- Clone
- 16B12 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
- Previously
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Covance Catalog# MMS-101P
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1_16B12_Purified_HA.11_Antibody_1_WB_061915 1_16B12_Purified_HA.11_Antibody_1_WB_061915](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/16B12_Purified_HA.11_Antibody_1_WB_061915.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of Clone 16B12. Lane 1: Molecular weight marker; Lane 2: Posi-tag control Protein (Cat # 931301); Lane 3: HA.11 overexpressing cell lysate; Lane 4: Untransfected cell lysate. -
Transfected RBL1 cells expressing the HA tag on the cell surface were stained with purified anti-HA.11 epitope Tag (clone 16B12) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by APC conjugated goat-anti-mouse IgG. -
Staining of Clone 16B12 on methanol fixed CHO cells transfected with an HA-tagged protein at 1 µg/ml. Cells were then incubated with Alexa Fluor® 594 Goat anti-mouse IgG secondary and were counterstained with DAPI (blue). -
Immunoprecipitation of HA protein from CHO and CHO-HA stable cell extracts using HA Mouse primary antibody. Western blot analysis was performed using Direct-Blot™ HRP anti-HA (#901519). Direct-Blot™ HRP anti-β-actin (#643807) was used as a lysate loading control.
The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106. It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA.
Product DetailsProduct Details
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Monoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL.
- Formulation
- Phosphate-buffered solution + 50% Glycerol + 0.03% Thimerosal.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/mL
- Storage & Handling
- Store between 2°C and 8°C.
- Application
-
WB - Quality tested
FC, ICC, IP - Verified
Purification - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is a dilution of 1:2,000-1:20,000. For immunocytochemistry, the suggested use of this reagent is a dilution of 1:1,000 is suggested. For immunoprecipitation, the suggested use of this reagent is a dilution of 1:50 - 1:100 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional tested and reported applications of the 16B12 clone for the relevant formats include: western blot (WB), immunocytochemistry (ICC), immunoprecipitation (IP), and flow cytometry (FC).
*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.
This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. The HA.11 antibody recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of the antibody allows unambiguous identification and quantitative analysis of the tagged protein. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus. -
Application References
(PubMed link indicates BioLegend citation) -
- Kim JY, et al. 2003. J Neurosci. 23:5561. (IP, WB)
- Helliwell SB, et al. 2001. J Cell Biol. 153:649. (WB)
- Bennett BD, et al. 2000. J Biol Chem. 275:37712. (IF, IP, WB)
- Royer Y, et al. 2005. J. Biol. Chem. 29:27251. (FC)
- Smith BA, et al. 2012. Genes Cancer. 3:550. (IHC) PubMed
- Hogarth C, et al. 2015. Biol Reprod. 93:19. PubMed
- Görtz D, et al. 2015. Sci Rep. 5:14685. PubMed
- Wilson C, et al. 2015. PLoS One. 10:0139579. PubMed
- Smith B, et al. 2012. Genes Cancer. 3:550-563. PubMed
- Liu Z, et al. 2016. Nature. 530:98-102. PubMed
- Thoms M, et al. 2016. Nucleic Acids Res. 44:926-39. PubMed
- Kim Y, et al. 2016. Nat Commun. 7:10347. PubMed
- Rodríguez-Escudero M, et al. 2016. PLoS One. 11:0148032. PubMed
- Lehmann W, et al. 2016. Nat Commun. 7:10498. PubMed
- Testoni E, et al. 2016. EMBO Mol Med. 8: 105-16. PubMed
- Padilla S, et al. 2016. Nat Neurosci. 10.1038/nn.4274. PubMed
- Martins J, et al. 2016. J Cell Sci. 129:1271-82. PubMed
