- Clone
- TRA-1-81 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Podocalyxin
- Isotype
- Mouse IgM, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![a-Tra-1-81_Pure_061208.jpg a-Tra-1-81_Pure_061208.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/Tra-1-81_Pure_061208.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human teratocarcinoma cell line, NCCIT, stained with purified TRA-1-81, followed by anti-mouse IgM PE -
Western blot analysis of NTERA-2 (lane 1), NF-1 (lane 2) and Hela (lane 3) cells using anti-TRA-1-81 antibody (TRA-1-81). GAPDH (poly6314) was used a loading control. -
HUES-9 ES cell colony was stained with Tra-1-81 antibody at 1:400 and then secondarily stained with Alexa Fluor® 546 goat anti-mouse IgM at 1:250 (red). Nuclei were counterstained with DAPI (blue). Image was provided by Eric O'Connor and Karl Marquez, Sanford Consortium for Regenerative Medicine.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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330702 | 100 µg | 220€ |
The TRA-1-81 antibody reacts with a 200-240 kD cell surface antigen expressed by human embryonic stem cells (ESC), embryonic germ cells (EG), and embryonal carcinoma cells. The expression of TRA-1-81 on human ESC is downregulated upon differentiation. Further studies have shown that TRA-1-81 is a neuroaminidase-resistant carbohydrate epitope expressed on podocalyxin, a member of mucin-like glycoprotein with CD34 family. Podocalyxin is a type I tranmembrane sialoprotein with anti-adhesion property.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human embryonic carcinoma cell line 2102Ep cl.2A6
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
WB - Verified
IP, ICC, IHC, ELISA - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported applications (for the relevant formats) include: immunofluorescence cell staining1, immunoprecipitation1, Western blotting1,2, ELISA1, and immunohistochemistry1 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded sections.
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Application References
(PubMed link indicates BioLegend citation) -
- Andrews PW, et al. 1984. Hybridoma. 3:347.
- Schopperle WM and Dewolf WC. 2007. Stem Cells. 25:723.
- Product Citations
-
- RRID
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AB_1089240 (BioLegend Cat. No. 330702)
Antigen Details
- Structure
- Mucin-like protein, CD34 family member, 200-240 kD
- Distribution
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embryonic stem cells (ESC), embryonic germ cells (EG), embryonal carcinoma cells
- Function
- Anti-adhesion
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Cell Biology, Immunology, Stem Cells
- Antigen References
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1. Andrews PW, et al. 1984. Hybridoma. 3:347.
2. Schopperle WM and Dewolf WC. 2007. Stem Cells. 25:723.
3. Takeda T, et al. 2000. Mol. Biol. Cell. 11:3219. - Gene ID
- 5420 View all products for this Gene ID
- UniProt
- View information about TRA-1-81 on UniProt.org
Related FAQs
Other Formats
View All TRA-1-81 Reagents Request Custom ConjugationDescription | Clone | Applications |
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Purified anti-human TRA-1-81 | TRA-1-81 | FC,WB,IP,ICC,IHC,ELISA |
Alexa Fluor® 647 anti-human TRA-1-81 | TRA-1-81 | FC,ICC |
PE anti-human TRA-1-81 | TRA-1-81 | FC |
Alexa Fluor® 488 anti-human TRA-1-81 | TRA-1-81 | FC |
TotalSeq™-A1039 anti-human TRA-1-81 | TRA-1-81 | PG |
TotalSeq™-C1039 anti-human TRA-1-81 | TRA-1-81 | PG |
TotalSeq™-B1039 anti-human TRA-1-81 | TRA-1-81 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human TRA-1-81
Human teratocarcinoma cell line, NCCIT, stained with purifie... Western blot analysis of NTERA-2 (lane 1), NF-1 (lane 2) and... HUES-9 ES cell colony was stained with Tra-1-81 antibody at ... -
Alexa Fluor® 647 anti-human TRA-1-81
NCCIT (human teratocarcinoma cell line) stained with TRA-1-8... -
PE anti-human TRA-1-81
NCCIT (human teratocarcinoma cell line) stained with TRA-1-8... -
Alexa Fluor® 488 anti-human TRA-1-81
NCCIT (human teratocarcinoma) cell line was stained with TRA... -
TotalSeq™-A1039 anti-human TRA-1-81
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TotalSeq™-C1039 anti-human TRA-1-81
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TotalSeq™-B1039 anti-human TRA-1-81
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