- Clone
- RP-2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- GMP-140, platelet activation-dependent granule membrane protein (PADGEM), GMP140
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![RP-2_Purified_CD62P_Antibody_FC_092915 RP-2_Purified_CD62P_Antibody_FC_092915](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/RP-2_Purified_CD62P_Antibody_FC_092915.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Thrombin stimulated rat platelets were stained with purified CD62P (clone RP-2) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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206002 | 100 µg | 141€ |
CD62P is a 140 kD type I transmembrane glycoprotein that is also known as P-selectin, platelet activation-dependent granule membrane protein (PADGEM), and GMP-140. It is expressed on activated platelets, megakaryocytes, and endothelial cells. CD62P is primarily stored in secretory α-granules in platelets and Weibel-Palade bodies in endothelial cells, and is rapidly relocated to the plasma membrane upon activation. The ligands for CD62P are CD162 and CD24. CD62P's primary functions are cell adhesion during neutrophil rolling, and platelet-neutrophil and platelet-monocyte interactions.
Product DetailsProduct Details
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Thrombin activated rat platelets
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
ICC, IHC-F, ELISA - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported applications (for the relevant formats of this clone) include: immunofluorescence staining2, immunohistochemistry of acetone-fixed frozen sections2, and ELISA capture3.
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Application References
(PubMed link indicates BioLegend citation) -
- Walter UM, et al. 1997. Immunology. 92:290. (Blocking function)
- Morise Z, et al. 1998. Am. J. Physiol. 274:G246. (IF, IHC - frozen)
- Walter UM, et al. 1997. Hybridoma. 16:249. (ELISA capture)
- Product Citations
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- RRID
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AB_2564112 (BioLegend Cat. No. 206002)
Antigen Details
- Structure
- Type I transmembrane glycoprotein, 140 kD
- Distribution
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Activated platelets, megakaryocytes, and endothelial cells.
- Function
- Adhesion, neutrophil rolling, platelet-neutrophil, and platelet-monocyte interactions.
- Ligand/Receptor
- CD162 (PSGL-1), CD24, and sialylated Lewis X
- Cell Type
- Endothelial cells, Megakaryocytes, Platelets
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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1. McEver R, et al. 1995. J. Biol. Chem. 270:11025.
2. Varki A. 1994. Proc. Natl. Acad. Sci. USA 91:7390. - Gene ID
- 25651 View all products for this Gene ID
- UniProt
- View information about CD62P on UniProt.org
Other Formats
View All CD62P Reagents Request Custom ConjugationDescription | Clone | Applications |
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Purified anti-rat CD62P (P-selectin) | RP-2 | FC,ICC,IHC-F,ELISA |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-rat CD62P (P-selectin)
Thrombin stimulated rat platelets were stained with purified...
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