- Matak P, et al. 2016. Proc Natl Acad Sci U S A. 113:3428-35. PubMed
- Starokadomskyy P, et al. 2016. Nat Immunol. 17:495-504. PubMed
- Mitxelena J, et al. 2016. Nucleic Acids Res. 44:5557-70. PubMed
- Thongthip S, et al. 2016. Genes Dev. 30:645-59. PubMed
- Aaes T, et al. 2016. Cell Rep. 15:274-87. PubMed
- Hodge C, et al. 2016. J Biol Chem. 291:9396-9410. PubMed
- Alagramam K, et al. 2016. Nat Chem Biol. 12:444-51. PubMed
- Veit G, et al. 2016. PLoS Biol. 14:1002462. PubMed
- Lee B, et al. 2016. Development. 143:1721-31. PubMed
- Douchi D, et al. 2016. Plant Cell. 28:1182-99. PubMed
- Avgousti D, et al. 2016. Nature. 535:173-77. PubMed
- Shin H, et al. 2015. Nature. 534:553-7. PubMed
- Gross G, et al. 2016. Nat Methods. 10.1038/nmeth.3894. PubMed
- Dick M, et al. 2016. Nat Commun. 7:11929. PubMed
- Aldrin-Kirk P, et al. 2016. Neuron. 90:955-68. PubMed
- Fan R, et al. 2016. Nat Med. 22:780-91. PubMed
- Rowald K, et al. 2016. Cell Rep. 15:2679-91. PubMed
- Rampal R, Awasthi A, Ahuja V 2016. Development. 143:2334-43. PubMed
- Faden F, et al. 2016. Nat Commun. 7:12202. PubMed
- Lawson C, et al. 2016. Cancer Res. 76: 3826-37. PubMed
- Szargel R, et al. 2016. Hum Mol Genet. 10.1093/hmg/ddw189.. PubMed
- Damez-Werno D, et al. 2016. Proc Natl Acad Sci U S A. 113: 9623-28. PubMed
- Su P, et al. 2016. J Immunol. 197: 1054-64. PubMed
- Morozumi Y, et al. 2016. J Mol Cell Biol. 8:349-62. PubMed
- Miura Y, et al. 2016. Biochem J. 473:2591-602. PubMed
- Pashkova N, et al. 2016. Cell Rep. 17:303-15. PubMed
- Sanchez M, et al. 2016. J Biol Chem. 291:19760-73. PubMed
- Hwang J, Lee J, Pallas D 2016. J Biol Chem. 291:21008-19. PubMed
- Jiménez-Canino R, et al. 2016. J Biol Chem. 291:19068-78. PubMed
- Barquilla A, et al. 2016. Mol Biol Cell. 27:2757-70. PubMed
- Yadav S, et al. 2016. Sci Rep. 6:34100. PubMed
- Hashimoto A, et al. 2016. Oncogenesis. 0.388194444. PubMed
- Guirouilh-Barbat J, et al. 2016. PLoS Genet. 12:e1006230. PubMed
- Gómez-H L, et al. 2016. Nat Commun. 7:13298. PubMed
- Rademacher N, et al. 2016. Sci Rep. 6:35283. PubMed
- Lin Z, et al. 2016. Nat Genet. 10.1038/ng.3701. PubMed
- Kaczmarska Z, et al. 2016. Nat Chem Biol. 10.1038/nchembio.2217. PubMed
- Zee B, et al. 2016. PLoS One. 11:e0163820. PubMed
- Despras E, et al. 2016. Nat Commun. 7:13326. PubMed
- Zhang H, et al. 2016. J Neurosci. 36:11837-50. PubMed
- Zhang M, et al. 2016. Cell Res. 26:1302-19. PubMed
- Baehr C, et al. 2016. J Biol Chem. 291:26875-85. PubMed
- Boehm E, et al. 2016. J Biol Chem. 291:25877-87. PubMed
- Gail Kilroy, et al. 2016. J Biol Chem. 291:27289-97. PubMed
- Hongchen Cai, Aimin Liu 2016. Proc Natl Acad Sci U S A. 113:14751-6. PubMed
- Tam K, et al. 2016. MBio. 7:e02024-16. PubMed
- Hanke L, et al. 2016. MBio. 7(6). pii: e01569-16. PubMed
- Stoeber M, et al. 2016. Proc Natl Acad Sci U S A. 113(50):E8069-E8078. PubMed
- Gu Q, et al. 2016. J Virol. 90:10545-57. PubMed
- Ramachandran S, et al. 2016. J Biol Chem. 291: 25489-504. PubMed
- Kaufmann T, et al. 2016. J Cell Sci. 129:4607-21. PubMed
- Piwko W, et al. 2016. EMBO J. 35:2584-601. PubMed
- Matsuo Y, et al. 2016. J Cell Sci. 129:4592-606. PubMed
- Athmer J, et al. 2017. MBio. 10.1128/mBio.02320-16. PubMed
- Gao L, et al. 2017. J Cell Sci. 130:396-405. PubMed
- Gong Y, et al. 2017. Genes Dev. 31:46-58. PubMed
- K Kataoka, K Mochizuki 2017. J Cell Sci. 130:480-9. PubMed
- Liszczak G, et al. 2017. Proc Natl Acad Sci U S A. 114:681-6. PubMed
- Haggie P, et al. 2017. J Biol Chem. 292:771-85. PubMed
- T Taetzsch, et al. 2017. J Neurosci. 37:70-82. PubMed
- Yagita Y, et al. 2017. J Biol Chem. 292:691-705. PubMed
- Chong Jiang, et al. 2017. J Biol Chem. 292:3137-45. PubMed
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- Davis RB, et al. 2017. JCI Insight. 2:e92465. PubMed
