Alexa Fluor® 594 anti-GluR2 Antibody GluA2/GluR2 glutamate receptor , L21/32

Pricing & Availability

Clone: L21/32 (See other available formats)
Regulatory Status: RUO
Other Names: GluA2/GluR2 glutamate receptor, Glutamate receptor 2, GluR2, gluR-2, glutamate receptor B, AMPA-selective glutamate receptor 2
Isotype: Mouse IgG1, κ
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Product Citations: publications

IHC staining of Alexa Fluor® 594 anti-GluR2 antibody (clone L21/32) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 60X objective. Scale Bar: 20 µm

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810503

25 µg

104€

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810504

100 µg

169€

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Description

Glutamate receptor 2 (GluR2), also known as glutamate receptor B, is a subunit of AMPA receptors (AMPARs) involved in fast excitatory synaptic transmission. GluR2 regulates the permeability of an AMPAR to sodium, potassium, and calcium, and the absence of GluR2 allows AMPARs to be blocked by polyamines.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This monoclonal antibody was raised against a fusion protein corresponding to amino acids 834-883 of rat GluA2/GluR2 glutamate receptor.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes: It does not cross-react with GluA1/GluR1, GluA3/GluR3, or GluA4/GluR4.
Additional Product Notes: The reactivity of Alexa Fluor® 594 anti-GluR2 antibody was verified in FFPE mouse brain tissue.
RRID: AB_2801141 (BioLegend Cat. No. 810503)
AB_2801142 (BioLegend Cat. No. 810504)

Antigen Details

Structure: Expected Molecular Weight: 90 kD
Cell Type: Glutamatergic Neurons
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family: Postsynaptic proteins
Gene ID: 29627 View all products for this Gene ID
UniProt: View information about GluA2/GluR2 glutamate receptor on UniProt.org

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Related Protocols

Immunohistochemistry Protocol for Paraffin-Embedded Sections

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Other Formats

View All GluA2/GluR2 glutamate receptor Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-GluR2	L21/32	WB,IHC-P
Alexa Fluor® 594 anti-GluR2	L21/32	IHC-P

Certificate of Analysis

Go To Top Version: 1 Revision Date: 01/25/2019

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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