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Purified anti-human CD45 Antibody

Pricing & Availability
Clone
HI30 (See other available formats)
Regulatory Status
RUO
Workshop
IV N816
Other Names
LCA, T200
Isotype
Mouse IgG1, κ
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Product Citations
publications
HI30_Purified_071307
Human peripheral blood lymphocytes were stained with purified CD45 (clone HI30) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
  • HI30_Purified_071307
    Human peripheral blood lymphocytes were stained with purified CD45 (clone HI30) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
  • HI30_Purified_2_CD45_IHC_071315
    Preparation and staining of formalin fixed paraffin-embedded (FFPE) human tonsil was performed. After antigen retrieval, the sample was incubated with the purified monoclonal antibody (clone HI30) at 0.5 µg/mL for 20 minutes. The Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB) was used according to protocol for chromogenic detection.
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304001 25 µg 24 CHF
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304002 100 µg 50 CHF
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Description

CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF®, IHC-P - Verified
WB, IHC-F, ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.1 - 10 µg/ml is suggested, if the antibody is not available in a pre-diluted format. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11, Western blotting3, and spatial biology (IBEX)16,17.

It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Kishihara K, et al. 1993. Cell 74:143.
  3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
  4. Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
  5. Nagano M, et al. 2007. Blood 110:151.
  6. Jiang Q, et al. 2008. Blood 112:2858. PubMed
  7. Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  10. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
  11. Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
  12. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  13. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
  14. Marquardt N, et al. 2015. J Immunol. 6:2467. PubMed
  15. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Gunawan M, et al. 2017. Sci Rep. . 10.1038/s41598-017-16999-7. PubMed
  2. Chevrier S, et al. 2018. Cell Syst. 0.675. PubMed
  3. Behrens NE, et al. 2019. MBio. 10:2. PubMed
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  6. Staessens S, et al. 2019. Haematologica. 104(10). PubMed
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  12. Cao Q, et al. 2018. Am J Physiol Renal Physiol. 314:F561. PubMed
  13. Eldredge LC, et al. 2019. Am J Physiol Lung Cell Mol Physiol. 317:L49. PubMed
  14. Böttcher C, et al. 2019. Sci Rep. 9:19471. PubMed
  15. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  16. Borges TJ, et al. 2018. Nat Commun. 9:3482. PubMed
  17. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
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  19. Frobel J, et al. 2018. Clin Epigenetics. 10:67. PubMed
  20. Kuwada K, et al. 2018. J Exp Clin Cancer Res. 37:307. PubMed
  21. Bokun V, et al. 2019. PLoS One. 14:e0225767. PubMed
  22. Li Y, et al. 2019. Front Immunol. 0.460416667. PubMed
  23. Chen M, et al. 2019. Cell Stem Cell. 25:501. PubMed
  24. Chabi S, et al. 2020. Cell Reports. 29(8):2307-2320.e6.. PubMed
  25. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
  26. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  27. Nagano M, et al. 2007. Blood . 110:151. PubMed
  28. Jiang Q, et al. 2008. Blood. 112:2858. PubMed
  29. Bhat N, et al. 2015. J Immunol. 195: 5178 - 5188. PubMed
  30. Hu Y, et al. 2016. J Exp Med. 213: 2759 - 2772. PubMed
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  32. Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
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  35. Schwabenland M, et al. 2021. Immunity. . PubMed
  36. Wastyk HC, et al. 2021. Cell. 184(16):4137-4153.e14. PubMed
  37. Szodoray P, et al. 2021. Cell Reports. 36(6):109525. PubMed
  38. Kanayama M, et al. 2021. Oncol Lett. 22:522. PubMed
  39. Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed
  40. Chiou SH, et al. 2021. Immunity. 54:586. PubMed
  41. Nagasawa Y, et al. 2021. PLoS One. 16:e0249340. PubMed
  42. Korn MA, et al. 2020. J Immunol. 205:2595. PubMed
  43. Zheng Y, et al. 2022. iScience. 25:103785. PubMed
  44. Gañán-Gómez I, et al. 2022. Nat Med. . PubMed
  45. Elst J, et al. 2022. Front Immunol. 13:835618. PubMed
  46. Crawford LB, et al. 2021. J Virol. 95:. PubMed
  47. Li H, et al. 2020. Haematologica. 105:1206. PubMed
  48. Chow SH, et al. 2020. J Leukoc Biol. 108:967. PubMed
  49. Jowett GM, et al. 2021. Nat Mater. 20:250. PubMed
  50. Woloshuk A, et al. 2021. Cytometry A. 99:707. PubMed
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  55. Liu C, et al. 2022. Regen Ther. 21:192. PubMed
  56. Lee SA, et al. 2020. J Immunol. 204:586. PubMed
  57. Cox MJ, et al. 2021. Mol Ther. 29:1529. PubMed
  58. Rabilloud T, et al. 2021. Nat Commun. 12:865. PubMed
  59. Sugimoto H, et al. 2021. AAPS J. 23:36. PubMed
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RRID
AB_314389 (BioLegend Cat. No. 304001)
AB_314390 (BioLegend Cat. No. 304002)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)
Distribution

Hematopoietic cells, not expressed in circulating erythrocytes or platelets

Function
TCR and BCR mediated activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
Hematopoietic stem and progenitors, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
  2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org
Go To Top Version: 5    Revision Date: 08.09.2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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