- Clone
- 6H6 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IL-3Rα, IL-3 Receptor alpha
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![6H6_Purified_CD123_CyTOF_Antibody_112113 6H6_Purified_CD123_CyTOF_Antibody_112113](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/6H6_Purified_CD123_CyTOF_Antibody_112113.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human lysed whole blood stained with 151Eu anti-CD123 (6H6) and 172Yb anti-CD38 (HIT2). Total cells, excluding lymphocytes, are displayed in the analysis.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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306027 | 100 µg | 104€ |
CD123 is the 70 kD transmembrane α chain of the IL-3 receptor. Alone, CD123 binds IL-3 with low affinity; when CD123 associates with CD131 (common β chain), it binds IL-3 with high affinity. CD123 does not transduce intracellular signals upon binding IL-3 and requires the β chain for this function. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, megakaryocytes, and some B cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human IL-3Rα transfected COS cells.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
CyTOF® - Verified - Recommended Usage
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This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
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Clone 6H6 does not inhibit IL-3 binding to low- or high-affinity IL-3Rs. Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, and immunohistochemical staining of acetone-fixed frozen sections2 and also paraformaldehyde fixed paraffin embedded tissue7.
- Additional Product Notes
-
Maxpar® is a registered trademark of Standard BioTools Inc.
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Application References
(PubMed link indicates BioLegend citation) -
- Sun Q, et al. 1996. Blood 87:83. (IP, WB)
- Herling M, et al. 2003. Blood 101:5007. (IHC)
- Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
- Martin-Gayo E, et al. 2010. Blood 115:5366. PubMed
- Chen SC, et al. 2010. Arch Dermatol Res. 302:113. PubMed
- Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed
- Peduzzi E, et al. 2007. J. Invest. Dermatol. 127:638. (IHC)
- Product Citations
-
- RRID
-
AB_2562823 (BioLegend Cat. No. 306027)
Antigen Details
- Structure
- Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70 kD
- Distribution
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Myeloid precursors, basophils, mast cells, macrophages, dendritic cells, megakaryocytes, subset of lymphocytes
- Function
- Hematopoietic cell proliferation, differentiation
- Ligand/Receptor
- IL-3
- Cell Type
- Basophils, Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, Macrophages, Mast cells, Megakaryocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Miyajima A, et al. 1993. Blood 82:1960.
- Gene ID
- 3563 View all products for this Gene ID
- UniProt
- View information about CD123 on UniProt.org
Related FAQs
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
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We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
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We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
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We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
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The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-human CD123
Human peripheral blood lymphocytes stained with 6H6 PE and C... -
PE anti-human CD123
Human peripheral blood lymphocytes stained with 6H6 PE and C... -
Purified anti-human CD123
Human peripheral blood lymphocytes stained with 6H6 PE and C... -
PE/Cyanine5 anti-human CD123
Human peripheral blood lymphocytes stained with CD45RO FITC ... -
PE/Cyanine7 anti-human CD123
Human peripheral blood lymphocytes stained with CD45RO FITC ... -
APC anti-human CD123
CD14- human peripheral blood mononuclear cells stained with ... -
FITC anti-human CD123
Human peripheral blood leukocytes stained with CD45RO APC an... -
PerCP/Cyanine5.5 anti-human CD123
Human peripheral blood lymphocytes stained with 6H6 PerCP/Cy... -
Brilliant Violet 421™ anti-human CD123
Human peripheral blood leukocytes were stained with HLA-DR F... -
Brilliant Violet 650™ anti-human CD123
Human peripheral blood leukocytes were stained with HLA-DR F... -
Brilliant Violet 510™ anti-human CD123
Human peripheral blood lymphocytes were stained with CD45RO ... -
Alexa Fluor® 647 anti-human CD123
Human peripheral blood lymphocytes were stained with CD45RO ... -
Brilliant Violet 605™ anti-human CD123
Human peripheral blood lymphocytes were stained with HLA-DR ... -
Purified anti-human CD123 (Maxpar® Ready)
Human lysed whole blood stained with 151Eu anti-CD123 (6H6) ... -
Brilliant Violet 711™ anti-human CD123
Human peripheral blood lymphocytes were stained with CD45RO ... -
Brilliant Violet 785™ anti-human CD123
Human peripheral blood lymphocytes were stained with CD45RO ... -
PE/Dazzle™ 594 anti-human CD123
Human peripheral blood lymphocytes were stained with CD45RO ... -
Alexa Fluor® 488 anti-human CD123
Human peripheral blood lymphocytes were stained with CD45RO ... -
PE/Cyanine7 anti-human CD123
Typical results from human peripheral blood lymphocytes stai... -
TotalSeq™-A0064 anti-human CD123
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Alexa Fluor® 700 anti-human CD123
Human lysed whole blood was stained with CD45RO FITC and CD... -
APC/Fire™ 750 anti-human CD123
Human lysed whole blood was stained with CD45RO FITC and CD1... -
Pacific Blue™ anti-human CD123
Human lysed whole blood was stained with CD45RO FITC and CD1... -
TotalSeq™-C0064 anti-human CD123
-
TotalSeq™-B0064 anti-human CD123
-
TotalSeq™-D0064 anti-human CD123
-
PerCP anti-human CD123
Human peripheral blood lymphocytes were stained with CD45RO ... -
GMP PE/Cyanine7 anti-human CD123
Typical results from human peripheral blood lymphocytes stai... -
APC/Fire™ 810 anti-human CD123 Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
APC anti-human CD123
Typical results from Human peripheral blood lymphocytes stai... -
PE anti-human CD123
Typical results from Human peripheral blood lymphocytes stai... -
Pacific Blue™ anti-human CD123
Typical results from Human peripheral blood lymphocytes stai... -
Spark Red™ 718 anti-human CD123 (Flexi-Fluor™)
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