et al. 2020. Cell Reports. 32(3):107923. PubMed
  7. Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
  8. Henrick BM, et al. 2021. Cell. . PubMed
  9. Schwabenland M, et al. 2021. Immunity. . PubMed
  10. Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed
  11. NULL, et al. 2022. Cell. 185:916. PubMed
  12. Syrimi E, et al. 2021. iScience. 24:103215. PubMed
  13. O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed
  14. Harshe RP, et al. 2020. Nat Commun. 11:5894. PubMed
  15. Leader AM, et al. 2021. Cancer Cell. 39:1594. PubMed
  16. McIlwain DR, et al. 2021. Cell Host Microbe. 29:1828. PubMed
  17. Glass MC, et al. 2022. Cell Rep. 39:110728. PubMed
  18. Diray-Arce J, et al. 2023. Cell Rep Med. 4:101079. PubMed
RRID
AB_940438 (BioLegend Cat. No. 328202)

Antigen Details

Distribution

Activated lymphocytes, and also on a subset of T cells, regulatory T cells, B cells, and dendritic cells.

Cell Type
B cells, Dendritic cells, Lymphocytes, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
953 View all products for this Gene ID
UniProt
View information about CD39 on UniProt.org
Go To Top Version: 4    Revision Date: 08.15.2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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