- Clone
- TY/11.8 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ecto-5'-nucleotidase
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![TYslash11.8_PURE_CD73_Antibody_Dot_071108. TYslash11.8_PURE_CD73_Antibody_Dot_071108.](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/TYslash11_8_Pure_071108.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes stained by CD3 (145-2C11) APC and purified TY/11.8, followed by anti-rat IgG biotin and SAV-PE -
C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of purified anti-mouse CD73 (clone TY/11.8) and 10 µg/mL of Alexa Fluor® 647 anti-mouse CD3ε (clone 145-2C11) (green) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 594 anti-rat IgG (clone Poly4054) (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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127202 | 100 µg | 106 CHF |
CD73 (ecto-5'-nucleotidase) is a 69 kD GPI-anchored surface protein. In mice, expression of CD73 in bone marrow is restricted to CD11b+ myeloid cells. In spleen, it is largely expressed on T cells.
Product DetailsProduct Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- CHO cells transfected with mouse CD73
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
IHC-F - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
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Application References
(PubMed link indicates BioLegend citation) -
- Yamshita Y, et al 1998. Eur. J. Immunol. 28:2981.
- Bentley JK, et al. 2010. Respir. Res. 11:127. (IHC-P)
- Product Citations
- RRID
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AB_1089066 (BioLegend Cat. No. 127202)
Antigen Details
- Structure
- 69 kD GPI-anchored surface protein
- Distribution
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Positive on most CD11b+ myeloid cells in bone marrow, but not on CD11b+ peripheral cells; a large proportion of CD4+ and CD8+ splenic T cells express CD73
- Function
- A nucleotidase catalyzing the dephosphorylation of extracellular nucleoside 5' monophosphates
- Ligand/Receptor
- Nucleoside 5' monophosphate
- Cell Type
- B cells, Mesenchymal Stem Cells, Tregs
- Biology Area
- Costimulatory Molecules, Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Yamashita Y, et al 1998. Eur. J. Immunol. 28:2981.
2. Resta R, et al. 1998. Immunol. Rev. 161:95. - Regulation
- Expression is regulated by the development and maturation of leukocytes
- Gene ID
- 23959 View all products for this Gene ID
- UniProt
- View information about CD73 on UniProt.org
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