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PE anti-TMEM119 (Extracellular) Antibody

Pricing & Availability
Clone
A16075D (See other available formats)
Regulatory Status
RUO
Other Names
Transmembrane protein 119
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A16075D_PE_TMEM119_Antibody_1_013124
Non-transfected RBL-1 cells (negative control, open histogram), or transfected with a human TMEM119 expression vector (positive control, filled histogram) were fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and stained with PE anti-TMEM119 (clone A16075D) or PE mouse IgG2b, κ Isotype Control (open histogram, dashed line) (representative histogram for untreated or treated cells) (Cat. No. 400311).
  • A16075D_PE_TMEM119_Antibody_1_013124
    Non-transfected RBL-1 cells (negative control, open histogram), or transfected with a human TMEM119 expression vector (positive control, filled histogram) were fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and stained with PE anti-TMEM119 (clone A16075D) or PE mouse IgG2b, κ Isotype Control (open histogram, dashed line) (representative histogram for untreated or treated cells) (Cat. No. 400311).
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853315 25 tests 128€
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853316 100 tests 320€
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Description

Microglia are the resident parenchymal myeloid cells of the CNS, and play an important role in development, homeostasis, disease, and injury. TMEM119 is a transmembrane protein and was identified as a microglia-specific marker in both mouse and human CNS. Moreover, TMEM119 immunoreactivity has been exclusively detected on a subset of Iba1(+) CD68(+) microglia with ramified and amoeboid morphologies in the brains of neurodegenerative diseases. TMEM119 also plays an important role in bone formation and normal bone mineralization. It promotes the differentiation of myoblasts into osteoblasts. and it may induce the commitment and differentiation of myoblasts into osteoblasts. TMEM119 is also essential for normal spermatogenesis and late testicular differentiation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human TMEM119 protein (extracellular domain)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our Cyto-Fast™ Fix/Perm Buffer Set. For flow cytometric staining, the suggested use of this reagent is 5 µL per 106 cells in 100 µL volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

For microscopy, the antibody was found to work best when using fresh frozen human tissues. Communication by Dr. Frederick Christian Bennett at Stanford University, under review in Neuron.

*The human TMEM119 full length plasmid was a courtesy gift from Dr. Ben Barres at Stanford University.

Application References

(PubMed link indicates BioLegend citation)

  1. Bennett FC, et al. 2018. Neuron. 98:1-14 PubMed

Antigen Details

Structure
TMEM119 is an 283 amino acid protein with a molecular mass of 30 kD.
Distribution

Tissue distribution: TMEM119 is expressed in the brain, bone, and lymphoid tissues.
Cellular distribution: Membrane and endoplasmic reticulum.

Function
TMEM119 is an important microglia marker and is implicated in neurodegenerative diseases.
Interaction
TMEM119 interacts with SMAD1, SMAD5 and RUNX2.
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Antigen References
  1. Bennett ML, et al. 2016. Proc Natl Acad Sci U S A. 113
  2. Satoh J, et al. 2016. Neuropathology. 36:39-49
  3. Kawao N and Kaji H. 2015. J Cell Biochem. 116:687
  4. Zrzavy T, et al. 2017. Brain. 140:1900-1913
Gene ID
338773 View all products for this Gene ID
UniProt
View information about TMEM119 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 01/31/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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