- Clone
- A16097D (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Microtubule associated protein tau
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A16097D_Purified_Tau419-433_Antibody_1_072718 A16097D_Purified_Tau419-433_Antibody_1_072718](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A16097D_Purified_Tau419-433_Antibody_1_072718.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of purified anti-Tau, 419-433 antibody (clone A16097D). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-rat IgG secondary antibody (Cat. No. 405405). Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system. -
IHC staining of anti-Tau, 419-433 antibody (clone A161097D) and rat IgG2a isotype control on formalin-fixed paraffin-embedded Alzheimer’s disease brain tissue. Following antigen retrieval using Sodium Citrate, the tissues were incubated with anti-Tau or rat IgG2a at 0.2 µg/ml for 1 hour at room temperature. Biotinylated anti-rat IgG, HRP Streptavidin, and DAB (3,3'-diaminobenzidine) substrate were used as the detection system. Slides were counterstained with hematoxylin. -
Direct ELISA of purified anti-Tau, 419-433 antibody (clone A16097D) and rat IgG2a isotype control binding to plate-immobilized recombinant human 2N3R and 2N4R Tau proteins. ELISA was performed by coating wells with 100 ng of each recombinant Tau protein. The wells were then incubated with anti-Tau or rat IgG2a at 37°C for 45 minutes, followed by incubation with horseradish peroxidase labeled goat anti-rat secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine) was used as the detection system.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
851001 | 25 µg | 112 CHF | ||||
851002 | 100 µg | 276 CHF |
Tau protein promotes microtubule assembly and stability. Tau is abundant in neurons of the central nervous system, and is expressed at low levels in astrocytes and oligodendrocytes. Abnormal hyper-phosphorylation, aggregation, and toxic gain of function of tau is associated with several neurological disorders, including Alzheimer’s disease (AD). The major building block of neurofibrillary lesions in AD brains consists of paired helical filaments (PHFs) of abnormally hyperphosphorylated tau. Six isoforms of tau are generated by alternative splicing of the MAPT gene. These isoforms are distinguished by the number of tubulin binding domains, 3 (3R) or 4 (4R), in the C-terminal of the protein and by one (1N), two (2N), or no (0N) inserts in the N-terminal domain. Tau isoforms are differentially expressed during development.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant C-terminal fragment of human 4R Tau protein encompassing amino acid residues 419-433.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P, Direct ELISA - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg/ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.2 - 2.0 µg/ml is suggested. For Direct ELISA, a concentration range of 0.01 - 1 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody cross-reacts with 3R and 4R Tau isoforms, and recognizes endogenous human and mouse Tau in brain lysates by WB.
- RRID
-
AB_2721764 (BioLegend Cat. No. 851001)
AB_2721765 (BioLegend Cat. No. 851002)
Antigen Details
- Structure
- Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.
- Distribution
-
Tissue distribution: central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle.
Cellular distribution: cytoskeleton, nucleus, plasma membrane and cytosol. - Function
- Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
- Interaction
- Tau interacts with Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine/threonine-protein kinase Sgk1, Laforin, Alpha-synuclein
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- Tau
- Antigen References
- Gene ID
- 4137 View all products for this Gene ID
- UniProt
- View information about Tau 419-433 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All Tau, 419-433 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Tau, 419-433 | A16097D | WB,Direct ELISA,IHC-P |
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