- Clone
- TRA-1-60-R (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Podocalyxin, TRA-1
- Isotype
- Mouse IgM, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![Tra-1-60-R_A647_102108.jpg Tra-1-60-R_A647_102108.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/Tra-1-60-R_A647_102108.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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NCCIT (human teratocarcinoma cell line) stained with TRA-1-60-R Alexa Fluor® 647 (red line) or mouse IgM, κ Alexa Fluor® 647 isotype control (blue line).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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330605 | 25 tests | 132€ | ||||
330606 | 100 tests | 278€ |
TRA-1-60-R antibody recognizes the neuraminidase resistant form of TRA-1-60. TRA-1-60 is a 200-240 kD cell surface antigen expressed by human embryonic stem cells (ESC), embryonic germ cells (EG), and embryonal carcinoma cells. The expression of TRA-1-60 on human ESC is downregulated upon differentiation. The soluble form of TRA-1-60 has been found in the serum of patients with embryonic cancers. Historically, TRA-1-60 is a neuroaminidase-sensitive (TRA-1-60-S) carbohydrate epitope expressed on podocalyxin, a member of the mucin-like glycoprotein CD34 family. Podocalyxin is a type I transmembrane sialoprotein with anti-adhesion properties. Further studies have shown that some of TRA-1-60 is neuraminidase resistant (TRA-1-60-R). The two forms of TRA-1-60 are essentially indistinguishable in terms of their cellular reactivity except for the difference in neuraminidase sensitivity. Both of TRA-1-60-S and TRA-1-60-R appear to be the same molecular weight glycoproteins detected by Western blot.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human embryonal carinoma cell line 2102Ep cl.2A6
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Red Laser (633 nm)
- Application Notes
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Additional reported applications (for the relevant formats) include: immunofluorescence microscopy, immunohistochemical staining, acetone-fixed frozen sections, immunoprecipitation, and radioimmunoassay.
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Application References
(PubMed link indicates BioLegend citation) -
- Miyoshi N, et. al. 2010. P. Natl. Acad. Sci. USA 107:40. (IF)
- Kuai XL, et al. 2009. Differentiation 77:229. (WB)
- King F, et al. 2009. Stem Cells Dev. 18:1441. (FC)
- Product Citations
- RRID
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AB_1227813 (BioLegend Cat. No. 330605)
AB_1227812 (BioLegend Cat. No. 330606)
Antigen Details
- Distribution
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Embryonic stemcells
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Immunology, Stem Cells
- Gene ID
- 5420 View all products for this Gene ID
- UniProt
- View information about TRA-1-60-R on UniProt.org
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