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Purified anti-human CD8 Antibody

Pricing & Availability
Clone
SK1 (See other available formats)
Regulatory Status
RUO
Other Names
T8, Leu2
Isotype
Mouse IgG1, κ
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Product Citations
publications
SK1_FITC_092409
Human peripheral blood lymphocytes stained with SK1, followed by anti-mouse IgG FITC.
  • SK1_FITC_092409
    Human peripheral blood lymphocytes stained with SK1, followed by anti-mouse IgG FITC.
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344702 100 µg 141 CHF
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Description

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics8, immunohistochemistry of acetone-fixed frozen tissue sections, and spatial biology (IBEX)9,10. This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter JA, et al. 1981. J. Exp. Med. 153:310.
  2. Campanelli R, et al. 2002. Intl. Immunol. 14:39.
  3. Evans RL, et al. 1981. Immunol. 78:544.
  4. Wooldridge L, et al. 2005. J. Bio. Chem. 280:27491.
  5. Ch'el IL, et al. 2011. J Exp Med. 208:633. PubMed
  6. Carbone A, et al. 1999. Blood 93:2319. (IHC-F)
  7. Ahmed A, et al. 2001. J. Pathol. 193:383. (IHC)
  8. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  9. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  10. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Gunawan M, et al. 2017. Sci Rep. . 10.1038/s41598-017-16999-7. PubMed
  2. Santos R, et al. 2017. Nat Commun. . 10.1038/s41467-017-01760-5. PubMed
  3. Olin A, et al. 2018. Cell. 174:1277. PubMed
  4. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  5. Zhao J, et al. 2017. Sci Immunol. 2:eaan5400. PubMed
  6. Geng J, et al. 2018. Elife. 7:e36341. PubMed
  7. Nguyen LT, et al. 2019. Cancer Immunol Immunother. 68:773. PubMed
  8. Yoshihara S, et al. 2019. Front Immunol. 0.545833333. PubMed
  9. Bak S, et al. 2019. Front Immunol. 9:2953. PubMed
  10. Lakshmikanth T, et al. 2020. Cell Reports. 32(3):107923. PubMed
  11. Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed
  12. Park JB, et al. 2019. Mol Cells. 42:597. PubMed
  13. Oberg HH, et al. 2020. J Leukoc Biol. 107:1081. PubMed
  14. Li H, et al. 2020. Mol Ther Methods Clin Dev. 1.436111111. PubMed
  15. Xie CB, et al. 2020. J Clin Invest. 130:3437. PubMed
  16. Henrick BM, et al. 2021. Cell. . PubMed
  17. Mann ER, et al. 2020. Sci Immunol. :5. PubMed
  18. Cosgrove PR, et al. 2021. Pediatr Res. . PubMed
  19. Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed
  20. Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed
  21. Chiou SH, et al. 2021. Immunity. 54:586. PubMed
  22. Liu F, et al. 2020. J Immunother Cancer. 8:. PubMed
  23. Ravell JC, et al. 2020. J Clin Invest. 130:507. PubMed
  24. Jilg N, et al. 2020. J Infect Dis. 222:1837. PubMed
  25. Yamaguchi A, et al. 2021. J Thorac Dis. 13:5430. PubMed
  26. Cobb DA, et al. 2022. J Immunother Cancer. 10:. PubMed
  27. Moon JS, et al. 2023. Nat Commun. 14:319. PubMed
RRID
AB_1877104 (BioLegend Cat. No. 344702)

Antigen Details

Structure
Ig superfamily, homodimer or heterodimer with CD8b, 32-34 kD
Distribution

Majority of thymocytes, T cell subset, NK cells

Function
MHC class I co-receptor, thymic differentiation, T cell activation
Ligand/Receptor
MHC Class I molecules
Cell Type
NK cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.

Gene ID
925 View all products for this Gene ID
UniProt
View information about CD8 on UniProt.org
Go To Top Version: 8    Revision Date: 09.01.2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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