- Clone
- 3A2A20 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- POU domain class 5, transcription factor 1 (POU5F1), OCT4, OCT3
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1_3A2A20_BV421_Oct4_Antibody_ICC_080219 1_3A2A20_BV421_Oct4_Antibody_ICC_080219](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/3A2A20_BV421_Oct4_Antibody_ICC_080219.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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NCCIT pluripotent embryonal carcinoma cells were fixed with 4% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml anti-Oct4 (Oct3) (clone 3A2A20) Brilliant Violet 421™ (blue) in 5% FBS overnight at 4°C and followed by Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes at 4°C. Nuclei were counterstained with DRAQ5 (red). The image was captured with a 40X objective. -
Human teratocarcinoma cell line NCCIT cells were fixed and permeabilized with BioLegend’s FOXP3 Fix/Perm Buffer Set, and then stained with anti-Oct4 (clone 3A2A20) Brilliant Violet 421™ (filled histogram) or mouse IgG2b, κ Brilliant Violet 421™ isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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653712 | 100 µg | 435 CHF |
Oct4 (Octamer binding transcription factor 4) is an important marker of the undifferentiated state and central regulator of pluripotency in ES cells. When embryonic stem cells are triggered to differentiate, Oct4 is downregulated, thus providing a model for the early events linked to somatic differentiation in the developing embryo. Oct4 is a nuclear protein, with a predicted molecular weight of 39 kD.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial human Oct4 recombinant protein (1-141aa).
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
ICFC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 10 μg/ml is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 μg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- RRID
-
AB_2566690 (BioLegend Cat. No. 653712)
Antigen Details
- Structure
- Belongs to the POU transcription factor family, predicted molecular weight of 39 kD
- Distribution
-
Nuclear protein, expressed in undifferentiated embryonal carcinoma (EC) cells, embryonic stem (ES) cells and embryonic germ (EG) cells
- Function
- Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Oct4 is a prime candidate for an early developmental control gene. It also is an important marker of the undifferentiated state and central regulator of pluripotency in ES cells.
- Interaction
- SOX2, SOX8, SOX10, androgen receptor, and ETS2
- Biology Area
- Cell Biology, Immunology, Stem Cells, Transcription Factors
- Antigen References
-
1. Niwa H, et al. 2000. Nature Genet. 24:372.
2. Wernig M, et al. 2007. Nature 448:318.
3. Pesce M, et al. 2001. Stem Cells 19:271. - Gene ID
- 5460 View all products for this Gene ID
- UniProt
- View information about Oct4 on UniProt.org
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
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It is lot-specific. On average it ranges between 2-4.
Other Formats
View All Oct4 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Oct4 (Oct3) | 3A2A20 | WB,ICC,ChIP |
PE anti-Oct4 (Oct3) | 3A2A20 | ICFC |
Alexa Fluor® 488 anti-Oct4 (Oct3) | 3A2A20 | ICFC |
Brilliant Violet 421™ anti-Oct4 (Oct3) | 3A2A20 | ICC,ICFC |
Alexa Fluor® 647 anti-Oct4 (Oct3) | 3A2A20 | ICC,ICFC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Oct4 (Oct3)
Total lysates (15 µg protein) from NTERA2 (lane 1), HeLa (la... NTERA-2 cells were fixed with 4% paraformaldehyde (PFA) for ... Chromatin Immunoprecipitations (ChIP) were performed with cr... Chromatin Immunoprecipitations (ChIP) were performed with cr... -
PE anti-Oct4 (Oct3)
Human teratocarcinoma cell line, NCCIT, was treated with FOX... -
Alexa Fluor® 488 anti-Oct4 (Oct3)
Human teratocarcinoma cell line NCCIT cells were fixed and p... -
Brilliant Violet 421™ anti-Oct4 (Oct3)
NCCIT pluripotent embryonal carcinoma cells were fixed with ... Human teratocarcinoma cell line NCCIT cells were fixed and p... -
Alexa Fluor® 647 anti-Oct4 (Oct3)
NCCIT pluripotent embryonal carcinoma cells were fixed with ... Human teratocarcinoma cell line NCCIT cells were fixed and p...
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