Loading...

PE anti-mouse Ki-67 Antibody

Pricing & Availability
Clone
16A8 (See other available formats)
Regulatory Status
RUO
Other Names
KIA, proliferation-related Ki-67 antigen
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
16A8_PE_Ki67_Antibody_ICFC_082112
C57BL/6 mouse splenocytes were stimulated for 3 days with Con-A plus IL-2; subsequently, the cells were fixed and permeabilized with 70% ethanol, and then stained with Ki-67 (clone 16A8) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
  • 16A8_PE_Ki67_Antibody_ICFC_082112
    C57BL/6 mouse splenocytes were stimulated for 3 days with Con-A plus IL-2; subsequently, the cells were fixed and permeabilized with 70% ethanol, and then stained with Ki-67 (clone 16A8) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
652403 25 µg 123€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
652404 100 µg 288€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase, while in G0 phase the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli expressed partial mouse Ki-67 recombinant protein, 1816-2163 aa.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by our Ki-67 staining protocol below. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Medina-Reyes EI, et al. 2015. Environ Res. 136:424. PubMed
  2. Guillaumond F, et al. 2015. PNAS. 112:2473. PubMed
  3. Sharma SK, et al. 2015. J Immunol. 194:5529. PubMed
  4. Rodero MP, et al. 2014. J. Invest. Dermatol. 7:1991-7. PubMed
Product Citations
  1. Wang YC, et al. 2023. J Exp Med. 220:. PubMed
  2. Lucas B, et al. 2023. Nat Commun. 14:2066. PubMed
  3. Park HJ, et al. 2023. NPJ Vaccines. 8:84. PubMed
  4. Chan S, et al. 2022. Nat Cancer. . PubMed
  5. Cautivo KM, et al. 2022. Immunity. 55:254. PubMed
  6. Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed
  7. Li Y, et al. 2022. Brain Behav Immun. 101:1. PubMed
  8. Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed
  9. Liang J, et al. 2016. Nat Med. 22:1285-1293. PubMed
  10. Sizova O, et al. 2018. Mol Cancer Res. 16:1652. PubMed
  11. Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
  12. Liu J, et al. 2015. Sci Rep. 5: 17569. PubMed
  13. Wu M, et al. 2021. Nat Commun. 12:3500. PubMed
  14. Ferretti M, et al. 2016. Brain Behav Immun. 54: 211-225. PubMed
  15. Mathur S, et al. 2021. JCI Insight. 6:. PubMed
  16. Yang F, et al. 2020. Cancer Res. 80:3677. PubMed
  17. Sharma S, et al. 2015. J Immunol. 194:5529. PubMed
  18. Sekiya T et al. 2018. Cell reports. 24(6):1627-1638 . PubMed
  19. Feng Y, et al. 2018. Kidney Dis (Basel). 4:95. PubMed
  20. Miyazaki M et al. 2017. Immunity. 46(5):818-834 . PubMed
  21. Saint Fleur–Lominy S, et al. 2018. Cell Rep. 24:3045. PubMed
  22. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  23. Schneider MK, et al. 2018. Physiol Rep. 6:e13645. PubMed
  24. Giannini S, et al. 2020. Nat Commun. 0.705555556. PubMed
  25. Bayik D, et al. 2020. Cancer Discov. 1.256944444. PubMed
  26. Soni C, et al. 2020. Immunity. 52(6):1022-1038.e7. PubMed
  27. Song J, et al. 2021. Cancer Commun (Lond). 41:867. PubMed
  28. Haas MS, et al. 2020. Mol Cancer Res. 19:717. PubMed
  29. Lee J, et al. 2020. Cell Reports. 33(13):108560. PubMed
  30. Godavarthy PS, et al. 2019. Haematologica. 104(10). PubMed
  31. Otvos B, et al. 2021. Clin Cancer Res. 27:2038. PubMed
  32. Shani O, et al. 2021. Elife. 10: . PubMed
  33. Watson MJ, et al. 2021. Nature. 591:645. PubMed
  34. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  35. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  36. King R, et al. 2021. Sci Adv. 7:eabj5293. PubMed
  37. Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
  38. Chen Z, et al. 2020. Cell Death Dis. 11:180. PubMed
  39. Sun X, et al. 2021. Nature. 599:673. PubMed
  40. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  41. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  42. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  43. Hu J, et al. 2022. J Immunother Cancer. 10:. PubMed
  44. Muzumdar S, et al. 2019. Am J Pathol. 189:568. PubMed
  45. Kim GC, et al. 2020. Front Oncol. 10:642. PubMed
  46. Fang W, et al. 2021. J Exp Clin Cancer Res. 40:4. PubMed
  47. Ghura H, et al. 2021. Neoplasia. 23:837. PubMed
  48. Zhong C, et al. 2021. J Virol. 95:e0092521. PubMed
  49. Zamarin D, et al. 2020. Clin Cancer Res. 26:4531. PubMed
  50. Lawson M, et al. 2015. Nat Commun. 6: 8983. PubMed
  51. Chen S, et al. 2015. Cancer Res . 7: 519-531. PubMed
  52. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  53. Zhang Y, et al. 2021. JCI Insight. 6:e150735. PubMed
  54. Wang X, et al. 2022. Elife. 11:. PubMed
  55. Li Z et al. 2018. Immunity. 49(4):640-653 . PubMed
  56. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  57. Nagatake T, et al. 2019. Mucosal Immunol. 12:p1082. PubMed
  58. Wang N, et al. 2020. Front Immunol. 1.765972222. PubMed
  59. Oladiran O, et al. 2021. Front Immunol. 12:720733. PubMed
  60. Karimzadeh A, et al. 2021. Front Cell Dev Biol. 9:734176. PubMed
  61. Nguyen HD, et al. 2020. Cell Rep. 33:108316. PubMed
RRID
AB_2561524 (BioLegend Cat. No. 652403)
AB_2561525 (BioLegend Cat. No. 652404)

Antigen Details

Structure
325 kD protein containing a forkhead-associated domain (FHA) and 13 tandem repeats
Distribution

Nucleus and chromosome

Function
Required for cell cycle progression and proliferation
Interaction
Interacts with KIF15; binds to MKI67IP through FHA domain
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Starborg M, et al. 1996. J. Cell. Sci. 109:143.
2. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
3. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
4. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
5. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
6. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.

Gene ID
17345 View all products for this Gene ID
UniProt
View information about Ki-67 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 4    Revision Date: 01/08/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account