Recombinant Human TPO (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
Megakaryocyte Colony Stimulating Factor (MKCSF), Myeloproliferative leukemia virus oncogene ligand (Mpl-ligand), Megakaryocyte growth and development factor (MGDF), THPO
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Product Citations
publications
Human_TPO_RECOM_CF_042517
Human TPO induces proliferation of MO7e megakaryocytic leukemia cells in a dose dependent manner with ED50 of 0.15 - 0.6 ng/mL.
  • Human_TPO_RECOM_CF_042517
    Human TPO induces proliferation of MO7e megakaryocytic leukemia cells in a dose dependent manner with ED50 of 0.15 - 0.6 ng/mL.
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763702 10 µg 218 CHF
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763704 25 µg 317 CHF
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763706 100 µg 996 CHF
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Description

TPO is a key regulator of megakaryocytopoiesis and thrombopoiesis. TPO binds to its receptor, the cellular homologue of the myeloproliferative leukemia virus oncogene (c-Mpl), and stimulates the proliferation and maturation of megakaryocytes. MPL does not possess kinase activity, thus the receptor associates with intracytoplasmic tyrosine kinases, janus kinase 2 (JAK2), for signal transduction.  JAK2 is also important for MPL stability and cell-surface expression. TPO is able to promote the survival, self-renewal, and expansion of hematopoietic stem cells and primitive multi-lineage progenitor cells. TPO levels in blood and bone marrow are inversely related to platelet count.  The regulation of TPO levels is mediated by its receptor c-Mpl (uptake and destruction). TPO is upregulated by PDGF and FGF-2, and it is downregulated by PF4, thrombospondin, and TGF-β in bone marrow stromal cells. In hepatocytes, HGF enhances TPO mRNA expression. During acute-phase response, IL-6 induces TPO transcription in the liver. Elevated plasma TPO levels exist in several hematological diseases associated with thrombocytopenia, coronary syndromes, and sepsis. Besides its hematopoietic effects, TPO is expressed in the brain where it promotes apoptosis of hypoxia sensitized neurons and inhibits neuronal differentiation by blocking NGF-induced signaling. Increased TPO concentrations are present in the cerebrospinal fluid of some patients with bacterial or viral meningitis.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human TPO, (amino acids Ser22-Gly353, Accession # NM_000460) was expressed in 293E cells.
Molecular Mass
The 332 amino acid recombinant protein has a predicted molecular mass of approximately 35.5 kDa. The DTT-reduced and non-reduced proteins migrate at approximately 70 kDa by SDS-PAGE. The predicted N-terminal amino acid is Ser.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2.
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
ED50 = 0.15 - 0.6 ng/mL as measured by the ability of protein to induce proliferation of MO7e megakaryocytic leukemia cells. Deep Blue Cell Vialibity Kit was used to quantitate cell proliferation.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Product Citations
  1. Woodley K, et al. 2023. Nat Commun. 14:2132. PubMed

Antigen Details

Structure
Growth Factor, homodimer
Distribution

TPO is produced by the liver and kidney and is expressed in the central nervous system and bone marrow

Function
TPO regulates the megakaryocytopoiesis and thrombopoiesis
Interaction
Megakaryocytes, platelets, hemangioblasts, hematopoietic stem cells, and endothelial cells
Ligand/Receptor
c-Mpl
Bioactivity
Measured by its ability to induce proliferation of MO7e megakaryocytic leukemia cells
Cell Type
Mesenchymal Stem Cells, Hematopoietic stem and progenitors, Embryonic Stem Cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Stem Cells
Molecular Family
Growth Factors, Cytokines/Chemokines
Antigen References

1. Avanzi G, et al. 1988. Br. J. Haematol. 69:359.
2. Deutsch V.R. and Tomer A. 2006. Br. J. Haematol. 134:453.
3. Drachman JG, et al. 1995. J. Biol. Chem. 270:4979.
4. Li J. et al. 1999. Br. J. Haematol. 106:345.
5. Kato T, et al. 1997. Proc. Natl. Acad. Sci. 94:4669.
6. Ehrenreich H, et al. 2005. Proc. Natl. Acad. Sci. USA. 102:862.
7. Royer Y, et al. 2005. J. Biol. Chem. 280:27251.
8. Kaushansky K. 2005. J. Clin. Invest. 115:3339.
9. de Graaf CA and Metcalf D. 2011. Cell Cycle 10:1582.
10. Zhang J, et al. 2010. J. Interferon Cytokine Res. 7:465.
11. Lupia E, et al. 2012. Mediators Inflamm. 2012:390892.
12. Besancenot R, et al. 2014. Blood 124:2104.

Gene ID
7066 View all products for this Gene ID
UniProt
View information about TPO on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 0    Revision Date: 04.25.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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