- Clone
- 6-His (See other available formats)
- Regulatory Status
- RUO
- Other Names
- polyhistidine-tag, hexa histidine-tag, 6xHis-tag, His6 tag, His-tag(TM)
- Previously
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Covance Catalog# MMS-156P
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1_6-His_Purified_6-His_Antibody_WB_021715 1_6-His_Purified_6-His_Antibody_WB_021715](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/6-His_Purified_6-His_Antibody_WB_021715.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western Blot showing specificity of 6-His Monoclonal Antibody, Purified, 1:900. Lane 1: MW Marker, Lane 2: PosiTag lysate, Lane 3: Hela cell lysate negative control. -
Hela cells transfected with multiple tag plasmid were fixed with cold methanol at -20°C for 15 minutes and blocked with 5% FBS for 60 minutes. The cells were then intracellularly stained with either (A) 2 µg/mL anti-mouse IgG1, κ or (B) 2 µg/mL anti-6-His antibody (clone 6-His), or (C) 0.5 µg/mL anti-6-His antibody (clone 6-His), or (D) 0.2 µg/mL anti-6-His antibody (clone 6-His), and incubated overnight at 4°C followed by 2µg/ml (1:250) Alexa Fluor® 594 (Red) goat anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI (Blue). The image was captured with a 60X objective. Exposure times: (A) 1/25, (B) 1/80, (C) 1/80, (D) 1/80.
His tags are commonly used as a method of tagging proteins for detection or purification. This antibody is raised again a polyhistidine tag (6-His or hexa His-tag).
Product DetailsProduct Details
- Verified Reactivity
- Epitope tag
- Reported Reactivity
- Species independent
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Monoclonal 6-His antibody was raised against a recombinant protein containing the sequence HHHHHH.
- Formulation
- Phosphate-buffered solution + 0.03% Thimerosal.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/mL
- Storage & Handling
- Store between 2°C and 8 °C.
- Application
-
WB - Quality tested
ICC, IP - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:1000 dilution. For immunocytochemistry, the suggested use is 1:1000 – 1:4000 dilution. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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The sequence of the protein flanking the poly His tag does not influence antibody binding. This antibody was subsequently purified via a protein-G column.
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Application References
(PubMed link indicates BioLegend citation) -
- Deinert K, et al. 2001. J. Biol. Chem. 276:6000. (WB)
- Kojima H, et al. 2001. J. Biol. Chem.(22)276:19238. (IF) PubMed
- Lapinski PE, et al. 2000. J. Biol. Chem. 275:6831. (WB)
- Bartsch D, et al. 1998. Cell. 95:211. (IF)
- Fuentes EJ, et al. 2003. J Biol Chem. 278:2118. PubMed
- Montegna EA, et al. 2012 PLoS One.7:31156. (WB) PubMed
- Huang L, et al. 2013. Nat Biotechnol. 31:450. (WB) PubMed
- Product Citations
-
- RRID
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AB_2734683 (BioLegend Cat. No. 906113)
AB_2565061 (BioLegend Cat. No. 906101)
AB_2565062 (BioLegend Cat. No. 906102)
Antigen Details
- Biology Area
- Cell Biology
- Gene ID
- NA
- UniProt
- View information about 6-His on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All 6-His Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Anti-6-His Epitope Tag Affinity Matrix | 6-His | IP,Purification |
Biotin anti-6-His Epitope Tag | 6-His | WB,ICC,IP |
FITC anti-6-His Epitope Tag | 6-His | ICC |
Purified anti-6-His Epitope Tag | 6-His | WB,ICC,IP |
Direct-Blot™ HRP anti-6-His Epitope Tag | 6-His | WB |
Alexa Fluor® 647 anti-6-His Epitope Tag | 6-His | ICC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Anti-6-His Epitope Tag Affinity Matrix
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Biotin anti-6-His Epitope Tag
Western blot analysis of 0.5 µl Posi-tag (Cat. No. 931301) u... Immunofluorescence of His tagged plasmid transfected A549 ce... -
FITC anti-6-His Epitope Tag
ICC staining of FITC anti-6-His Epitope Tag antibody (clone ... -
Purified anti-6-His Epitope Tag
Western Blot showing specificity of 6-His Monoclonal Antibod... Hela cells transfected with multiple tag plasmid were fixed ... -
Direct-Blot™ HRP anti-6-His Epitope Tag
Western blot analysis of 15 µg cell lysates from HeLa cells ... -
Alexa Fluor® 647 anti-6-His Epitope Tag
HeLa cells were transfected with (B-D) or without (A) His-ta...
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