Purified anti-HSF2 Antibody (Previously Covance catalog# MRT-5114)

Pricing & Availability
Clone
3E2 (See other available formats)
Regulatory Status
RUO
Other Names
Heat shock factor protein 2, HSF 2, HSTF 2, heat shock transcription factor 2
Previously
Covance Catalog# MRT-5114
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
3E2_Purified_HSF2_Antibody_WB_022315
Western blot of purified anti-HSF2 antibody (clone 3E2). Lane 1: Molecular weight marker; Lane 2: 30 µg of HeLa lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 3E2_Purified_HSF2_Antibody_WB_022315
    Western blot of purified anti-HSF2 antibody (clone 3E2). Lane 1: Molecular weight marker; Lane 2: 30 µg of HeLa lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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829301 100 µL 220€
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Description

Heat shock factor protein 2 (HSF2) is a transcription factor that enters the nucleus and binds to heat shock factor promoter elements, consisting of a consensus tandem array sequence of three oppositely oriented "AGAAN" motifs, during heat shock in eukaryotes.  Under normal conditions, it exists as a homodimer in the cytoplasm of cells where it binds to heat shock proteins and inactivates them.  During high temperature cellular stress, misfolding of proteins occurs, which causes heat shock proteins to dissociate from the heat shock factor proteins and bind to the misfolded proteins.  This leads to free heat shock proteins, which can then enter the nucleus and activate transcription. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
This monoclonal antibody was raised against mouse recombinant HSF-2 protein.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB).

Application References

(PubMed link indicates BioLegend citation)
  1. Sistonen L, et al. 1992. Mol Cell Biol. 12(9): 4104-11.
RRID
AB_2564928 (BioLegend Cat. No. 829301)

Antigen Details

Structure
Expected MW: 70 kD
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family
Heat Shock Proteins
Gene ID
3298 View all products for this Gene ID
UniProt
View information about HSF2 on UniProt.org
Go To Top Version: 5    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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