- Clone
- W19086B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Erythroid 2 Like 2, NF-E2-Related Factor 2, HEBP1, IMDDHH
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W19086B_PE_NRF2_Antibody_121922 W19086B_PE_NRF2_Antibody_121922](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W19086B_PE_NRF2_Antibody_121922.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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HeLa cells were incubated overnight with (filled histogram) or without (open histogram) the proteasome inhibitor MG132. Cells were fixed with True-Nuclear™ Fix buffer and permeabilized with True-Nuclear™ Perm Buffer. Cells were then stained with anti-NRF2 (clone W19086B) PE.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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939203 | 25 tests | 113€ | ||||
939204 | 100 tests | 296€ |
NRF2 is a transcription factor that plays a critical role in inducing expression of genes required for oxidative stress defense and stress balance. It does so by binding to antioxidant response elements (AREs), which are located upstream of target genes. NRF2 is degraded by ubiquitination of KEAP1 E3 ligase. In a clinical setting, NRF2 is frequently activated in many types of cancers; it drives metabolic adaptation and survival in ROS-rich tumor microenvironments.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial recombinant human NRF2 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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ICFC – Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
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This clone was tested for ICC on 4% PFA-fixed HeLa cells and permeabilized with either methanol or Triton X-100. Both permeabilization methods were compatible with NRF2 staining.
Clone W19086B is validated to detect NRF2 in mouse NIH/3T3 cells using a Western blot on whole cell lysate. - RRID
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AB_2936521 (BioLegend Cat. No. 939203)
Antigen Details
- Structure
- NRF2 is a 605 amino acid protein with a predicted molecular weight of 67 kD.
- Distribution
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Ubiquitously expressed/Nucleus
- Function
- Transcription factor/Redox balance
- Biology Area
- Cell Biology, Mitochondrial Function, Transcription Factors
- Antigen References
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- M. da Costa R, et al. 2019. Front In Pharm. 10:3389.
- Shoemaker A. 2017. Sci Trans Med. 9:420.
- Robledinos-Antón N, et al. 2019. Hindawi. 10:1155.
- Cuadrado A, et al. 2019. Nat Rev Drug Discov. 18:295-317.
- Gene ID
- 4780 View all products for this Gene ID
- UniProt
- View information about NRF2 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All NRF2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-NRF2 | W19086B | WB,ICC,IP,IHC-P,ICFC |
PE anti-NRF2 | W19086B | ICFC |
Alexa Fluor® 647 anti-NRF2 | W19086B | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-NRF2
Whole cell lysates (15 µg) from untreated (-) or MG-132 trea... HeLa cells were incubated overnight with (filled histogram) ... Untreated (panel A) or MG-132 treated (panel B) (10 µM, 10 h... Whole cell extracts (250 µg total protein) prepared from HeL... IHC staining of purified anti-NRF2 antibody (clone W19086B) ... Whole cell lysates (15 μg) from untreated (-) or MG-132 trea... -
PE anti-NRF2
HeLa cells were incubated overnight with (filled histogram) ... -
Alexa Fluor® 647 anti-NRF2
HeLa cells untreated (negative control, open histogram), or ...
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