- Clone
- 7C6B05 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Spleen focus forming virus (SFFV) proviral integration oncogene, Transcription factor PU.1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![7C6B05_AF647_SIP1_Antibody_1_ICFC_082115 7C6B05_AF647_SIP1_Antibody_1_ICFC_082115](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/7C6B05_AF647_SIP1_Antibody_1_ICFC_082115.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were surface stained with CD19 Brilliant Violet 421™ and then were treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with anti-human SPI1 (clone 7C6B05) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom). -
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Human peripheral blood monocyte-derived macrophages were fixed with 1% paraformaldehyde (PFA) and permeabilized with 0.5% TX-100. Then the cells were stained with 5 µg/ml of SPI1 (clone 7C6B05) Alexa Fluor® 647 (red) overnight at 4°C and followed by 5 µg/ml of CD11b Alexa Fluor® 488 (green) staining for 30 minutes. Nuclei were counterstained with DAPI (blue). The image was captured with 60X objective.
SPI1 is a transcription factor belonging to the E26-transformation-specific (Ets) family and is exclusively expressed in hematopoietic cells. SPI1 regulates cell fate decisions during differentiation of hematopoietic stem cells, which is crucial for the development of lymphoid and myeloid cell lineages. SPI1-deficient mice lack macrophages, neutrophils, and B lymphocytes, and they die before or shortly after birth. Abnormally regulated expression of SPI1 can lead to developmental defects as well as occurring malignancy. Overexpression of SPI1 blocks erythroid differentiation and inhibits cell death. Mice carrying a mutant SPI1 allele show decreased SPI1 expression and develop acute myeloid leukaemia (AML), suggesting the role of SPI1 in oncogenesis.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full length human SPI1 recombinant protein
- Formulation
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µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA). - Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- µg sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining using µsize, the suggested use of this reagent is 0.5 µg per million cells in 100 µl volume. For flow cytometric staining using tests sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For immunocytochemistry, a concentration range of 2.5 - 5.0 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
- Additional Product Notes
-
View more applications data for this product in our Scientific Poster Library.
- Product Citations
-
- RRID
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AB_2563262 (BioLegend Cat. No. 658003)
AB_2563263 (BioLegend Cat. No. 658004)
AB_2563895 (BioLegend Cat. No. 658008)
Antigen Details
- Structure
- 270 amino acids, predicted molecular weight of 31 kD; contains a C-termianl ETS domain responsible for DNA binding.
- Distribution
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Nucleus.
- Function
- SPI1 is a transcription factor required for the development of lymphoid and myeloid cells.
- Interaction
- SPI1 interacts with RUNX1, CEBPD, NONO, SPIB, and GFI1.
- Cell Type
- B cells, Dendritic cells, Neutrophils
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Hikami K, et al. 2011. Arthritis Rheum. 63:755.
2. Zakrzewska A, et al. 2010. Blood 116:e1.
3. Pham TH, et al. 2013. Nucleic Acids Res. 41:6391.
4. Pospisil V, et al. 2011. EMBO J. 30:4450.
5. Zarnegar MA, et al. 2010. Mol. Cell Biol. 30:4922.
6. Rimmelé P, et al. 2010. Cancer Res. 70:6757. - Gene ID
- 6688 View all products for this Gene ID
- UniProt
- View information about SPI1 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All SPI1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Alexa Fluor® 647 anti-SPI1 (PU.1) | 7C6B05 | ICFC,ICC |
Purified anti-SPI1 (PU.1) | 7C6B05 | WB,ICC,IP |
Alexa Fluor® 594 anti-SPI1 (PU.1) | 7C6B05 | ICC,IHC-P |
PE anti-SPI1 (PU.1) | 7C6B05 | ICFC |
Alexa Fluor® 488 anti-SPI1 (PU.1) | 7C6B05 | ICFC |
PE/Cyanine7 anti-SPI1 (PU.1) | 7C6B05 | ICFC |
APC anti-SPI1 (PU.1) | 7C6B05 | ICFC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Alexa Fluor® 647 anti-SPI1 (PU.1)
Human peripheral blood lymphocytes were surface stained with... Human peripheral blood monocyte-derived macrophages were fix... -
Purified anti-SPI1 (PU.1)
Total cell lysates (15 µg protein) from THP1 (lane 1) and MC... THP-1 cells were stained with purified anti-PU.1 (clone 7C6B... Immunoprecipitation of PU.1 from THP-1 cell extracts. Lane 1... -
Alexa Fluor® 594 anti-SPI1 (PU.1)
Human peripheral blood monocyte-derived macrophages were fix... Human paraffin-embedded tonsil tissue slices were prepared w... -
PE anti-SPI1 (PU.1)
Human peripheral blood lymphocytes were surface stained with... -
Alexa Fluor® 488 anti-SPI1 (PU.1)
Human peripheral blood lymphocytes were surface stained with... -
PE/Cyanine7 anti-SPI1 (PU.1)
Human peripheral blood lymphocytes were surface stained with... -
APC anti-SPI1 (PU.1)
Human peripheral blood lymphocytes were surface stained with...
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