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Alexa Fluor® 555 Donkey anti-rabbit IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4064 (See other available formats)
Regulatory Status
RUO
Isotype
Donkey Polyclonal Ig
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Product Citations
publications
Poly4064_AF555_IgG_Antibody_IF_030515
HeLa cells were fixed with 1% paraformaldehyde for ten minutes followed by permeabilization for ten minutes with 0.5% Triton X-100, and then blocked with 5% FBS for 30 minutes. 2.5 µg/ml of purified BRCA-1 (Poly26599) was added to the cells and left at 4°C overnight. The cells were then stained with 2.5 µg/ml of Alexa Fluor® 555 (Yellow) for 2 hours at room temperature. Nuclei were counterstained with DAPI and are shown in blue and phalloidin shown in red. The image was captured with a 40X objective.
  • Poly4064_AF555_IgG_Antibody_IF_030515
    HeLa cells were fixed with 1% paraformaldehyde for ten minutes followed by permeabilization for ten minutes with 0.5% Triton X-100, and then blocked with 5% FBS for 30 minutes. 2.5 µg/ml of purified BRCA-1 (Poly26599) was added to the cells and left at 4°C overnight. The cells were then stained with 2.5 µg/ml of Alexa Fluor® 555 (Yellow) for 2 hours at room temperature. Nuclei were counterstained with DAPI and are shown in blue and phalloidin shown in red. The image was captured with a 40X objective.
See Alexa Fluor® 555 spectral data
Cat # Size Price Quantity Check Availability Save
406412 100 µg 113€
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Description

This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Rabbit
Antibody Type
Polyclonal
Host Species
Donkey
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 555 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 555 has an excitation maximum of 555 nm, and a maximum emission of 565 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.

Product Citations
  1. Biering SB et al. 2017. Cell host & microbe. 22(1):74-85 . PubMed
  2. Brown CW, et al. 2020. Developmental Cell. 51(5):575-586.e4.. PubMed
  3. Baba T, et al. 2020. eLife. 9:e54257.. PubMed
  4. Divito SJ, et al. 2020. J Clin Invest. 130:4624. PubMed
  5. Neufert C, et al. 2020. Nature Protocols. 16(1):61-85. PubMed
  6. Kissane RWP, et al. 2021. Journal of Anatomy. . PubMed
  7. Son JS, et al. 2020. Cell Rep. 33:108461. PubMed
  8. Tayoun T, et al. 2022. JCI Insight. 7: . PubMed
  9. Frigon ÉM, et al. 2022. Front Neuroanat. 16:957358. PubMed
  10. Chae SA, et al. 2023. J Physiol. 601:2371. PubMed
  11. Koop K, et al. 2023. Front Immunol. 14:1163198. PubMed
RRID
AB_2563181 (BioLegend Cat. No. 406412)

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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