- Clone
- 2G10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- RNA pol sigma 70, rpoD, sigma D, RNA Polymerase
- Isotype
- Mouse IgG2b
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Whole cell extracts (15 µg protein) from BL21 E. coli cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-E. coli RNA Sigma 70 antibody, clone 2G10, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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663207 | 25 µg | 90€ | ||||
663208 | 100 µg | 259€ |
RNA polymerase transcribes DNA to synthesize RNA. Prokaryotes contain a single RNA polymerase- core RNA polymerase. RNA polymerase sigma 70 (also known as RNA pol sigma 70 and rpoD) is a part of a family of sigma factor subunits that are required for specific promoter recognition and initiation. RNA pol sigma 70 is the major housekeeping sigma factor responsible for RNA polymerase recognition out of the majority of promoters necessary to transcribe bacterial genes which are expressed during expansion.
Product DetailsProduct Details
- Verified Reactivity
- E. coli
- Reported Reactivity
- Other species
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Clone 2G10 is extremely useful for identifying the major sigma factor from a variety of bacteria. It does not react with Borellia burgdorferi. Clone 2G10 recognizes epitope 470-486.
Additional reported applications (for the relevant formats) include: functional assay and immunoprecipitation. -
Application References
(PubMed link indicates BioLegend citation) -
- Breyer MJ, et al. 1997. J. Bacterial 179:1404.
- Lesley SA, et al. 1989. J. Biol. Chem. 264:3794.
- Strickland MS, et al. 1988. Biochemistry 27:5755.
- Product Citations
-
- RRID
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AB_2814498 (BioLegend Cat. No. 663207)
AB_2814499 (BioLegend Cat. No. 663208)
Antigen Details
- Structure
- Sigma 70 (σ70) is a 70 kD transcription factor of E. coli RNA polymerase.
- Biology Area
- Cell Biology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Gene ID
- 947567 View all products for this Gene ID
- UniProt
- View information about Sigma 70 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Sigma 70 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Direct-Blot™ HRP anti-E. coli RNA Sigma 70 | 2G10 | WB |
Purified anti-E. coli RNA Sigma 70 | 2G10 | WB |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Direct-Blot™ HRP anti-E. coli RNA Sigma 70
15 µg of total protein extract from E. coli was res... -
Purified anti-E. coli RNA Sigma 70
Whole cell extracts (15 µg protein) from BL21 E. coli...
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