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- Oishi A, et al. 2017. Sci Rep. 7:8990. PubMed
- Product Citations
-
- RRID
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AB_2801249 (BioLegend Cat. No. 901533)
AB_2565006 (BioLegend Cat. No. 901501)
AB_2565007 (BioLegend Cat. No. 901502)
AB_2565005 (BioLegend Cat. No. 901503)
Antigen Details
- Biology Area
- Cell Biology
- Gene ID
- NA
- Specificity (DOES NOT SHOW ON TDS):
- HA.11
- Specificity Alt (DOES NOT SHOW ON TDS):
- HA.11
- App Abbreviation (DOES NOT SHOW ON TDS):
- WB,FC,ICC,IP,Purification
- UniProt
- View information about HA.11 on UniProt.org
Related Pages & Pathways
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Anti-HA.11 Epitope Tag Affinity Matrix
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Alexa Fluor® 488 anti-HA.11 Epitope Tag
HA tagged CHO cells stained with Alexa Fluor® 488 Labeled An... Transfected RBL1 cells expressing the HA tag on the cell sur... -
Alexa Fluor® 594 anti-HA.11 Epitope Tag
Staining of HA.11 Clone 16B12 Monoclonal Antibody, Alexa Flu... -
Anti-HA.11 Epitope Tag
Immunofluorescence of HA.11 tagged Sbhlp protein. Photo cour... Western blot analysis of cell lysates from CHO and CHO HA st... Immunoprecipitation of HA protein from CHO and CHO-HA stable... Immunofluorescence staining of HeLa cells transfected withou... -
Biotin anti-HA.11 Epitope Tag
Total lysates (15 µg protein) from CHO (lane 1) and CHO HA s... Immunofluorescence of HA-tagged plasmid-transfected A549 cel... -
FITC anti-HA.11 Epitope Tag
Transfected RBL1 cells expressing the HA tag on the cell sur... -
Purified anti-HA.11 Epitope Tag
Western blot of Clone 16B12. Lane 1: Molecular weight marker... Staining of Clone 16B12 on methanol fixed CHO cells transfec... Immunoprecipitation of HA protein from CHO and CHO-HA stable... Transfected RBL1 cells expressing the HA tag on the cell sur... -
Alexa Fluor® 647 anti-HA.11 Epitope Tag
HA tag stably transfected CHO cells were fixed with ice cold... Transfected RBL1 cells expressing the HA tag on the cell sur... -
PE anti-HA.11 Epitope Tag
CHO-K1 cells (open histogram) or HA tag stably transfected c... Transfected RBL1 cells expressing the HA tag on the cell sur... -
Direct-Blot™ HRP anti-HA.11 Epitope Tag
Total cell lysate from CHO (lane 1) and CHO stably transfect... -
Ultra-LEAF™ Purified anti-HA.11 Epitope Tag
Total cell lysate (15 µg protein) from CHO (lane 1) and CHO ... Transfected RBL1 cells expressing the HA tag on the cell sur... -
Brilliant Violet 421™ anti-HA.11 Epitope Tag
CHO cells stably transfected with HA tag were fixed with 4% ... Transfected RBL1 cells expressing the HA tag on the cell sur... -
PE/Dazzle™ 594 anti-HA.11 Epitope Tag
CHO-K1 cells (open histogram) or HA tag stably transfected c... Transfected RBL1 cells expressing the HA tag on the cell sur... -
PE/Cyanine7 anti-HA.11 Epitope Tag
CHO-K1 cells (open histogram) or HA tag stably transfected c... Transfected RBL1 cells expressing the HA tag on the cell sur... -
Pacific Blue™ anti-HA.11 Epitope Tag
CHO-K1 cells (open histogram) or HA tag stably transfected c... Transfected RBL1 cells expressing the HA tag on the cell sur... -
APC anti-HA.11 Epitope Tag
CHO-K1 cells (open histogram) or HA tag stably transfected c... Transfected RBL1 cells expressing the HA tag on the cell sur... -
PerCP/Cyanine5.5 anti-HA.11 Epitope Tag
CHO-K1 cells (open histogram) or HA tag stably transfected c... Transfected RBL1 cells expressing the HA tag on the cell sur... -
TotalSeq™-C1131 anti-HA.11 Epitope Tag
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TotalSeq™-A1131 anti-HA.11 Epitope Tag
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TotalSeq™-B1131 anti-HA.11 Epitope Tag
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