- Clone
- MAR1-5A3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IFNAR1, IFN-αR1, IFN-α/β Receptor Subunit 1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![MAR1-5A3_LEAF_011909 MAR1-5A3_LEAF_011909](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/MAR1-5A3_LEAF_011909.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 splenocytes stained with LEAF™ purified MAR1-5A3, followed by biotinylated anti-mouse IgG and Sav-PE.
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
IFNAR-1, the type I IFN receptor subunit 1, is coexpressed with IFNAR-2 on nearly all cells and make up the heterodimeric receptor complex that binds to all type I IFNs (IFN-α/β). Type I IFNs are a group of structurally and functionally related cytokines that have been shown to promote anti-viral, anti-microbial, anti-tumor, and autoimmune responses. Ligand binding to the IFN-α/β receptor complex leads to the tyrosine phosphorylation and activation of IFNAR-1-associated Tyk2 and IFNAR-2-associated Jak1 signal transductions.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Exogenously expressed murine IFNAR1 extracellular domain
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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FC - Quality tested
IP, WB, ELISA, Block - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, ELISA1, and blocking8,9. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 127322) (Endotoxin <0.01 EU/µg).
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Application References
(PubMed link indicates BioLegend citation) -
- Sheehan KC, et al. 2006. J. Interferon Cytokine Res. 26:804. (FC, Block, IP, WB, ELISA)
- Dunn GP, et al. 2005. Nat. Immunol. 6:722. (FC, WB)
- Miller JC, et al. 2008. J. Immunol. 181:8492. PubMed
- Habjan M, et al. 2009. J. Virol. 83:4365. PubMed
- Kelly-Scumpia KM, et al. 2010. J. Exp. Med. 207:319. PubMed
- Swanson CL, et al. 2010. J. Exp. Med. 207:1485. PubMed
- Marshall HD, et al. 2011. J Virol. epub. PubMed
- Swann JB, et al. 2007. J. Immunol. 178:7540. (Block)
- Jones GW, et al. 2011. FASEB J. 25:409. (Block)
- Mitzel DN, et al. 2014. J. Immunol. 192:4273. PubMed
- Product Citations
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- RRID
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AB_2832460 (BioLegend Cat. No. 127327)
AB_2629522 (BioLegend Cat. No. 127324)
AB_2819862 (BioLegend Cat. No. 127328)
AB_11150409 (BioLegend Cat. No. 127321)
AB_11149116 (BioLegend Cat. No. 127322)
AB_2629521 (BioLegend Cat. No. 127323)
Antigen Details
- Distribution
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IFNAR1 and IFNAR2 are coexpressed on nearly all cells.
- Ligand/Receptor
- IFN-α/β
- Antigen References
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1. Branca AA, et al. 1981. Nature 294:768.
2. Orchansky P, et al. 1984. J. Interferon Res. 4:275.
3. Hemmi S, et al. 1994. Cell 76:803.
4. Novick D, et al. 1994. Cell 77:391. - Gene ID
- 15975 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- IFNAR-1
- Specificity Alt (DOES NOT SHOW ON TDS):
- IFNAR-1
- App Abbreviation (DOES NOT SHOW ON TDS):
- FC,IP,WB,ELISA,Block
- UniProt
- View information about IFNAR-1 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All IFNAR-1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse IFNAR-1 | MAR1-5A3 | FC,IP,WB,ELISA,Block |
Biotin anti-mouse IFNAR-1 | MAR1-5A3 | FC,IP,WB,ELISA |
PE anti-mouse IFNAR-1 | MAR1-5A3 | FC |
APC anti-mouse IFNAR-1 | MAR1-5A3 | FC |
Ultra-LEAF™ Purified anti-mouse IFNAR-1 | MAR1-5A3 | FC,IP,WB,ELISA,Block |
PE/Cyanine7 anti-mouse IFNAR-1 | MAR1-5A3 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse IFNAR-1
C57BL/6 splenocytes stained with purified MAR1-5A3, followed... -
Biotin anti-mouse IFNAR-1
C57BL/6 mouse splenocytes stained with biotinylated MAR1-5A3... -
PE anti-mouse IFNAR-1
C57BL/6 splenocytes stained with MAR1-5A3 PE -
APC anti-mouse IFNAR-1
C57BL/6 splenocytes stained with MAR1-5A3 APC -
Ultra-LEAF™ Purified anti-mouse IFNAR-1
C57BL/6 splenocytes stained with LEAF™ purified MAR1-5A3, fo... -
PE/Cyanine7 anti-mouse IFNAR-1
Mouse splenocyte were stained with anti-mouse IFNAR-1 (clone...
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